IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ 70-kDa heat shock proteins: specific interactions with HLA-DR molecules and their peptide fragments. 70-kDa heat shock proteins: specific interactions with HLA-DR molecules and their peptide fragments. European journal of immunology 2007 imex curation Haug M., Schepp CP., Kalbacher H., Dannecker GE., Holzer U. ursula.holzer@med.uni‐tuebingen.de 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Haug M., Schepp CP., Kalbacher H., Dannecker GE., Holzer U. Interactions involving HSC70(Hsc73) have not been included as the main text and Methods sections are ambiguous as to the origins of this protein - human or bovine? I left peptide biotin tags as "?-?" as text is unclear, stating "some peptides were specifically biotinylated at their N terminus using ..." So does this mean that ALL biotinylated peptides have an N-terminal tag, or only some? (Rohrer -2014 publication clearly states N-terminal tag.) Human HSP70 is stated to be the "dimer" in Enzo catalogue. DELFIA: Dissociation-enhanced lanthanide fluorescent immunoassay. Hsp70/Hsc70/DnaK and biotinylated peptide/protein mixtures were either added directly to plates, or plates coated with anti-Hsp70/Hsc70/DnaK monoclonal antibody. After washing biotinylated peptides were incubated with europium-labelled streptavidin and fluorescence was detected. ursula.holzer@med.uni‐tuebingen.de Accepted 2017-MAY-03 AT 09:24 BST AT 09:24 BST by ORCHARD European journal of immunology 2007 imex curation I have altered all peptides to "Chem. synthesis" and altered Interaction Type from Direct to Physical when there are 3 participants - I assume this is what you meant. However there are 5 instances of variants/mutations so I dont see why you have just selected one to alter? The definition of a "Variant" is "A natural change in a sequence or structure in comparison to a reference entity." so I dont see what difference it makes if they are entered as "mutations" or "variants" or if they are synthetic or not- as I have entered all other instances (full length?) of these as "variants" and this is what they are usually referred to. drb1_human HLA class II histocompatibility antigen, DRB1 beta chain HLA-DRB1 Human leukocyte antigen DRB1 protein protein human Homo sapiens Human MVCLKLPGGSCMTALTVTLMVLSSPLALSGDTRPRFLWQPKRECHFFNGTERVRFLDRYFYNQEESVRFDSDVGEFRAVTELGRPDAEYWNSQKDILEQARAAVDTYCRHNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFLNGQEEKAGMVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS 3B5912820A4654BE dnak_ecoli Chaperone protein DnaK dnaK groP grpF seg b0014 Heat shock protein 70 Heat shock 70 kDa protein HSP70 JW0013 protein protein ecoli Escherichia coli (strain K12) MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK DIP protein P0A6Y8 original sequence version: 44 DIP protein P0A6Y8 original sequence version: 96 DIP protein P0A6Y8 original sequence version: 91 DIP protein P0A6Y8 original sequence version: 119 DIP protein P0A6Y8 original sequence version: 95 5A8589B21D7CD9C1 d7rig0_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKLPGGSCMTALTVTLMVLSSPLALAGDTRPRFLWQLKFECHFFNGTERVRLLERCIYNQEESVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQRRAAVDTYCRHNYGVGESFTVQRRVEPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS CC9CC7E2D0DD036C hg2a_human HLA class II histocompatibility antigen gamma chain DHLAG HLA-DR antigens-associated invariant chain Ia antigen-associated invariant chain CD74 protein protein human Homo sapiens Human MHRRRSRSCREDQKPVMDDQRDLISNNEQLPMLGRRPGAPESKCSRGALYTGFSILVTLLLAGQATTAYFLYQQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKVLTKCQEEVSHIPAVHPGSFRPKCDENGNYLPLQCYGSIGYCWCVFPNGTEVPNTRSRGHHNCSESLELEDPSSGLGVTKQDLGPVPM 27A13F252D5FB91D a0a1v1igj9_human HLA-DRB1 protein protein human Homo sapiens Human MVCLKFPGGSCMAALTVTLMVLSSPLALAGDTRPRFLEQVKHECHFFNGTERVRFLDRYFYHQEEYVRFDSDVGEYRAVTELGRPDAEYWNSQKDLLEQKRAAVDTYCRHNYGVGESFTVQRRVYPEVTVYPAKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKTGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSLTSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS 8116E91DA38294E5 hspa1a_hspa1b_human-1 HSPA1A_HSPA1B_human molecule set molecule set human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD P0DMV8,P0DMV9 q0r326_human HLA-DRB1 HLA-DRB5 hCG_1998957 protein protein human Homo sapiens Human MVCLKLPGGSCMAALTVTLMVLSSPLALAGDTQPRFLKQDKFECHFFNGTERVRYLHRGIYNQEENVRFDSDVGEYRAVTELGRPVAESWNSQKDFLERRRAEVDTVCRHNYGVGESFTVQRRVHPEVTVYPAKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVMSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS AD46B4AD27BAD8F2 tetx_clote Tetanus toxin tetX Tentoxylysin CTC_p60 protein protein Clostridium tetani (strain Massachusetts / E88) Clostridium tetani (strain Massachusetts / E88) MPITINNFRYSDPVNNDTIIMMEPPYCKGLDIYYKAFKITDRIWIVPERYEFGTKPEDFNPPSSLIEGASEYYDPNYLRTDSDKDRFLQTMVKLFNRIKNNVAGEALLDKIINAIPYLGNSYSLLDKFDTNSNSVSFNLLEQDPSGATTKSAMLTNLIIFGPGPVLNKNEVRGIVLRVDNKNYFPCRDGFGSIMQMAFCPEYVPTFDNVIENITSLTIGKSKYFQDPALLLMHELIHVLHGLYGMQVSSHEIIPSKQEIYMQHTYPISAEELFTFGGQDANLISIDIKNDLYEKTLNDYKAIANKLSQVTSCNDPNIDIDSYKQIYQQKYQFDKDSNGQYIVNEDKFQILYNSIMYGFTEIELGKKFNIKTRLSYFSMNHDPVKIPNLLDDTIYNDTEGFNIESKDLKSEYKGQNMRVNTNAFRNVDGSGLVSKLIGLCKKIIPPTNIRENLYNRTASLTDLGGELCIKIKNEDLTFIAEKNSFSEEPFQDEIVSYNTKNKPLNFNYSLDKIIVDYNLQSKITLPNDRTTPVTKGIPYAPEYKSNAASTIEIHNIDDNTIYQYLYAQKSPTTLQRITMTNSVDDALINSTKIYSYFPSVISKVNQGAQGILFLQWVRDIIDDFTNESSQKTTIDKISDVSTIVPYIGPALNIVKQGYEGNFIGALETTGVVLLLEYIPEITLPVIAALSIAESSTQKEKIIKTIDNFLEKRYEKWIEVYKLVKAKWLGTVNTQFQKRSYQMYRSLEYQVDAIKKIIDYEYKIYSGPDKEQIADEINNLKNKLEEKANKAMININIFMRESSRSFLVNQMINEAKKQLLEFDTQSKNILMQYIKANSKFIGITELKKLESKINKVFSTPIPFSYSKNLDCWVDNEEDIDVILKKSTILNLDINNDIISDISGFNSSVITYPDAQLVPGINGKAIHLVNNESSEVIVHKAMDIEYNDMFNNFTVSFWLRVPKVSASHLEQYGTNEYSIISSMKKHSLSIGSGWSVSLKGNNLIWTLKDSAGEVRQITFRDLPDKFNAYLANKWVFITITNDRLSSANLYINGVLMGSAEITGLGAIREDNNITLKLDRCNNNNQYVSIDKFRIFCKALNPKEIEKLYTSYLSITFLRDFWGNPLRYDTEYYLIPVASSSKDVQLKNITDYMYLTNAPSYTNGKLNIYYRRLYNGLKFIIKRYTPNNEIDSFVKSGDFIKLYVSYNNNEHIVGYPKDGNAFNNLDRILRVGYNAPGIPLYKKMEAVKLRDLKTYSVQLKLYDDKNASLGLVGTHNGQIGNDPNRDILIASNWYFNHLKDKILGCDWYFVPTDEGWTND 18838FB2654024CF a0a0g4pyz0_human HLA-DRB1 protein protein human Homo sapiens Human MVCLRLPGGSCMAVLTVTLMVLSSPLALAGDTRPRFLEYSTGECYFFNGTERVRFLDRYFYNQEEYVRFDSDVGEYRAVTELGRPSAEYWNSQKDFLEDRRALVDTYCRHNYGVGESFTVQRRVHPKVTVYPSKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKTGVVSTGLIHNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVTSPLTVEWSARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS D452D1C3A75CEA31 hla_drb1-dnak-1 1 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1A, 3 HLA-DRB1*0801 allele The DRB1*0402 peptide did not inhibit binding, but all other peptides tested did. low pH hla_drb1-dnak-2 1 8 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 1B-1 HLA-DRB1*0801 allele Inhibitor:HLA-DRB1*0901/DRB1*090102 allele (0901 sequence shown to contain errors and be identical to DRB1*09:01:02 (December 1996)). hla_drb1-dnak-3 1 6 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.