DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Eakin CM., Maccoss MJ., Finney GL., Klevit RE. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Eakin CM., Maccoss MJ., Finney GL., Klevit RE. DIP original experiment: DIP-261291X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Eakin CM., Maccoss MJ., Finney GL., Klevit RE. Only protein-protein interactions in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Eakin CM., Maccoss MJ., Finney GL., Klevit RE. DIP original experiment: DIP-261290X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase. p03372-1 Estrogen receptor ESR1 ESR NR3A1 ER-alpha Estradiol receptor Nuclear receptor subfamily 3 group A member 1 ER66 Long hER-alpha66 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372-1 original sequence version: 214 DIP protein P03372-1 original sequence version: 139 DIP protein P03372-1 original sequence version: 204 DIP protein P03372-1 original sequence version: 171 DIP protein P03372-1 original sequence version: 179 DIP protein P03372-1 original sequence version: 206 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG esr1-ub-1 2 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role K302 K302 ubiquitinylated lysine ubiquitinylated lysine modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain Region 302-552 Region 302-552 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli covalent binding covalent binding esr1-ub-2 1 4 unspecified role unspecified role unspecified role unspecified role T7-tag T7-tag t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role Region 302-552 Region 302-552 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction