DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. Nature 2007 imex curation Li W., Tu D., Brunger AT., Ye Y. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li W., Tu D., Brunger AT., Ye Y. DIP original experiment: DIP-255312X Nature 2007 IMEx A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. Nature 2007 imex curation Li W., Tu D., Brunger AT., Ye Y. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Li W., Tu D., Brunger AT., Ye Y. DIP original experiment: DIP-255315X Nature 2007 IMEx A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. Nature 2007 imex curation Li W., Tu D., Brunger AT., Ye Y. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Li W., Tu D., Brunger AT., Ye Y. DIP original experiment: DIP-255314X Nature 2007 IMEx A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. Nature 2007 imex curation Li W., Tu D., Brunger AT., Ye Y. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Li W., Tu D., Brunger AT., Ye Y. DIP original experiment: DIP-255316X Nature 2007 IMEx A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. Nature 2007 imex curation Li W., Tu D., Brunger AT., Ye Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Li W., Tu D., Brunger AT., Ye Y. DIP original experiment: DIP-255313X Nature 2007 IMEx A ubiquitin ligase transfers preformed polyubiquitin chains from a conjugating enzyme to a substrate. herp1_human Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein Methyl methanesulfonate (MMF)-inducible fragment protein 1 HERPUD1 KIAA0025 MIF1 HERP protein protein human Homo sapiens Human MESETEPEPVTLLVKSPNQRHRDLELSGDRGWSVGHLKAHLSRVYPERPRPEDQRLIYSGKLLLDHQCLRDLLPKQEKRHVLHLVCNVKSPSKMPEINAKVAESTEEPAGSNRGQYPEDSSSDGLRQREVLRNLSSPGWENISRPEAAQQAFQGLGPGFSGYTPYGWLQLSWFQQIYARQYYMQYLAATAASGAFVPPPSAQEIPVVSAPAPAPIHNQFPAENQPANQNAAPQVVVNPGANQNLRMNAQGGPIVEEDDEINRDWLDWTYSAATFSVFLSILYFYSSLSRFLMVMGATVVMYLHHVGWFPFRPRPVQNFPNDGPPPDVVNQDPNNNLQEGTDPETEDPNHLPPDRDVLDGEQTSPSFMSTAWLVFKTFFASLLPEGPPAIAN DIP protein Q15011 original sequence version: 131 DIP protein Q15011 original sequence version: 101 3CA827DC7EF0ED22 ub2g2_mouse Ubiquitin-conjugating enzyme E2 G2 Ube2g2 Ubiquitin carrier protein G2 Ubiquitin-protein ligase G2 Ubc7 E2 ubiquitin-conjugating enzyme G2 protein protein mouse Mus musculus Mouse MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL DIP protein P60605 original sequence version: 93 DIP protein P60605 original sequence version: 87 DIP protein P60605 original sequence version: 66 DIP protein P60605 original sequence version: 27 74DEC732A79575E3 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS DIP protein Q9UKV5 original sequence version: 136 DIP protein Q9UKV5 original sequence version: 122 DIP protein Q9UKV5 original sequence version: 113 DIP protein Q9UKV5 original sequence version: 85 DIP protein Q9UKV5 original sequence version: 103 DIP protein Q9UKV5 original sequence version: 40 8782324609C0E62A amfr-amfr-4 1 9 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 9 unspecified role unspecified role bait bait Region 309-643 Region 309-643 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association ub-ube2g2-1 2 8 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli covalent binding covalent binding ube2g2-6 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction herpud1-ub-1 3 6 unspecified role unspecified role bait bait Region 1-259 Region 1-259 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q15011:p.Lys61Arg K61R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K61R'. Wed May 02 19:43:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys61arg' Tue Jun 30 19:26:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys61Arg' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli physical association physical association ub-ube2g2-2 4 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction