DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu How kinesin waits between steps. How kinesin waits between steps. Nature 2007 imex curation Mori T., Vale RD., Tomishige M. Only protein-protein interactions ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253782X Nature 2007 IMEx How kinesin waits between steps. How kinesin waits between steps. How kinesin waits between steps. Nature 2007 imex curation Mori T., Vale RD., Tomishige M. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253784X Nature 2007 IMEx How kinesin waits between steps. How kinesin waits between steps. How kinesin waits between steps. Nature 2007 imex curation Mori T., Vale RD., Tomishige M. Only protein-protein interactions ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253783X Nature 2007 IMEx How kinesin waits between steps. How kinesin waits between steps. How kinesin waits between steps. Nature 2007 imex curation Mori T., Vale RD., Tomishige M. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Mori T., Vale RD., Tomishige M. DIP original experiment: DIP-253785X Nature 2007 IMEx How kinesin waits between steps. kinh_human Kinesin-1 heavy chain KIF5B KNS KNS1 Ubiquitous kinesin heavy chain Conventional kinesin heavy chain protein protein human Homo sapiens Human MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV DIP protein P33176 original sequence version: 148 DIP protein P33176 original sequence version: 65 DIP protein P33176 original sequence version: 133 DIP protein P33176 original sequence version: 102 DIP protein P33176 original sequence version: 152 A1FE5760C3250C8B kif5b-1 1 5 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Cy3-label Cy3-label cy3 label cy3 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Cy5-label Cy5-label cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kif5b-kif5b-2 2 5 unspecified role unspecified role prey prey Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 5 unspecified role unspecified role bait bait Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association kif5b-2 3 5 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Cy3-label Cy3-label cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Cy5-label Cy5-label cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction kif5b-kif5b-3 4 5 unspecified role unspecified role bait bait Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey Region 1-490 Region 1-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association