DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T., Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-264214X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T., Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-264213X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T., Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Sadowski MJ., Pankiewicz J., Scholtzova H., Mehta PD., Prelli F., Quartermain D., Wisniewski T. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-264212X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Blocking the apolipoprotein E/amyloid-beta interaction as a potential therapeutic approach for Alzheimer's disease. apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH DIP protein P02649 original sequence version: 197 DIP protein P02649 original sequence version: 146 91AFC04210A30689 p05067-pro_0000000093 Amyloid-beta protein 40 APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 672 711 D563D7858C3768F1 app-apoe-1 3 4 unspecified role unspecified role prey prey mouse Mus musculus Mouse 5 unspecified role unspecified role bait bait Region 12-28 Region 12-28 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 1 apoe-app-2 2 4 unspecified role unspecified role unspecified role unspecified role mouse Mus musculus Mouse 5 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction app-app-75 1 5 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction