DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nature structural & molecular biology 2007 imex curation Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66853X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nature structural & molecular biology 2007 imex curation Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66854X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nature structural & molecular biology 2007 imex curation Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66849X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nature structural & molecular biology 2007 imex curation Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66852X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nature structural & molecular biology 2007 imex curation Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66851X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. Nature structural & molecular biology 2007 imex curation Corbett KD., Benedetti P., Berger JM. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Corbett KD., Benedetti P., Berger JM. DIP original experiment: DIP-66850X Nature structural & molecular biology 2007 IMEx Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI. top6b_metma Type 2 DNA topoisomerase 6 subunit B top6B Type II DNA topoisomerase VI subunit B MM_2417 protein protein metma Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) METPIAEELAKKQKSISVAEFFEKNRQILGFDSAPRSLITTVKEAVDNALDACEEAGILPDILVQVERTGPDYVTVIIEDNGPGIVREQIPKVFAKLLYGSRFHALKQSRGQQGIGISAAVLYAQMTAGRHTKILSKTSPTAPAHYYELMINTSTNEPDILVDEVRDWFRPHGTQIELEMRAAYVKGRRQSIYEYLKATAIVNPHARITLIDPDGNEEVFERATDKMPEPAEEILPHPEGIELGTLMKMLHYTERQKLAPFLRYSFCKIGLLTAEEICKAAGLDPEIDPHALGRHEARKLIEAFEKVKIMAPPTDCLSPIGEDLIYRGLEKETTVDFIATSTRKPAVYSGNPFVVEVGMAYGGNLPKEEKISIMRFANRVPLLYQQGGCVTTHAVEDIKWKQYGLNQPGGGIPVGPVILLIHVASINVPFTSESKDAIADIPVIKEEIDLAIKEVARKLKHYLSKQSNLKKRREKEIIITKVLPKLAAKVAHVLEKDVPDINPVVAKIMGNLLVHRVIKNNGDGTVDVAIKVKNFGTSAYSFRVHEMLPCKVSGAKPEPKVVTMGNDYDYVWDISASAGSSKVLSYKIESASEEELQKLPQLIVEGIEEELVTGAKAFKGV 7D2FEC4F2FA68AD6 7D2FEC4F2FA68AD6 top6a_sacsh Type 2 DNA topoisomerase 6 subunit A top6A Type II DNA topoisomerase VI subunit A protein protein sulsh Sulfolobus shibatae MSSEFISKVDKEARRKAANILRDKFLNLVEQLKKGEPLVMEIPMRTLSNAIYDEKRKLLLLGEKKLRRNFLDLNEAKRFMQTVLMASIIYDALVSDEYPTIRDLYYRGKHSLLLKSIEGNKIVSEENTWDEQKESDSVIVDIEVFTSLLREEMLILSKEKGKVVGNLRIRSGNDVIDLSKTGHGAYAIEPTPDLIDFIDVDAEFVLVVEKDAVFQQLHRAGFWKQYKSILITSAGQPDRATRRFVRRLNEELKLPVYILTDADPYGWYIFSVFRIGSISLSYESERLATPDAKFLGVSMGDIFGNSRKKPYLSEAERKNYIIKAKDADIKRAEEIKNYEWFKTKAWQEEINTFLQRKAKLEIEAMASKGLKFLAFQYIPEKITNKDYIA DIP protein O05208 original sequence version: 48 DIP protein O05208 original sequence version: 50 6F617CB8231431B7 top6a_metma Type 2 DNA topoisomerase 6 subunit A top6A Type II DNA topoisomerase VI subunit A MM_2418 protein protein metma Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) MEGEKGSKTRKGDALAREKLLEIAEKIYNQFEEEVVPSVSLPSRTKANLEYSDESDVWVYGDRESERSAKTVKGAFQLLKTTYATDFLINEHLARNRGSTLRELYYISEGWDYAKFKEQGESDRLIEDLEILTSLQREYFHMRPEEDGATMFGPIEITEQTKRGERNIHCQKDVGEGGYQIPFNVENIEFQKHDASMIIAIETGGMYARLMENGFDEAYNAILVHLKGQPARSTRRIIKRMNEELGIPVAVFTDGDPWSYRIYASVAYGAIKSAHLSEFMATPAAKFLGLQPSDIVEYELSTDKLTEQDVSALRSELSDPRFESDYWKEQIQLQLDIGKKAEQQAFAGKGLDFVTEVYLPNRLKEMGMI CF7FA9AC6F4936CA CF7FA9AC6F4936CA top6b_sacsh Type 2 DNA topoisomerase 6 subunit B top6B Type II DNA topoisomerase VI subunit B protein protein sulsh Sulfolobus shibatae MSAKEKFTSLSPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDVHGILPNIKITIDLIDDARQIYKVNVVDNGIGIPPQEVPNAFGRVLYSSKYVNRQTRGMYGLGVKAAVLYSQMHQDKPIEIETSPVNSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIPGDWPKAKSRIYEYIKRTYIITPYAEFIFKDPEGNVTYYPRLTNKIPKPPQEVKPHPYGVDREEIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAGLKPNKKVKNLTEEEITRLVETFKKDEDFRSPSADSLSVIGEDLIELGLKKIFNPDFAASITRKPKAYQGHPFIVEAGVAFGGSIPVGEEPIVLRYANKIPLIYDEKSDVIWKVVEELDWKRYGIESDQYQMVVMVHLCSTKIPYKSAGKESIAEVENIEKEIKNALMEVARKLKQYLSEKRKEQEAKKKLLAYLKYIPEVSRSLATFLASGNKELVSKYQNEISEGLFKLISKKLDLINIEEYRKVYRVDSE DIP protein O05207 original sequence version: 72 DIP protein O05207 original sequence version: 53 DIP protein O05207 original sequence version: 58 772F221FCD28441A top6b-top6a-1 6 7 unspecified role unspecified role bait bait His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range 1-511 binding region 1-511 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction top6a-top6b-1 3 7 unspecified role unspecified role neutral component neutral component 1-511 binding region 1-511 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction top6a-top6b-2 5 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction top6a-top6b-3 1 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction top6b-3 2 10 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction top6b-top6a-2 4 8 unspecified role unspecified role prey prey ecolx Escherichia coli 10 unspecified role unspecified role bait bait His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction