DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Nature structural & molecular biology 2007 imex curation Buker SM., Iida T., Bühler M., Villén J., Gygi SP., Nakayama J., Moazed D. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Buker SM., Iida T., Bühler M., Villén J., Gygi SP., Nakayama J., Moazed D. DIP original experiment: DIP-66676X Nature structural & molecular biology 2007 IMEx Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Nature structural & molecular biology 2007 imex curation Buker SM., Iida T., Bühler M., Villén J., Gygi SP., Nakayama J., Moazed D. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Buker SM., Iida T., Bühler M., Villén J., Gygi SP., Nakayama J., Moazed D. DIP original experiment: DIP-66674X Nature structural & molecular biology 2007 IMEx Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. Nature structural & molecular biology 2007 imex curation Buker SM., Iida T., Bühler M., Villén J., Gygi SP., Nakayama J., Moazed D. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip fingerprinting peptide massfingerprinting fingerprinting Buker SM., Iida T., Bühler M., Villén J., Gygi SP., Nakayama J., Moazed D. DIP original experiment: DIP-66675X Nature structural & molecular biology 2007 IMEx Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast. ago1_schpo Protein argonaute Eukaryotic translation initiation factor 2C 2-like protein ago1 PAZ Piwi domain protein ago1 Cell cycle control protein ago1 RNA interference pathway protein ago1 ago1 csp9 SPCC736.11 Protein slicer protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSYKPSSEIALRPGYGGLGKQITLKANFFQIISLPNETINQYHVIVGDGSRVPRKQSQLIWNSKEVKQYFGSSWMNSVYDGRSMCWSKGDIADGTIKVNIGSESHPREIEFSIQKSSKINLHTLSQFVNSKYSSDPQVLSSIMFLDLLLKKKPSETLFGFMHSFFTGENGVSLGGGVEAWKGFYQSIRPNQGFMSVNVDISSSAFWRNDSLLQILMEYTDCSNVRDLTRFDLKRLSRKFRFLKVTCQHRNNVGTDLANRVYSIEGFSSKSASDSFFVRRLNGEEQKISVAEYFLENHNVRLQYPNLPCILVKNGAMLPIEFCFVVKGQRYTAKLNSDQTANMIRFAVQRPFERVQQIDDFVHQMDWDTDPYLTQYGMKIQKKMLEVPARVLETPSIRYGGDCIERPVSGRWNLRGKRFLDPPRAPIRSWAVMCFTSTRRLPMRGIENFLQTYVQTLTSLGINFVMKKPPVLYADIRGSVEELCITLYKKAEQVGNAPPDYLFFILDKNSPEPYGSIKRVCNTMLGVPSQCAISKHILQSKPQYCANLGMKINVKVGGINCSLIPKSNPLGNVPTLILGGDVYHPGVGATGVSIASIVASVDLNGCKYTAVSRSQPRHQEVIEGMKDIVVYLLQGFRAMTKQQPQRIIYFRDGTSEGQFLSVINDELSQIKEACHSLSPKYNPKILVCTTQKRHHARFFIKNKSDGDRNGNPLPGTIIEKHVTHPYQYDFYLISHPSLQGVSVPVHYTVLHDEIQMPPDQFQTLCYNLCYVYARATSAVSLVPPVYYAHLVSNLARYQDVTADDTFVETSEASMDQEVKPLLALSSKLKTKMWYM F5CE397BF590D9E4 F5CE397BF590D9E4 arb2_schpo Argonaute-binding protein 2 arb2 SPAC13G7.