DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature structural & molecular biology 2007 imex curation Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. DIP original experiment: DIP-68097X Nature structural & molecular biology 2007 IMEx A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature structural & molecular biology 2007 imex curation Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. DIP original experiment: DIP-68100X Nature structural & molecular biology 2007 IMEx A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature structural & molecular biology 2007 imex curation Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. DIP original experiment: DIP-68101X Nature structural & molecular biology 2007 IMEx A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature structural & molecular biology 2007 imex curation Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. DIP original experiment: DIP-68098X Nature structural & molecular biology 2007 IMEx A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature structural & molecular biology 2007 imex curation Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. DIP original experiment: DIP-68096X Nature structural & molecular biology 2007 IMEx A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. Nature structural & molecular biology 2007 imex curation Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Till S., Lejeune E., Thermann R., Bortfeld M., Hothorn M., Enderle D., Heinrich C., Hentze MW., Ladurner AG. DIP original experiment: DIP-68099X Nature structural & molecular biology 2007 IMEx A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain. ago1_human Protein argonaute-1 AGO1 Argonaute RISC catalytic component 1 EIF2C1 Eukaryotic translation initiation factor 2C 1 Putative RNA-binding protein Q99 protein protein human Homo sapiens Human MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA DIP protein Q9UL18 original sequence version: 46 DIP protein Q9UL18 original sequence version: 57 DIP protein Q9UL18 original sequence version: 80 DIP protein Q9UL18 original sequence version: 99 1DBB524AE7CBAF66 tas3_schpo RNA-induced transcriptional silencing complex protein tas3 SPBC83.03c tas3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEKGIKKYLPSLPFLACISDFPENHGTSRRSATVSLERVHELFTEHWLSNLKNRREKRQELAEEAVYCRSEMLSQRKLLAAVDFPQQLKNSPAKAKATHTSSGVTKEVRASKKYTSSNVEFPLVTDGKEKPVKSKQLRKNSVTEFEKPIETKKSKHRKSRNKFLDKSSGSMEIESWDNSTSDSIIESSSRLHESISLRENDIRSSDSKSVGWDDNSTGFRESSKSLDHTDTSMFMELDSNSDPQFRPKYQAKSSWFAPDDPEASWGNLDDGWGETNGSWSSTDDTKHYKNEWAESINLDNFNRPSQQEDYDKPKNTQVSRSSNHHRRYDSYHPDSRSDSYRSKREHYDNRDTGPRSKHLEKSSYVYNQNFEDRTHLSDHGAHFHLGNANDFNMQGSSRKRKASDRQRESRENELPTKKLNASDSHNPLASLTTDKNDLYINWLKSLSFFQTNSSCAEALVKVIPHYHNKLIDFSQVLQLVFSASEKFPIQENQPLPEQLMFLSNLEKQTPFAKAVGSSIYKLVTGKNLSLDFASQILKEASILEHKNEK 4569CF59851A22A0 4569CF59851A22A0 q9upq9-2 Trinucleotide repeat-containing gene 6B protein TNRC6B KIAA1093 protein protein human Homo sapiens Human MQTNEGEVSEESSSKVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPCTAPGANPNNAQVTGALLQSESGTAPVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNTPANAPNAMKPNSKSMQDGWGESDGPVTGARHPSWEEEEDGGVWNTTGSQGSASSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVGSLSDKKFDVDKRAMNLGDFNDIMRKDRSGFRPPNSKDMGTTDSGPYFEKGGSHGLFGNSTAQSRGLHTPVQPLNSSPSLRAQVPPQFISPQVSASMLKQFPNSGLSPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQLLQNQRKISQAVRQQQEQQLARMVSALQQQQQQQQRQPGMKHSPSHPVGPKPHLDNMVPNALNVGLPDLQTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEANMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTATSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLPRPPPGLTNPKPSSPWSSTAPRSVRGWGTQDSRLASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATDDEVSRFLAQAQPPTPAATPSAPAAGWQSLETGQNQSDPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGASLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI DIP protein Q9UPQ9-2 original sequence version: 49 4CF58AF70B8973B3 ago2_human Protein argonaute-2 AGO2 Argonaute RISC catalytic component 2 EIF2C2 Eukaryotic translation initiation factor 2C 2 PAZ Piwi domain protein Protein slicer protein protein human Homo sapiens Human MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA DIP protein Q9UKV8 original sequence version: 1(def) DIP protein Q9UKV8 original sequence version: 44 DIP protein Q9UKV8 original sequence version: 60 DIP protein Q9UKV8 original