IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex. Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex. Archives of biochemistry and biophysics 2007 imex curation Hsieh EJ., Gin P., Gulmezian M., Tran UC., Saiki R., Marbois BN., Clarke CF. Purified mitochondria were solubilized with digitonin and subject to 100,000 × g centrifugation. then to 2-dimensional Blue Native gel electrophoresis. cathy@chem.ucla.edu 14-Jul-2017: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Hsieh EJ., Gin P., Gulmezian M., Tran UC., Saiki R., Marbois BN., Clarke CF. Purified mitochondria were solubilized with digitonin and subject to 100,000 × g centrifugation. then to 2-dimensional Blue Native gel electrophoresis. cathy@chem.ucla.edu Accepted 2017-JUN-23 AT 09:56 BST AT 09:56 BST by MGT Archives of biochemistry and biophysics 2007 imex curation Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex. Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex. Archives of biochemistry and biophysics 2007 imex curation Hsieh EJ., Gin P., Gulmezian M., Tran UC., Saiki R., Marbois BN., Clarke CF. Purified mitochondria were solubilized with digitonin and subject to 100,000 × g centrifugation. then to 2-dimensional Blue Native gel electrophoresis. cathy@chem.ucla.edu 14-Jul-2017: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hsieh EJ., Gin P., Gulmezian M., Tran UC., Saiki R., Marbois BN., Clarke CF. Purified mitochondria were solubilized with digitonin and subject to 100,000 × g centrifugation. then to 2-dimensional Blue Native gel electrophoresis. cathy@chem.ucla.edu Accepted 2017-JUN-23 AT 09:56 BST AT 09:56 BST by MGT Archives of biochemistry and biophysics 2007 imex curation coq8_yeast Atypical kinase COQ8, mitochondrial COQ8 ABC1 Activity of bc1 complex protein 1 Coenzyme Q protein 8 Ubiquinone biosynthesis protein COQ8 YGL119W protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTNMVKLRNLRRLYCSSRLLRTIQNGRISSVSSISLSKKYTTKSAKEGEENVERKHEEEKKDTLKSSSVPTSRISRLFHYGSLAAGVGMNAAAKGISEVAKGNSPTWKSLILSDSNIDRITNKFSKMRGVALKIGQLLSFQDEKVLPKELYEILSRVQNSANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQYPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKFEALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCLEEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDRKGAYEMSVQLGYLTGLESQSMKDAHVDSVLTLGEPFRGDVDKSFDFKDQTVSDRIRGNIGLMLNERLCPPPEETYSLHRKFSGIFLLCARMGASVHCAKLFKEIFAYKV C91D1EEE5A7ACC9C coq4_yeast Ubiquinone biosynthesis protein COQ4, mitochondrial COQ4 YDR204W YD8142.01 Coenzyme Q biosynthesis protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRLSLLRSTATLPVKCQRRGLILPAAAMYTLGSLIFGKEARLADAMERGELHNKNVDYAKEAEERTELRIRALANTRPMEPRYNGHVPLHRYEKLLLFAISGWNSFFHPEDGYNIVQLGEATALPVFLENLKQTMLSDSSGRRILKEQPNITTEILHMDKLAKLPHNTFGYVYYQWLKRENVSPDTRAPVKFIDDPMHAYIFKRYRQCHDFYHAITNMPIIIEGEITIKALEGANLGVPMAILGGILAPLRLKKVQRKRLYNIYLPWAVRTGLSCKPLINVYWEEMLEKDVTALRKELKITLPPDLRTMRKERAALRKEIDAKYNSQKRATTPA C8AFBFA249007901 