DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Nature 2006 imex curation Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. Only protein-protein interactions in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. DIP original experiment: DIP-254838X Nature 2006 IMEx Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Nature 2006 imex curation Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. Only protein-protein interactions in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. DIP original experiment: DIP-254837X Nature 2006 IMEx Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Nature 2006 imex curation Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. Only protein-protein interactions in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. DIP original experiment: DIP-254835X Nature 2006 IMEx Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Nature 2006 imex curation Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. DIP original experiment: DIP-254839X Nature 2006 IMEx Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Repression of p53 activity by Smyd2-mediated methylation. Nature 2006 imex curation Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. Only protein-protein interactions in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Huang J., Perez-Burgos L., Placek BJ., Sengupta R., Richter M., Dorsey JA., Kubicek S., Opravil S., Jenuwein T., Berger SL. DIP original experiment: DIP-254836X Nature 2006 IMEx Repression of p53 activity by Smyd2-mediated methylation. setd7_human Histone-lysine N-methyltransferase SETD7 SETD7 KIAA1717 SET7 KMT7 SET9 Histone H3-K4 methyltransferase SETD7 SET domain-containing protein 7 SET7/9 Lysine N-methyltransferase 7 protein protein human Homo sapiens Human MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK DIP protein Q8WTS6 original sequence version: 46 DIP protein Q8WTS6 original sequence version: 89 DIP protein Q8WTS6 original sequence version: 106 DIP protein Q8WTS6 original sequence version: 120 DIP protein Q8WTS6 original sequence version: 121 DIP protein Q8WTS6 original sequence version: 114 DIP protein Q8WTS6 original sequence version: 95 73A1217079E3BA13 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 smyd2_mouse N-lysine methyltransferase SMYD2 Smyd2 Histone methyltransferase SMYD2 SET and MYND domain-containing protein 2 protein protein mouse Mus musculus Mouse MRAEARGGLERFCSAGKGRGLRALRPFHVGDLLFSCPAYACVLTVGERGHHCECCFARKEGLSKCGRCKQAFYCDVECQKEDWPLHKLECSSMVVLGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVREFESHLDKLDNEKKDLIQSDIAALHQFYSKYLEFPDHSSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIHPGDEVFTSYIDLLYPTEDRNDRLRDSYFFTCECRECTTKDKDKAKVEVRKLSSPPQAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVCLYMQDWEGALKYGQKIIKPYSKHYPVYSLNVASMWLKLGRLYMGLENKAAGEKALKKAIAIMEVAHGKDHPYISEIKQEIESH DIP protein Q8R5A0 original sequence version: 75 smyd2-tp53-2 2 8 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 361-380 Region 361-380 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04637:p.Lys370Arg K370R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K370R'. Wed May 02 17:23:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys370arg' Tue Jun 30 16:48:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys370Arg' K370me2 K370me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm K370me1 K370me1 me1lys monomethylated L-lysine Me1Lys Methyl Methylation methylk certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm K370me3 K370me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm in vitro In vitro methylation methylation reaction methylation setd7-tp53-2 3 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 361-380 Region 361-380 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04637:p.Lys372Arg K372R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K372R'. Wed May 02 18:33:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys372arg' Tue Jun 30 18:35:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys372Arg' in vitro In vitro methylation methylation reaction methylation tp53-smyd2-1 4 8 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association setd7-tp53-3 5 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 361-381 Region 361-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K372me2 K372me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm in vitro In vitro methylation methylation reaction methylation smyd2-tp53-3 1 8 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 361-381 Region 361-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K372me2 K372me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm K372me1 K372me1 me1lys monomethylated L-lysine Me1Lys Methyl Methylation methylk certain certain sequence position certain certain certain sequence position certain decreasing-ptm ptm decreasing an interaction decreasing-ptm in vitro In vitro methylation methylation reaction methylation