sequence version: 62 DIP protein Q9UKV8 original sequence version: 89 DIP protein Q9UKV8 original sequence version: 85 DIP protein Q9UKV8 original sequence version: 87 DIP protein Q9UKV8 original sequence version: 99 DIP protein Q9UKV8 original sequence version: 117 DIP protein Q9UKV8-1 original sequence version: 117 DIP protein Q9UKV8 original sequence version: 145 DIP protein Q9UKV8 original sequence version: 108 5C8552C43FC81345 piwi_arcfu Piwi protein AF_1318 AfAgo PIWI/MID domain protein AF_1318 AfPiwi protein protein arcfu Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) MMEYKIVENGLTYRIGNGASVPISNTGELIKGLRNYGPYEVPSLKYNQIALIHNNQFSSLINQLKSQISSKIDEVWHIHNINISEFIYDSPHFDSIKSQVDNAIDTGVDGIMLVLPEYNTPLYYKLKSYLINSIPSQFMRYDILSNRNLTFYVDNLLVQFVSKLGGKPWILNVDPEKGSDIIIGTGATRIDNVNLFCFAMVFKKDGTMLWNEISPIVTSSEYLTYLKSTIKKVVYGFKKSNPDWDVEKLTLHVSGKRPKMKDGETKILKETVEELKKQEMVSRDVKYAILHLNETHPFWVMGDPNNRFHPYEGTKVKLSSKRYLLTLLQPYLKRNGLEMVTPIKPLSVEIVSDNWTSEEYYHNVHEILDEIYYLSKMNWRGFRSRNLPVTVNYPKLVAGIIANVNRYGGYPINPEGNRSLQTNPWFL F925081F3AFB47CD ago1_schpo Protein argonaute Eukaryotic translation initiation factor 2C 2-like protein ago1 PAZ Piwi domain protein ago1 Cell cycle control protein ago1 RNA interference pathway protein ago1 ago1 csp9 SPCC736.11 Protein slicer protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSYKPSSEIALRPGYGGLGKQITLKANFFQIISLPNETINQYHVIVGDGSRVPRKQSQLIWNSKEVKQYFGSSWMNSVYDGRSMCWSKGDIADGTIKVNIGSESHPREIEFSIQKSSKINLHTLSQFVNSKYSSDPQVLSSIMFLDLLLKKKPSETLFGFMHSFFTGENGVSLGGGVEAWKGFYQSIRPNQGFMSVNVDISSSAFWRNDSLLQILMEYTDCSNVRDLTRFDLKRLSRKFRFLKVTCQHRNNVGTDLANRVYSIEGFSSKSASDSFFVRRLNGEEQKISVAEYFLENHNVRLQYPNLPCILVKNGAMLPIEFCFVVKGQRYTAKLNSDQTANMIRFAVQRPFERVQQIDDFVHQMDWDTDPYLTQYGMKIQKKMLEVPARVLETPSIRYGGDCIERPVSGRWNLRGKRFLDPPRAPIRSWAVMCFTSTRRLPMRGIENFLQTYVQTLTSLGINFVMKKPPVLYADIRGSVEELCITLYKKAEQVGNAPPDYLFFILDKNSPEPYGSIKRVCNTMLGVPSQCAISKHILQSKPQYCANLGMKINVKVGGINCSLIPKSNPLGNVPTLILGGDVYHPGVGATGVSIASIVASVDLNGCKYTAVSRSQPRHQEVIEGMKDIVVYLLQGFRAMTKQQPQRIIYFRDGTSEGQFLSVINDELSQIKEACHSLSPKYNPKILVCTTQKRHHARFFIKNKSDGDRNGNPLPGTIIEKHVTHPYQYDFYLISHPSLQGVSVPVHYTVLHDEIQMPPDQFQTLCYNLCYVYARATSAVSLVPPVYYAHLVSNLARYQDVTADDTFVETSEASMDQEVKPLLALSSKLKTKMWYM F5CE397BF590D9E4 F5CE397BF590D9E4 tnrc6b-ago1-6 4 7 unspecified role unspecified role prey prey 515-816binding region 515-816binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait 198-275 binding region 198-275 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction tnrc6b-ago2-2 6 10 unspecified role unspecified role prey prey 509-810 binding region 509-810 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q9UKV8:p.Tyr529Ala Y529A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y529A mutation'. Wed May 02 16:45:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr529ala' Tue Jun 30 17:05:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr529Ala' Q9UKV8:p.Lys533Ala K533A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K533A mutation'. Wed May 02 15:50:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys533ala' Tue Jun 30 16:57:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys533Ala' Q9UKV8:p.Gln545Ala E545A mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 17:42:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln545ala' Tue Jun 30 15:47:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln545Ala' Q9UKV8:p.Arg583Ala R583A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R583A mutation'. Wed May 02 19:19:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg583ala' Tue Jun 30 18:28:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg583Ala' Q9UKV8:p.Ile592Ala I592A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I592A mutation'. Wed May 02 20:03:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile592ala' Tue Jun 30 16:38:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile592Ala' Q9UKV8:p.Tyr625Ala Y625A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y625A mutation'. Wed May 02 17:40:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr625ala' Tue Jun 30 18:07:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr625Ala' Q9UKV8:p.Arg647Ala R647A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R647A mutation'. Wed May 02 18:23:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg647ala' Tue Jun 30 17:50:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg647Ala' Q9UKV8:p.Phe653Ala