DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. DIP original experiment: DIP-263947X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. DIP original experiment: DIP-263949X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. DIP original experiment: DIP-263946X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wahl-Schott C., Baumann L., Cuny H., Eckert C., Griessmeier K., Biel M. DIP original experiment: DIP-263948X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain. calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62161 original sequence version: 125 DIP protein P62161 original sequence version: 51 DIP protein P62161 original sequence version: 55 DIP protein P62161 original sequence version: 59 DIP protein P62161 original sequence version: 80 DIP protein P62161 original sequence version: 81 DIP protein P62161 original sequence version: 132 DIP protein P62161 original sequence version: 96 6B4BC3FCDE10727B cac1f_mouse Voltage-dependent L-type calcium channel subunit alpha-1F Voltage-gated calcium channel subunit alpha Cav1.4 Cacna1f protein protein mouse Mus musculus Mouse MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVAVASAQRSPRALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVVNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDPSPCASSGSGRSCTLNHTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTDTPGDEDEEEGTMASCTRCLNKIMKTRICRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPLWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGREKSSEGNPPKENKVLVPGGENEDAKGARSEGAAPGMEEEEEEEEEEEEEEEEEEENGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKESKGSFLIYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSRAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDTSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGLVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREAPTSTSSALQAGLRSLQDLGPEIRQALTYVTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQDNQNEEQELPDWTPDLDRAGRDSFEPSPFTTSLVQQHVNGHMSTPTFAAPHACRSEPSFTIQCLQRLGSCEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTISLYSDEESILSRFDEEDLGDEMACVHAL DIP protein Q9JIS7 original sequence version: 110 p15381-4 Voltage-dependent L-type calcium channel subunit alpha-1C CACNA1C CACH2 CACN2 CACNL1A1 CCHL1A1 Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle Smooth muscle calcium channel blocker receptor Voltage-gated calcium channel subunit alpha Cav1.2 Lung protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNRGTPAGLHAQKKGKFAWFSHSTETHVSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKVMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVGKPALEEAKEEKIELKSITADGESPPTTKINMDDLQPNESEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVHPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPHNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDSRSAFPQTFTTQRPLHISKAGNNQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRLPRPAGYPSTVSTVEGHGSPLSPAVRAQEAAWKLSSKRCHSQESQIAMACQEGASQDDNYDVRIGEDAECCSEPSLLSTEMLSYQDDENRQLAPPEEEKRDIRLSPKKGFLRSASLGRRASFHLECLKRQKNQGGDISQKTVLPLHLVHHQALAVAGLSPLLQRSHSPTSLPRPCATPPATPGSRGWPPQPIPTLRLEGADSSEKLNSSFPSIHCGSWSGENSPCRGDSSAARRARPVSLTVPSQAGAQGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDLTIEEMENAADDILSGGARQSPNGTLLPFVNRRDPGRDRAGQNEQDASGACAPGCGQSEEALADRRAGVSSL calm_bovin Calmodulin CALM CAM protein protein bovin Bos taurus (Bovine) Bovine MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62157 original sequence version: 125 DIP protein P62157 original sequence version: 58 DIP protein P62157 original sequence version: null 6B4BC3FCDE10727B cacna1f-calm1-1 4 5 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal Invalid range Feature range: Line: 320, Character: 10: It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0), It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0) human Homo sapiens Human 6 unspecified role unspecified role bait bait Region 1445-1984 Region 1445-1984 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cacna1f-calm-1 3 8 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 1445-1609 Region 1445-1609 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction cacna1c-calm-1 1 8 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 1502-1666 Region 1502-1666 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction cacna1c-calm1-12 2 5 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal Invalid range Feature range: Line: 1291, Character: 10: It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0), It is not consistent to have a 'n-terminal' range position with a value different from 1 because 'n-terminal' means the first amino acid of the participant. Actual position : (0-0) human Homo sapiens Human 7 unspecified role unspecified role bait bait Region 1502-2166 Region 1502-2166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association