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFRKKKSRVLQRDVNFGNCLEDMNLQITNDFYLRKLNAPNELYEFQVTKDKEYNEKFYFAVLDHVYSWTLQNSLLTRISIPLESSPSEPHSWIYATRNWQTNENGIVIIIQSMSHPLIWSNRNLREGDVRRATVLDVIAKCTEKKLSVVIFSPSALLWDQSINFPRTTRYFTSQGLQITKKSIIKDVETPESYVSIGLNYVMSNSVSTKIHFVGSEYGSYLLNSYLDLNWKKFSGKAGAILLIMNMSINEELHDPDYVSYLIEHARNYLPSHEPCRHLMNSIMTESGIPNFSAGKYIDEFYDYIGDVLLEMIALDTNTSKS C675802B4B945297 arb1_schpo Argonaute-binding protein 1 arb1 SPAC140.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAEGDFKNDSTGCIGDSVEFTTFKKRTGKSLGNRRKKRGLSGWEPYFQEPELTAEEKLENETLYSRNIPLTVRMERFIQRYRSRRKWSDPTRIRLFTMYLDLGGVSTGQKQFTGGTDINNDAKAREVSAQTTTDYIEYDILDEYDIDFKWVAGVFLSSHILYNAGLTKEEDLQIACQIVRNFLLSVLHNNVAPEFEDNIRDACLLAEQAETELISNKRLSTKLPGRLQRALASIHVDNYKGLWDKSQSDRTSDSSVNFIESNNTKFMPNDELFEPDGISRFSTQQARDYIQRTLGPRYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSKYSQDTNKERLEVILESDLLNNTVDGTHLEGSFTFLDNGLTILDTVLAVLPTFYEEVKDE 6508689C97C5F1D5 hrr1_schpo Helicase required for RNAi-mediated heterochromatin assembly 1 hrr1 SPCC1739.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEQVQDEIWKLSTLDAWEMVNKNTEVVFDEIPEPETLSEMKRHPLYSNIFNADNNTTSFTEQIETSETSKTQDSEGNKVDKNLKENKSIRRKRSIDNDYELSNVKRNDITSGKNREFENEHHPASDTSSWRELPSIPTLEELTSKSVELPSNNIYGGYKSFEDYLSIHYRLLREDAVSPLRESVLRYKVNPNYITGSSLAVYDHVRIDGYTISSSVIAAKLSFSVRAKKKIKWATSRRLISGSLVLLSNDDFQTFRIGTVCARPLSGLNKHPHEIDVKFEDISISLDPREEYVMIEATSGYWEAYKHVLRSLQRLSASTFPMKDYLVHCKSNQETAKHIQNNPRIRINSILKNNSQKIVNALEPFGPGEYILDSSQLKAYQSMLTKRLSIIQGPPGTGKSFVTLKAIETLLENTHSHVLPILVACQTNHAVDQILIRLLHQGASVMRLGSRTKDPEIAAVTIFQKAKHTKHSFKAAYNEIRHKKQRLIKQITNIMHNFNLEFVTLSYLHSKGIITTSQLESLRNNTEWISSVAENGEKTEEELISIWLGDAKVELITPSEITDGFEEELQIDPEKLEEIQKEAEDSGALMEEELRGKFINLRCKYLFSKLTTLHEKEIDTLLTIPNIWDIPEYSRGIIYCRWLESAYAAAEKELNRLYRFYLKVDRERIGFSNKRAAILLRGANVIGMTTTGLNKYRDILERINPKICFIEEAADVLEGPIIPAVFPSLEQLVLIGDHKQLRPGCSTYALRQDPFNLSISMFERLVENDMEYTRLTMQRRMHPQIRRLVSSVYEDLSDYEITKYWPSIPGMGEIRRFFLTHSRIEDNDGFASKINLFEAQMLVQFAVYLINNGVEPQKITCLTFYAAQKDLIERLLSESLNREKHFIKVATVDGYQGEENDVVLLSLVRNNDRTEVGFLSSPHRVCVSLSRARRGLFIFGNAQLVAESNPLWWDAINTLMNDETIQGLGDHLPLFTKDGTIYVNDPVELLDVNMRLTRK 8CBE21AF974D56CC tas3_schpo RNA-induced transcriptional silencing complex protein tas3 SPBC83.03c