DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Nature structural & molecular biology 2006 imex curation Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66573X Nature structural & molecular biology 2006 IMEx Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. Nature structural & molecular biology 2006 imex curation Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66574X Nature structural & molecular biology 2006 IMEx Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain. ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 vps36_mouse Vacuolar protein-sorting-associated protein 36 Vps36 ESCRT-II complex subunit VPS36 protein protein mouse Mus musculus Mouse MDRFVWTSGLLEINETLVIQQRGVRVYDGEEKIKFDAGTLLLSTHRLIWRDQKNNECCMAIPLSQIVFIEEQAAGIGKSAKIVVHLHPAPSNKEPGPFQSSKNSYIRLSFKEHGQIEFYRRLSEEMTQRRWETVPVSQSLQTNKGPQPGRVRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKEKQGDVTEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQAPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPIRLRVFDSGVMVIELQTHKEEEMVASALETVSERGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQN DIP protein Q91XD6 original sequence version: 36 mint Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL 0036479499DD89C8 ub-vps36-3 1 4 unspecified role unspecified role neutral component neutral component 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 3 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction vps36-ub-2 2 4 unspecified role unspecified role bait bait 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q91XD6:p.Val67Ala V67A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A mutation'. Wed May 02 15:46:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala' Tue Jun 30 17:24:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val67Ala' Q91XD6:p.Phe68Ala F68A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F68A mutation'. Wed May 02 19:19:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe68ala' Tue Jun 30 15:54:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe68Ala' Q91XD6:p.Glu70Ala E70A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E70A mutation'. Wed May 02 17:02:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu70ala' Tue Jun 30 19:35:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu70Ala' Q91XD6:p.Val83Ala V83A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V83A mutation'. Wed May 02 20:27:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val83ala' Tue Jun 30 17:42:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val83Ala' Q91XD6:p.His85Ala H85A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H85A mutation'. Wed May 02 15:25:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his85ala' Tue Jun 30 19:01:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His85Ala' GST Tag GST Tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 3 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction