DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255072X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255066X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255071X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255069X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255064X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255061X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255073X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255065X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255063X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255067X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255068X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255062X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. Nature structural & molecular biology 2007 imex curation Chen HT., Warfield L., Hahn S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Chen HT., Warfield L., Hahn S. DIP original experiment: DIP-255070X Nature structural & molecular biology 2007 IMEx The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex. t2fb_yeast Transcription initiation factor IIF subunit beta YGR005C TFG2 TFIIF-beta TFIIF medium subunit Transcription factor G 54 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSGSAGAPALSNNSTNSVAKEKSGNISGDEYLSQEEEVFDGNDIENNETKVYEESLDLDLERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIRINKDGSKITLLLNENDNDSIPHEYDLELTKKVVENEYVFTEQNLKKYQQRKKELEADPEKQRQAYLKKQEREEELKKKQQQQKRRNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIVGTVCHECQVMPSMNDPNYHKIVEQRRNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNSNFLKVGREKAKSNIKSIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLDKVATLVKKGPYAFKYTLRPEYKKLKEEERKATLGELADEQTGSAGDNAQGDAEADLEDEIEMEDVV DDDA895446BBF26F DDDA895446BBF26F tf2b_yeast Transcription initiation factor IIB SUA7 YPR086W P9513.4 General transcription factor TFIIB Transcription factor E protein protein yeast Saccharomyces cerevisiae Baker's yeast MMTRESIDKRAGRRGPNLNIVLTCPECKVYPPKIVERFSEGDVVCALCGLVLSDKLVDTRSEWRTFSNDDHNGDDPSRVGEASNPLLDGNNLSTRIGKGETTDMRFTKELNKAQGKNVMDKKDNEVQAAFAKITMLCDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGCRRAEVARTFKEIQSLIHVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIPRFCSHLGLPMQVTTSAEYTAKKCKEIKEIAGKSPITIAVVSIYLNILLFQIPITAAKVGQTLQVTEGTIKSGYKILYEHRDKLVDPQLIANGVVSLDNLPGVEKK 8F1F6D24602436E2 8F1F6D24602436E2 rpb1_yeast DNA-directed RNA polymerase II subunit RPB1 RPO21 RPB220 RPB1 SUA8 YDL140C D2150 DNA-directed RNA polymerase III largest subunit RNA polymerase II subunit B220 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVGQQYSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIKDSKKRFAAIWTLCKTKMVCETDVPSEDDPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEHTTLKSVTIASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFDQQSPWLLRLELDRAAMNDKDLTMGQVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVVRPKSLDAETEAEEDHMLKKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGAFDVMIDEESLVKYMPEQKITEIEDGQDGGVTPYSNESGLVNADLDVKDELMFSPLVDSGSNDAMAGGFTAYGGADYGEATSPFGAYGEAPTSPGFGVSSPGFSPTSPTYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPGYSPGSPAYSPKQDEQKHNENENSR A45C1360FF99F968 A45C1360FF99F968 t2eb_yeast Transcription initiation factor IIE subunit beta YKR062W TFA2 Transcription factor A small subunit Factor A 43 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKNRDPLLANLNAFKSKVKSAPVIAPAKVGQKKTNDTVITIDGNTRKRTASERAQENTLNSAKNPVLVDIKKEAGSNSSNAISLDDDDDDEDFGSSPSKKVRPGSIAAAALQANQTDISKSHDSSKLLWATEYIQKKGKPVLVNELLDYLSMKKDDKVIELLKKLDRIEFDPKKGTFKYLSTYDVHSPSELLKLLRSQVTFKGISCKDLKDGWPQCDETINQLEEDSKILVLRTKKDKTPRYVWYNSGGNLKCIDEEFVKMWENVQLPQFAELPRKLQDLGLKPASVDPATIKRQTKRVEVKKKRQRKGKITNTHMTGILKDYSHRV 8751614A7237D3E1 8751614A7237D3E1 smtfg1 Transcription initiation factor IIF subunit alpha protein protein sacmi Saccharomyces mikatae Yeast MSRRNPPGSRNGGGPTNASPFIKRDRMRRNFLRMRMGQNSSNSSSSSVLNENGSRGSLVKKDDPEYAEERERMLLQIGVEADAGRSNVKVKDEDPNEYNEFPLRAIPKEDLENMRTHLLKFQSKKKINPVTDFHLPVRLHRKDTRNLQFQLTRAEIVQRQREISEYKKKAEQERSTPIPGGMNKPGTVPLNNTARDGSQTPVIDSAIKDNATNGVNSMTATATGSSVPPASPNPASAVENNAVVIGSSGVSGTVATDPANGKLLVTKLEDAGPAEDPTKVGMVKYDGKEVANEPEFEEGTMDPLADVAPDGGGRAKRGNMRRKTRQLKVLDENAKKLRFEEFYPWVMEDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYKFTARNKYATLTIDEAEKRMDKKSGEVPRWLMKHLDNIGTTTTRYDRTRRKLKAVADQQAMDEDDRDDNSEVELDYDEEFADDEEAPIIDGNEQENKESEQRIKKEMLQANAMGLRDEEAPSGNEEDELFGEKKIDEDGEKIKKALQKTELAALYSSDEDEINPYLSESDIENKENEPSVKKEEDIDSLSKSKKSSPKKQQKKTANAHVHKEPNLRVKSIKDCVIILKGDKKVLKSFPEGEWNPHAAKIKDISGNESNAVSAPIKKEKPLESIVVQEKETPAPLITEQDIIDAIGDGKVNVKEFGKSIRRRYPGAENKKLMFAIVKKLCRKVDNEHMELKKEQVNL rpb2_yeast DNA-directed RNA polymerase II subunit RPB2 YOR151C RPB2 RPB150 RPO22 DNA-directed RNA polymerase II 140 kDa polypeptide B150 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLANSEKYYDEDPYGFEDESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTLILEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARLRNLTYSSGLFVDVKKRTYEAIDVPGRELKYELIAEESEDDSESGKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQDRETALDFIGRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVEEAHDFNMKLAINAKTITSGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLEPAEANEENDLDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVSGEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDGLKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEASDAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRLYTDRSRDF BABD03212C0A583E BABD03212C0A583E t2ea_yeast Transcription initiation factor IIE subunit alpha YKL028W TFA1 Transcription factor A large subunit Factor A 66 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MDRPIDDIVKNLLKFVVRGFYGGSFVLVLDAILFHSVLAEDDLKQLLSINKTELGPLIARLRSDRLISIHKQREYPPNSKSVERVYYYVKYPHAIDAIKWKVHQVVQRLKDDLDKNSEPNGYMCPICLTKYTQLEAVQLLNFDRTEFLCSLCDEPLVEDDSGKKNKEKQDKLNRLMDQIQPIIDSLKKIDDSRIEENTFEIALARLIPPQNQSHAAYTYNPKKGSTMFRPGDSAPLPNLMGTALGNDSSRRAGANSQATLHINITTASDEVAQRELQERQAEEKRKQNAVPEWHKQSTIGKTALGRLDNEEEFDPVVTASAMDSINPDNEPAQETSYQNNRTLTEQEMEERENEKTLNDYYAALAKKQAKLNKEEEEEEEEEEDEEEEEEEEMEDVMDDNDETARENALEDEFEDVTDTAGTAKTESNTSNDVKQESINDKTEDAVNATATASGPSANAKPNDGDDDDDDDDDEMDIEFEDV 3E789DFC4247EF8A 3E789DFC4247EF8A rpb2-tfg2-1 4 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association smtfg1-tfg2-1 7 14 unspecified role unspecified role prey prey Region 1-230 Region 1-230 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 18 unspecified role unspecified role bait bait Region 1-424 Region 1-424 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal in vitro In vitro direct interaction direct interaction rpb1-tfiib-1 6 15 unspecified role unspecified role unspecified role unspecified role CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb2-smtfg1-1 8 18 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb1-rpb2-2 12 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb1-tfa1-1 13 20 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb2-smtfg1-2 2 18 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb1-tfa2-1 3 17 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb1-rpb2-3 9 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb2-smtfg1-3 10 18 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb2-smtfg1-4 11 18 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rpb2-rpb1-1 1 14 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 19 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association rpb2-tfiib-1 5 19 unspecified role unspecified role unspecified role unspecified role Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 15 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association