tas3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEKGIKKYLPSLPFLACISDFPENHGTSRRSATVSLERVHELFTEHWLSNLKNRREKRQELAEEAVYCRSEMLSQRKLLAAVDFPQQLKNSPAKAKATHTSSGVTKEVRASKKYTSSNVEFPLVTDGKEKPVKSKQLRKNSVTEFEKPIETKKSKHRKSRNKFLDKSSGSMEIESWDNSTSDSIIESSSRLHESISLRENDIRSSDSKSVGWDDNSTGFRESSKSLDHTDTSMFMELDSNSDPQFRPKYQAKSSWFAPDDPEASWGNLDDGWGETNGSWSSTDDTKHYKNEWAESINLDNFNRPSQQEDYDKPKNTQVSRSSNHHRRYDSYHPDSRSDSYRSKREHYDNRDTGPRSKHLEKSSYVYNQNFEDRTHLSDHGAHFHLGNANDFNMQGSSRKRKASDRQRESRENELPTKKLNASDSHNPLASLTTDKNDLYINWLKSLSFFQTNSSCAEALVKVIPHYHNKLIDFSQVLQLVFSASEKFPIQENQPLPEQLMFLSNLEKQTPFAKAVGSSIYKLVTGKNLSLDFASQILKEASILEHKNEK 4569CF59851A22A0 4569CF59851A22A0 chp1_schpo Chromo domain-containing protein 1 chp1 SPAC18G6.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVSVKPLPDIDSNEGETDADVYEVEDILADRVNKNGINEYYIKWAGYDWYDNTWEPEQNLFGAEKVLKKWKKRKKLIAKGLLEPFDAEDNEAKKMKREKEILRQQRQKRKSELTQLSQKVKEKFKKMRKKPARRIVTIANDEEEEDDQTMDEDAFERKSMQGELKERNLTDKTSTLSTSFGETSPDVNPFYLSEWPTVTDSILLSKSLSSDAIPLKNGEIKSTMLMPSDSDNSVPGIQNSNNLENTGAFVENANSPQSNTPLSTFRHSSPLSLSPVITSDNDVANSLFFSNSTPLPSSLKIKKEAPKLETHTILVSDNSGSLTKQDILSYFAFIKGNIEVFFLKSPKKDKVCNMAYIQFDSIEQAKLAYDKGHPSWHVTLVKGKISTDMEECKVSKSILKTTPSKKANARSVSFTQTTTDTLSESEKFASNVDLDENFDFNVNVTNEDAKQLKKSVIGSSWTTVNNDWNSVSKSDQTFENDGASKVVPAGNITLNSDNSLHHSISESEDLSSASTLSDYFRFVLRVGKSLYYAGELSFDISKLKAETEHQQLLRSLVSCKQVDVLRFVTSQYLEVFGTCLTKVLSGSLCIRSDVDMTHFKNILNRGNGAGIVLGSNYTLLLFTEDNNALMNLYDCQGQSNSPFWMVIFEPLESILVEWSAKNLRPKKPYHKSQSYLSYLLQLGHIDLHKIGAFQATQILIVSKQPSPEAEELEDTFREAAIPTFRGLEIPESLFLSQNVFVFLNVSLEDDFDQLQFLTLAKRKSCKFFLFGLSLPLKSPNDSHVGTDFKKNNEPLDKLTYSQYLRPMFPKGGVVSVTLSALIKTPRLLELISPFLEIKKDSWILILPPSIVDMVKSYFVTNNPDKSLLEIQNLLNTLQRYLTNPALKNVTLYQDWDIVIDDSADVSLASTLQLYQKKNYDKYRRFVLIHELKNELTPVNGLDIVDYDEFKETFMRAIGLK E92C16BBD0D5957C E92C16BBD0D5957C ago1-arb1-1 2 7 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 8 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 9 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 5 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 6 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 4 unspecified role unspecified role bait bait Flag Tag Flag Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast association association ago1-arb2-1 3 5 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 4 unspecified role unspecified role bait bait Myc Tag Myc Tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association arb1-ago1-1 1 5 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 6 unspecified role unspecified role bait bait TAP Tag TAP Tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 4 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast association association