DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. DIP original experiment: DIP-261074X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. DIP original experiment: DIP-261073X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. DIP original experiment: DIP-261075X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. DIP original experiment: DIP-261072X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kastning K., Kukhtina V., Kittler JT., Chen G., Pechstein A., Enders S., Lee SH., Sheng M., Yan Z., Haucke V. DIP original experiment: DIP-261076X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Molecular determinants for the interaction between AMPA receptors and the clathrin adaptor complex AP-2. gria1_rat Glutamate receptor 1 Gria1 GluR-A GluR-K1 Glutamate receptor ionotropic, AMPA 1 AMPA-selective glutamate receptor 1 Glur1 protein protein rat Rattus norvegicus (Rat) Rat MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDSRDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGGGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL DIP protein P19490 original sequence version: 146 DIP protein P19490 original sequence version: 142 DIP protein P19490 original sequence version: 117 DIP protein P19490 original sequence version: 127 DIP protein P19490 original sequence version: 131 DIP protein P19490 original sequence version: 129 2D4CFA7CCD532838 gria2_rat Glutamate receptor 2 Gria2 Glur2 GluR-B GluR-K2 Glutamate receptor ionotropic, AMPA 2 AMPA-selective glutamate receptor 2 protein protein rat Rattus norvegicus (Rat) Rat MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI DIP protein P19491 original sequence version: 164 DIP protein P19491 original sequence version: 160 DIP protein P19491 original sequence version: 131 DIP protein P19491 original sequence version: 136 DIP protein P19491 original sequence version: 141 DEFA817027C1CCD1 gria3_rat Glutamate receptor 3 Gria3 Glur3 GluR-K3 Glutamate receptor ionotropic, AMPA 3 AMPA-selective glutamate receptor 3 GluR-C protein protein rat Rattus norvegicus (Rat) Rat MGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFHLNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALKGAILSLLSYYKWEKFVYLYDTERGFSVLQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMVQQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSSSSENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSALGNAVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEFCYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI DIP protein P19492 original sequence version: 141 2981616375661703 pep2r GluR2-derived Ap-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) CKRMKVAKNPQ ap2m1_rat AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain Ap2m1 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein rat Rattus norvegicus (Rat) Rat MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC DIP protein P84092 original sequence version: 91 DIP protein P84092 original sequence version: 42 DIP protein P84092 original sequence version: 82 DIP protein P84092 original sequence version: 67 DIP protein P84092 original sequence version: 92 DIP protein P84092 original sequence version: 105 DIP protein P84092 original sequence version: 110 82803219BA279954 gria2-ap2m1-1 4 10 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P19491:p.Lys844Ala K844A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K844A'. Wed May 02 16:43:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys844ala' Tue Jun 30 17:52:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys844Ala' ecolx Escherichia coli direct interaction direct interaction gria1-ap2m1-1 1 6 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 157-435 Region 157-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range rat Rattus norvegicus (Rat) Rat direct interaction direct interaction gria2-ap2m1-2 2 10 unspecified role unspecified role prey prey Region 157-435 Region 157-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range rat Rattus norvegicus (Rat) Rat 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P19491:p.Lys844Ala K844A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K844A'. Wed May 02 18:11:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys844ala' Tue Jun 30 19:43:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys844Ala' P19491:p.Arg845Ala R845A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R845A'. Wed May 02 19:30:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg845ala' Tue Jun 30 18:53:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg845Ala' P19491:p.Lys847Ala K847A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K847A'. Wed May 02 17:30:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys847ala' Tue Jun 30 15:52:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys847Ala' ecolx Escherichia coli direct interaction direct interaction ap2m1-pep2r-1 5 9 unspecified role unspecified role prey prey in vitro In vitro 10 unspecified role unspecified role bait bait Region 157-435 Region 157-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rat Rattus norvegicus (Rat) Rat direct interaction direct interaction gria3-ap2m1-1 3 8 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 157-435 Region 157-435 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range rat Rattus norvegicus (Rat) Rat direct interaction direct interaction