DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Raskin DM., Judson N., Mekalanos JJ. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Raskin DM., Judson N., Mekalanos JJ. DIP original experiment: DIP-261189X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Raskin DM., Judson N., Mekalanos JJ. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Raskin DM., Judson N., Mekalanos JJ. DIP original experiment: DIP-261188X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. spot_vibch Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase spoT Penta-phosphate guanosine-3'-pyrophosphohydrolase VC_2710 protein protein vibch Vibrio cholerae MYLFDSLKDVAQEYLTEPQIEALRQSYVVARDAHEGQTRSSGEPYIIHPVAVARILAEMRLDLETLQAALLHDVIEDCDVTKEDLDAHFGSSVAELVDGVSKLDKLKFRDRKEAQAENFRKMVLAMVQDIRVILIKLADRTPNMRTLGALRPDKKRRIARETLEIYAPLAHRLGIHNIKTELEELGFEALYPNRYRVLKEVVKAARGNRKEMIQRIHSEIEGRLQEVGLPARVVGREKNLFSIYNKMKTKEQRFHTIMDIYAFRIVVDTADTCYRVLGQVHSLYKPRPARMKDYIAVPKANGYQSLHTSMVGPHGVPVEVQIRTEDMDQMADKGVAAHWSYKANSERGGTTAQIKAQRWMQSLLELQQSAGNSFEFIENVKSDLFPDEIYVFTPKGRIVELPMGATAVDFAYAVHTDIGNTCVGARVDRTPYPLSQSLKSGQTVEIISAPGARPNAAWLNYVVTSRARTKIRQVLKTMRREDSITLGRRLLNHALGEHSVNEIAPENISKVLSDLKIASMDDLLAAIGLGELMSIVIARRLLGNADELTEPSKSGGNKNKLPIRGAEGILLTFANCCHPIPDDHIIAHVSPGRGLVVHRETCPNVRGYQKEPDKYMAVEWTKDYDQEFITELKVDMHNRQGALAELTNVISKTGSNIHGLSTEERDGRLYTVTVLLTTKDRVHLAGIMRKIRTMPHALKVRRRKN DIP protein Q9KNM2 original sequence version: 81 obg_vibch GTPase Obg/CgtA cgtA obg VC_0437 CgtA GTP-binding protein Obg protein protein vibch Vibrio cholerae MKFVDEAVIKVQAGDGGNGVVSFWREKFVTNGGPDGGDGGDGGDVYMVADENLNTLIDYRFQRFYEAERGKNGGGGNCTGKSGKDKELRVPVGTRAVDIHTNEIIGEVAEHGKKVMIAKGGWHGLGNARFKSSVNRSPRQKTLGTKGELRDIRLELLLLADVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVSVLPEKSFVVADIPGLIEGAAEGAGLGIRFLKHLERCRVLLHMIDIMPADQSDPAHNALTIIDELEQYSEKLAKKPRWLVFNKVDLMSEEEADEIIQNIIDALAWEGDYFKISAANRQGTKELCMKLAEFMDTLPREAEEKTEAEKVDFTWDYNHKDGLAGREVITEDDDDWDDWDDEEDDGHVIYVRD DIP protein Q9KUS8 original sequence version: 75 cgta-cgta-1 2 4 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association cgta-spot-1 1 3 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association