F653A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F653A mutation'. Wed May 02 19:45:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe653ala' Tue Jun 30 18:46:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe653Ala' Q9UKV8:p.Val797Ala V797A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V797A mutation'. Wed May 02 19:05:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val797ala' Tue Jun 30 16:08:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val797Ala' Q9UKV8:p.Arg812Ala R812A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R812A mutation'. Wed May 02 17:50:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg812ala' Tue Jun 30 15:51:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg812Ala' Q9UKV8:p.Phe587Ala F587A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F587A mutation'. Wed May 02 15:29:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe587ala' Tue Jun 30 19:51:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe587Ala' Q9UKV8:p.Val615Ala V615A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V615A mutation'. Wed May 02 18:32:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val615ala' Tue Jun 30 15:48:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val615Ala' Q9UKV8:p.Tyr749Ala Y749A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y749A mutation'. Wed May 02 18:44:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr749ala' Tue Jun 30 20:01:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr749Ala' Q9UKV8:p.Tyr784Ala Y784A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y784A mutation'. Wed May 02 16:42:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr784ala' Tue Jun 30 19:37:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr784Ala' Q9UKV8:p.Tyr805Ala Y805A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y805A mutation'. Wed May 02 17:25:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr805ala' Tue Jun 30 17:23:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr805Ala' Q9UKV8:p.Phe858Ala F858A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F858A mutation'. Wed May 02 19:06:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe858ala' Tue Jun 30 15:41:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe858Ala' in vitro In vitro 9 unspecified role unspecified role bait bait 198-219 binding region 198-219 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9UPQ9-2:p.Trp205Phe W205F mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W F This feature has been corrected as a result of our quality control procedures. The original label was 'W205F mutation'. Wed May 02 17:18:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp205phe' Tue Jun 30 19:24:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp205Phe' Q9UPQ9-2:p.Trp215Phe W215F mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W F This feature has been corrected as a result of our quality control procedures. The original label was 'W215F mutation'. Wed May 02 20:00:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp215phe' Tue Jun 30 17:58:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp215Phe' Q9UPQ9-2:p.[Trp205Ala;Trp215Ala] W205A,W215A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W205A,W215A mutation'. Wed May 02 18:12:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp205ala,trp215ala' Tue Jun 30 18:34:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp205Ala;Trp215Ala]' ecolx Escherichia coli direct interaction direct interaction tas3-ago1-1 5 8 unspecified role unspecified role bait bait 248-308 binding region 248-308 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey 381-834 binding region 381-834 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction tnrc6b-afpiwi-1 3 11 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait 198-219 binding region 198-219 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction tnrc6b-ago1-7 1 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait 198-275 binding region 198-275 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association tnrc6b-ago2-5 2 10 unspecified role unspecified role prey prey HA Tag HA Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 9 unspecified role unspecified role bait bait GFP Tag GFP Tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 198-275 binding region 198-275 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association