[Lys100Arg;Ala103Glu] mutation mutation certain certain sequence position certain certain certain sequence position certain A E certain certain sequence position certain certain certain sequence position certain K R Wed May 02 15:33:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys100arg,ala103glu' chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 1B-2 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0403 hla_drb1-dnak-4 1 6 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 1B-3 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0401 hla_drb1-dnak-5 1 4 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 1B-4 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0101 hla_drb1-dnak-6 1 10 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 1B-5 HLA-DRB1*0801 allele Inhibitor: HLA-DRB1*0801 dnak-hla_drb1-1 1 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2A The DRB1*0402 peptide did not inhibit binding, but all other peptides tested did. dnak-hla_drb1-2 1 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 inhibitor inhibitor prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-7 Inhibitor:HLA-DRB1*0801 allele dnak-hla_drb1-3 1 6 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-1 Inhibitor: HLA-DRB1*0401 dnak-hla_drb1-4 1 4 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-4 Inhibitor: HLA-DRB1*0101 dnak-hla_drb1-5 1 8 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-5 Inhibitor:HLA-DRB1*0901/DRB1*090102 allele (0901 sequence shown to contain errors and be identical to DRB1*09:01:02 (December 1996)). dnak-hla_drb1-6 1 6 inhibitor inhibitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.[Lys100Arg;Ala103Glu] mutation mutation certain certain sequence position certain certain certain sequence position certain A E certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:32:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys100arg,ala103glu' chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-6 Inhibitor: HLA-DRB1*0403 dnak-cd74-1 1 5 inhibitor inhibitor prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-8 dnak-cd74-2 1 9 inhibitor inhibitor prey prey fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2A-9 hspa1a-cd74-1 1 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction 2B, 7A Unlabeled HLA-DR-derived peptide fragments showed very weak competition I have not added individual HLA-DR peptides - hope this is OK? ATP hspa1a-cd74-2 1 5 inhibitor inhibitor prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 2Bi Unlabeled CLIP peptide as well as a peptide from tetanus toxin (TT 947–966) showed stronger competition as compared to the HLA-DR-derived peptide fragments. hspa1a-cd74-3 1 9 inhibitor inhibitor prey prey fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey clip_fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 2Bii Unlabeled CLIP peptide as well as a peptide from tetanus toxin (TT 947–966) showed stronger competition as compared to the HLA-DR-derived peptide fragments. dnak-hla_drb1-7 1 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 A/B, 5, 6,7B HLA-DRB1*0401 Binding is greater at pH 5 than pH 7. Hsc70 also bound HLA-DRB1*0401 to a greater extent at pH=5 than pH 7, but the origins of this molecule could not be clarified. pH7 - Kd =113nM (as shown in figure 5) pH5 - Kd = 15nM (as shown in figure 5) From several