coq6_yeast Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial COQ6 Ubiquinone C4-deaminase G9165 YGR255C protein protein yeast Saccharomyces cerevisiae Baker's yeast MFFSKVMLTRRILVRGLATAKSSAPKLTDVLIVGGGPAGLTLAASIKNSPQLKDLKTTLVDMVDLKDKLSDFYNSPPDYFTNRIVSVTPRSIHFLENNAGATLMHDRIQSYDGLYVTDGCSKATLDLARDSMLCMIEIINIQASLYNRISQYDSKKDSIDIIDNTKVVNIKHSDPNDPLSWPLVTLSNGEVYKTRLLVGADGFNSPTRRFSQIPSRGWMYNAYGVVASMKLEYPPFKLRGWQRFLPTGPIAHLPMPENNATLVWSSSERLSRLLLSLPPESFTALINAAFVLEDADMNYYYRTLEDGSMDTDKLIEDIKFRTEEIYATLKDESDIDEIYPPRVVSIIDKTRARFPLKLTHADRYCTDRVALVGDAAHTTHPLAGQGLNMGQTDVHGLVYALEKAMERGLDIGSSLSLEPFWAERYPSNNVLLGMADKLFKLYHTNFPPVVALRTFGLNLTNKIGPVKNMIIDTLGGNEK B6D4286545CCE695 coq7_yeast 5-demethoxyubiquinone hydroxylase, mitochondrial COQ7 CAT5 Catabolite repression protein 5 Ubiquinone biosynthesis monooxygenase COQ7 O3284 YOR3284C YOR125C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSRVSVFKPASRGFSVLSSLKITEHTSAKHTEKPEHAPKCQNLSDAQAAFLDRVIRVDQAGELGADYIYAGQYFVLAHRYPHLKPVLKHIWDQEIHHHNTFNNLQLKRRVRPSLLTPLWKAGAFAMGAGTALISPEAAMACTEAVETVIGGHYNGQLRNLANQFNLERTDGTKGPSEEIKSLTSTIQQFRDDELEHLDTAIKHDSYMAVPYTVITEGIKTICRVAIWSAERI 2686F44C5F17EFE5 2686F44C5F17EFE5 coq5_yeast 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial COQ5 DBI56 YML110C YM8339.09C Ubiquinone biosynthesis methyltransferase COQ5 2-hexaprenyl-6-methoxy-1,4-benzoquinol methyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLISSRIVRSSLVNVPLRLSRCFTQAHRACKEEEVNSPLSSAAEQPEQKYTHFGSKTVLKSTKQKLVGDVFSSVANRYDLMNDVMSLGIHRLWKDHFINKLDAGKRPNSTTPLNFIDVAGGSGDIAFGLLDHAESKFGDTESTMDIVDINPDMLKEGEKRAMEQGKYFKDPRVRFLVSNGEKLEEIDSDSKDIYTVSFGIRNFTDIQKGLNTAYRVLKPGGIFYCLEFSKIENPLMDFAYQQWAKVLPVMGSMIANDYDSYQYLVESIERFPDQETFKSMIEKAGFKSAGYESLTFGICAIHWGIKV C8951F9C8FFF8F41 coq9_yeast Ubiquinone biosynthesis protein COQ9, mitochondrial YLR201C COQ9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLCRNTARTGCKFFRLYHSNPIEHVKPIHIKPLTYGKESPQYKVLSLALQKFVPEHGFSERSIVESLNELGYPSSMISSIGAPNSPSFFHSSTAVMELIKFQLVDKRYRLTEGINPDVTPQYKLPSLEHLLLKRLEMDKPIGGHLSELMSQLAIPSAFLFETAIPELHRLSDDMIYFSNEKDHHDSAWYAKRLAVSSTYIGSKLFMAQDKSHNYKETFTFAKDKLHRVMRLGEYYNNTEEFAWYTLMSTVNLIKSQLVRG 7B92318973210D67 coq3_yeast Ubiquinone biosynthesis O-methyltransferase, mitochondrial COQ3 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase 3-demethylubiquinol 3-O-methyltransferase 3-demethylubiquinone-6 3-O-methyltransferase Hexaprenyldihydroxybenzoate methyltransferase Polyprenyldihydroxybenzoate methyltransferase HRF316 YOL096C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLLRSRFLKVIHVRKQLSACSRFAIQTQTRCKSTDASEDEVKHFQELAPTWWDTDGSQRILHKMNLTRLDFVQRTVRNQVKIQNPEIFVPGFNYKEFLPEYVCDNIQREMQESIETNLDKRPEVSVLDVGCGGGILSESLARLKWVKNVQGIDLTRDCIMVAKEHAKKDPMLEGKINYECKALEDVTGQFDIITCMEMLEHVDMPSEILRHCWSRLNPEKGILFLSTINRDLISWFTTIFMGENVLKIVPKGTHHLSKYINSKEILAWFNDNYSGQFRLLDLKGTMYLPYQGWVEHDCSDVGNYFMAIQRLN F0D973ADE4C2D6FD coq3-coq4-1 1 8 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 4B coq3-coq4-2 2 8 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Fig. 5