DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Nature 2007 imex curation Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. DIP original experiment: DIP-253795X Nature 2007 IMEx RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Nature 2007 imex curation Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. DIP original experiment: DIP-253793X Nature 2007 IMEx RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Nature 2007 imex curation Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. DIP original experiment: DIP-253794X Nature 2007 IMEx RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Nature 2007 imex curation Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. DIP original experiment: DIP-253791X Nature 2007 IMEx RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. Nature 2007 imex curation Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. Only protein-protein interactions in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Matthews AG., Kuo AJ., Ramón-Maiques S., Han S., Champagne KS., Ivanov D., Gallardo M., Carney D., Cheung P., Ciccone DN., Walter KL., Utz PJ., Shi Y., Kutateladze TG., Yang W., Gozani O., Oettinger MA. DIP original experiment: DIP-253792X Nature 2007 IMEx RAG2 PHD finger couples histone H3 lysine 4 trimethylation with V(D)J recombination. h3k9-2 Histone H3 N-terminal Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLA rag2_mouse V(D)J recombination-activating protein 2 Rag2 Rag-2 protein protein mouse Mus musculus Mouse MSLQMVTVGHNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHFDIKQNHLKLKPAIFSKDSCYLPPLRYPATCSYKGSIDSDKHQYIIHGGKTPNNELSDKIYIMSVACKNNKKVTFRCTEKDLVGDVPEPRYGHSIDVVYSRGKSMGVLFGGRSYMPSTQRTTEKWNSVADCLPHVFLIDFEFGCATSYILPELQDGLSFHVSIARNDTVYILGGHSLASNIRPANLYRIRVDLPLGTPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMVCSLVSLGDNTIEISEMETPDWTSDIKHSKIWFGSNMGNGTIFLGIPGDNKQAMSEAFYFYTLRCSEEDLSEDQKIVSNSQTSTEDPGDSTPFEDSEEFCFSAEATSFDGDDEFDTYNEDDEDDESVTGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLEERTLIHLSEGSNKYYCNEHVQIARALQTPKRNPPLQKPPMKSLHKKGSGKVLTPAKKSFLRRLFD DIP protein P21784 original sequence version: 66 DIP protein P21784 original sequence version: 84 DIP protein P21784 original sequence version: 97 DIP protein P21784 original sequence version: 126 DIP protein P21784 original sequence version: 111 mint homomint 51086F95A4A664A7 h3k4me3-rag2-1 4 7 unspecified role unspecified role unspecified role unspecified role Region 414-487 Region 414-487 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction h3k4me3-rag2-2 2 7 unspecified role unspecified role unspecified role unspecified role Region 414-527 Region 414-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 1-12 Region 1-12 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm K4me2 K4me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm K4me1 K4me1 me1lys monomethylated L-lysine Me1Lys Methyl Methylation methylk certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm in vitro In vitro direct interaction direct interaction h3k4me3-rag2-3 5 7 unspecified role unspecified role prey prey Region 414-527 Region 414-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro direct interaction direct interaction h3k4me3-rag2-4 1 7 unspecified role unspecified role prey prey Region 414-527 Region 414-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro direct interaction direct interaction h3k4me3-rag2-5 3 7 unspecified role unspecified role prey prey Region 414-487 Region 414-487 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P21784:p.Tyr415Ala Y415A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y415A'. Wed May 02 16:23:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr415ala' Tue Jun 30 17:03:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr415Ala' P21784:p.Met443Ala M443A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M443A'. Wed May 02 17:33:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met443ala' Tue Jun 30 19:04:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met443Ala' P21784:p.Trp453Ala W453A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W453A'. Wed May 02 18:44:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp453ala' Tue Jun 30 18:44:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp453Ala' P21784:p.Trp453Arg W453R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W R This feature has been corrected as a result of our quality control procedures. The original label was 'W453R'. Wed May 02 19:22:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp453arg' Tue Jun 30 17:33:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp453Arg' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro direct interaction direct interaction