DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions human Homo sapiens Human comigration in sds comigration in sds page comigration in sds ms participant Identification by mass spectrometry ms participant Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255307X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255305X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255308X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255309X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255311X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255310X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions human Homo sapiens Human pull down pull down predetermined predetermined participant predetermined Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255304X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 2007 imex curation Jin J., Li X., Gygi SP., Harper JW. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Jin J., Li X., Gygi SP., Harper JW. DIP original experiment: DIP-255306X Nature 2007 IMEx Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. isg15_human Ubiquitin-like protein ISG15 ISG15 G1P2 UCRP Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Ubiquitin cross-reactive protein protein protein human Homo sapiens Human MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS DIP protein P05161 original sequence version: 130 DIP protein P05161 original sequence version: 142 DIP protein P05161 original sequence version: 155 DIP protein P05161 original sequence version: 96 DIP protein P05161 original sequence version: 141 DIP protein P05161 original sequence version: 168 DIP protein P05161 original sequence version: 150 B6858A15AB0FFFDE ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG a0avt1-1 Ubiquitin-like modifier-activating enzyme 6 UBA6 MOP4 UBE1L2 Monocyte protein 4 Ubiquitin-activating enzyme E1-like protein 2 protein protein human Homo sapiens Human MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD DIP protein A0AVT1-1 original sequence version: 75 uba1_human Ubiquitin-like modifier-activating enzyme 1 Ubiquitin-activating enzyme E1 Protein A1S9 UBA1 A1S9T UBE1 protein protein human Homo sapiens Human MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR DIP protein P22314 original sequence version: 157 DIP protein P22314 original sequence version: 152 4B413AAA75FAA562 uba7_human Ubiquitin-like modifier-activating enzyme 7 UBE1L UBE2 UBA7 Ubiquitin-activating enzyme E1 homolog D8 protein protein human Homo sapiens Human MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL DIP protein P41226 original sequence version: 130 A01E1106D81778EB ub-uba1-1 7 10 unspecified role unspecified role bait bait human Homo sapiens Human 11 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 12 unspecified role unspecified role prey prey human Homo sapiens Human association association ub-uba6-1 3 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ub-uba6-2 8 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined A0AVT1-1:p.Cys625Ala C625A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C625A'. Wed May 02 16:03:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys625ala' Tue Jun 30 16:17:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys625Ala' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm covalent binding covalent binding isg15-uba7-5 5 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ub-uba1-2 4 12 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction uba6-ub-1 2 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined A0AVT1-1:p.Cys625Ala C625A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C625A'. Wed May 02 17:22:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys625ala' Tue Jun 30 16:45:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys625Ala' human Homo sapiens Human physical association physical association ub-uba6-3 1 10 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 11 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human direct interaction direct interaction ub-uba1-3 6 12 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction