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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Conformational dynamics of the KcsA potassium channel governs gating properties.</fullName>
        </names>
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          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="17922011" refType="primary-reference" refTypeAc="MI:0358"/>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Conformational dynamics of the KcsA potassium channel governs gating properties.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature structural &amp; molecular biology</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Baker KA.,  Tzitzilonis C.,  Kwiatkowski W.,  Choe S.,  Riek R.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
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        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-8997" refType="imex-primary" refTypeAc="MI:0662"/>
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        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>comigration in sds</shortLabel>
            <fullName>comigration in sds page</fullName>
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          <xref>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Baker KA., Tzitzilonis C., Kwiatkowski W., Choe S., Riek R.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-68102X</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature structural &amp; molecular biology</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Conformational dynamics of the KcsA potassium channel governs gating properties.</attribute>
        </attributeList>
      </experimentDescription>
      <experimentDescription id="2">
        <names>
          <fullName>Conformational dynamics of the KcsA potassium channel governs gating properties.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="17922011" refType="primary-reference" refTypeAc="MI:0358"/>
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            <attribute name="publication title" nameAc="MI:1091">Conformational dynamics of the KcsA potassium channel governs gating properties.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature structural &amp; molecular biology</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Baker KA.,  Tzitzilonis C.,  Kwiatkowski W.,  Choe S.,  Riek R.</attribute>
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            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-8997" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-68103X" refType="identity" refTypeAc="MI:0356"/>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>solution sedimentati</shortLabel>
            <fullName>cosedimentation in solution</fullName>
            <alias type="synonym" typeAc="MI:1041">solution sedimentati</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0028" refType="identity" refTypeAc="MI:0356"/>
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            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Baker KA., Tzitzilonis C., Kwiatkowski W., Choe S., Riek R.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-68103X</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature structural &amp; molecular biology</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Conformational dynamics of the KcsA potassium channel governs gating properties.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="3">
        <names>
          <shortLabel>kcsa_strli</shortLabel>
          <fullName>pH-gated potassium channel KcsA</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Streptomyces lividans K+ channel</alias>
          <alias type="gene name" typeAc="MI:0301">kcsA</alias>
          <alias type="gene name synonym" typeAc="MI:0302">skc1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P0A334" version="SP_34" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="MINT-7259978" refType="intact-secondary"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q54397" version="SP_34" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="mint" dbAc="MI:0471" id="P0A334"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005249" version="SP_85"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008076" version="SP_85"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_85"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR013099" version="SP_85"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5EC2" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5J9P" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5VK6" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5VKE" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5VKH" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6NFU" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6NFV" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6PA0" version="SP_85"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6W0B" version="SP_85"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6W0D" version="SP_85"/>
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          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6W0A" version="SP_85"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-29626N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="1916">
          <names>
            <shortLabel>strli</shortLabel>
            <fullName>Streptomyces lividans</fullName>
          </names>
        </organism>
        <sequence>MPPMLSGLLARLVKLLLGRHGSALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGREQERRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 27</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 30</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 31</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 40</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 36</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 39</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 48</attribute>
          <attribute name="remark-internal">DIP protein P0A334 original sequence version: 66</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="crc64">DEBD9E64384BF40C</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-8997-2">
        <names>
          <shortLabel>kcsa-2</shortLabel>
        </names>
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          <primaryRef db="dip" dbAc="MI:0465" id="DIP-59108E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15663301" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-8997-2" refType="imex-primary" refTypeAc="MI:0662"/>
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        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
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                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
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            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
      <interaction id="6" imexId="IM-8997-1">
        <names>
          <shortLabel>kcsa-3</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-59108E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15663317" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-8997-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>2</experimentRef>
        </experimentList>
        <participantList>
          <participant id="7">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>