experiments, a KD of 130 nM was calculated at pH 7 and of 26.6 nM at pH 5. (results not shown) ATP dnak-hla_drb1-8 1 2 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 3 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 A/Bi HLA-DRB1*0401 weak binding hspa1a_hspa1b_human-hla_drb1-1 1 2 inhibitor inhibitor prey prey human-ihw 9041/jo 528239 human-ihw 9041/jo 528239 B Lymphoblastoid Cell Line Human ihw 9041/jo 528239 IHW 9041/JO 528239 http://www.ebi.ac.uk/cgi-bin/ipd/imgt/hla/fetch_cell.cgi?11636 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 6-1 HLA-DRB1*0401;Inhibitor HLA-DRB1*1104 hspa1a_hspa1b_human-hla_drb1-2 1 2 inhibitor inhibitor prey prey human-bm9 human-BM9_B Lymphoblastoid Cell Line Human bm9 bm9-IHW 9068 EBV transformed B lymphoblastoid cell line http://www.ebi.ac.uk/cgi-bin/ipd/imgt/hla/fetch_cell.cgi?11619 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 6-2 HLA-DRB1*0401;Inhibitor HLA-DRB1*0801 hspa1a_hspa1b_human-hla_drb1-3 1 2 inhibitor inhibitor prey prey human-pitout human-Pitout IHW9051 Human pitout PITOUT-IHW 9051 B Lymphoblastoid Cell Line http://web.expasy.org/cellosaurus/CVCL_E809 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 6-3 HLA-DRB1*0401;Inhibitor HLA-DRB1*0701 hspa1a_hspa1b_human-hla_drb1-4 1 2 inhibitor inhibitor prey prey human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 6-4 HLA-DRB1*0401;Inhibitor HLA-DRB1*0401 Using recombinant HLA-DRB1*0401 lacking the transmembrane domain produced the same result. hspa1a_hspa1b_human-hla_drb1-5 1 6 inhibitor inhibitor prey prey p.[Leu96Ile;Gln99Asp;Lys100Glu;Gly115Val] mutation mutation certain certain sequence position certain certain certain sequence position certain Q D certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain G V certain certain sequence position certain certain certain sequence position certain L I Wed May 02 20:17:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu96ile,gln99asp,lys100glu,gly115val' human-al10 AL10- EBV-transformed B lymhoblastoid cell line from RA patient Human al10 AL10- EBV-transformed B lymhoblastoid cell line from RA patient 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 6-5 HLA-DRB1*0401;Inhibitor HLA-DRB1*0402 Using recombinant HLA-DRB1*0402 lacking the transmembrane domain produced the same result. hspa1a_hspa1b_human-hla_drb1-6 1 2 inhibitor inhibitor prey prey P01911:p.Val115Gly mutation mutation certain certain sequence position certain certain certain sequence position certain V G Wed May 02 15:12:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val115gly' Tue Jun 30 17:51:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val115Gly' human-hor human HOR/IHW 9053 B Lymphoblastoid Cell Line Human hor-ihw 9053 HOR-IHW 9053 http://www.ebi.ac.uk/cgi-bin/ipd/imgt/hla/fetch_cell.cgi?10757 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 6-6 HLA-DRB1*0401;Inhibitor HLA-DRB1*1302 hspa1a_hspa1b_human-hla_drb1-7 1 6 inhibitor inhibitor prey prey HV3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 2 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-savc human SAVC-EBV transformed lymphoblastoid line Human savc SAVC-EBV transformed lymphoblastoid line ECACC No 95042723 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) physical association physical association 7C HLA-DRB1*0401;Inhibitor HLA-DRB1*0401 The interaction of Hsp70 with complete HLA-DR molecules cannot be significantly inhibited by HV3-region peptide fragments.