DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264109X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264106X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264110X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264108X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264105X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264104X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hale BG., Jackson D., Chen YH., Lamb RA., Randall RE. DIP original experiment: DIP-264107X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Influenza A virus NS1 protein binds p85beta and activates phosphatidylinositol-3-kinase signaling. ns1_i34a1 Non-structural protein 1 NS NS1A protein protein i34a1 Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) MDPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILKEESDEALKMTMASVPASRYLTDMTLEEMSRDWSMLIPKQKVAGPLCIRMDQAIMDKNIILKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSLPGHTAEDVKNAVGVLIGGLEWNDNTVRVSETLQRFAWRSSNENGRPPLTPKQKREMAGTIRSEV DIP protein P03496 original sequence version: 47 DIP protein P03496 original sequence version: 108 DIP protein P03496 original sequence version: 92 2F7EC18E3EE7A4BE ns1_i33a0 Non-structural protein 1 NS NS1A protein protein i33a0 Influenza A virus (strain A/Wilson-Smith/1933 H1N1) Influenza A virus (strain A/WS/1933 H1N1) MDPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILKEESDEALKMTMASVPASRYLTDMTLEEMSRHWFMLMPKQKVAGPLCIRMDQAIMDKNIILKANFSVILDRLETLILLRAFTEEGTIVGEISPLPSLPGHTDEDVKNAVGVLIGGLEWNNNTVRVSETLQRFAWRSSNENGRPPLTPKQKRKMAGTIRSEV DIP protein Q82506 original sequence version: 71 DIP protein Q82506 original sequence version: 67 E33D5512E090CF08 ns1_i72a2 Non-structural protein 1 NS NS1A protein protein i72a2 Influenza A virus (strain A/Udorn/307/1972 H3N2) MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQKSLRGRGSTLGLNIEAATHVGKQIVEKILKEESDEALKMTMASTPASRYITDMTIEELSRDWFMLMPKQKVEGPLCIRIDQAIMDKNIMLKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSFPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWGSSNENGRPPLTPKQKRKMARTARSKVRRDKMAD DIP protein P03495 original sequence version: 109 DIP protein P03495 original sequence version: 73 DIP protein P03495 original sequence version: 88 DIP protein P03495 original sequence version: 101 14D0308795627E5E v_sendf Protein V P/V/C protein protein sendf Sendai virus (strain Fushimi) MDQDAFILKEDSEVEREAPGGRESLSDVIGFLDAVLSSEPTDIGGDRSWLHNTINTPQGPGSAHRAKSEGEGEVSTPSTQDNRSGEESRVSGRTSKPEAEAHAGNLDKQNIHRAFGGRTGTNSVSQDLGDGGDSGILENPPNERGYPRSGIEDENREMAAHPDKRGEDQAEGLPEEVRGGTSLPDEGEGGASNNGRSMEPGSSHSARVTGVLVIPSPELEEAVLRRNKRRPTNSGSKPLTPATVPGTRSPPLNRYNSTGSPPGKPPSTQDEHINSGDTPAVRVKDRKPPIGTRSVSDCPANGRPIHPGLETDSTKKGHRREHIIYERDGYIVDESWCNPVCSRIRVIPRRELCVCKTCPKVCKLCRDDIQCMRPDPFCREIFRS DIP protein P69284 original sequence version: 40 765F3110EDD9B30A p85b_bovin Phosphatidylinositol 3-kinase regulatory subunit beta Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta PIK3R2 protein protein bovin Bos taurus (Bovine) Bovine MAGPEGFQYRALYPFRRERPEDLELLPGDVLVVSRAALQALGVAEGNERCPQSVGWMPGLNERTRQRGDFPGTYVEFLGPVALARPGPRPRGPRPLPARPRDGPPEPGLTLPDLPEQFSPPDVAPPILVKLVEAIERTGLDSYRPEPPAVRTDWSLSDVEQWDAAALSDGVKGFLLALPAPLVTPEAAAEAHRALREAAGPVGPALEPPTLPLHHALTLRFLLQHLGRVAGRAPAPGPAVRALGATFGPLLLRAPPPPSPPPGGAPDGTEPTPDFPALLVEKLLQEHLEEQEVAPPALPPKPPKTKPAPTGLANGGSPPSLQDAEWYWGDISREEVNEKLRDTPDGTFLVRDASSKIQGEYTLTLRKGGNNKLIKVFHRDGHYGFSEPLTFCSVVDLITHYRHESLAQYNAKLDTRLLYPVSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQELRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQYSLMEDEDDLPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPPPAAR DIP protein P23726 original sequence version: 125 9D2BA8B6DB087098 ifih1_human Interferon-induced helicase C domain-containing protein 1 Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated protein 5 Murabutide down-regulated protein RNA helicase-DEAD box protein 116 RIG-I-like receptor 2 IFIH1 MDA5 RH116 Clinically amyopathic dermatomyositis autoantigen 140 kDa protein protein human Homo sapiens Human MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENSLLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTASPGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTRIQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED DIP protein Q9BYX4 original sequence version: 130 DIP protein Q9BYX4 original sequence version: 120 DIP protein Q9BYX4 original sequence version: 123 DIP protein Q9BYX4 original sequence version: 66 DIP protein Q9BYX4 original sequence version: 82 DIP protein Q9BYX4-1 original sequence version: 136 DIP protein Q9BYX4-1 original sequence version: 107 DIP protein Q9BYX4 original sequence version: 122 789CFB4824B92DC9 p85b_human Phosphatidylinositol 3-kinase regulatory subunit beta PIK3R2 Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta protein protein human Homo sapiens Human MAGPEGFQYRALYPFRRERPEDLELLPGDVLVVSRAALQALGVAEGGERCPQSVGWMPGLNERTRQRGDFPGTYVEFLGPVALARPGPRPRGPRPLPARPRDGAPEPGLTLPDLPEQFSPPDVAPPLLVKLVEAIERTGLDSESHYRPELPAPRTDWSLSDVDQWDTAALADGIKSFLLALPAPLVTPEASAEARRALREAAGPVGPALEPPTLPLHRALTLRFLLQHLGRVASRAPALGPAVRALGATFGPLLLRAPPPPSSPPPGGAPDGSEPSPDFPALLVEKLLQEHLEEQEVAPPALPPKPPKAKPASTVLANGGSPPSLQDAEWYWGDISREEVNEKLRDTPDGTFLVRDASSKIQGEYTLTLRKGGNNKLIKVFHRDGHYGFSEPLTFCSVVDLINHYRHESLAQYNAKLDTRLLYPVSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQYALMEDEDDLPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPPPAAR DIP protein O00459 original sequence version: 151 DIP protein O00459 original sequence version: 158 ADAC3E4B61F3F44A p/v/c-ifih1-1 2 13 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association ns-pik3r2-1 6 8 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 14 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association ns-pik3r2-2 1 10 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association ns-pik3r2-8 5 8 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P03496:p.Tyr89Phe Y89F mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y F This feature has been corrected as a result of our quality control procedures. The original label was 'Y89F'. Wed May 02 20:00:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 19:07:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' P03496:p.Met93Ala M93A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M93A'. Wed May 02 15:37:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met93ala' Tue Jun 30 15:43:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met93Ala' human Homo sapiens Human 14 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association ns-pik3r2-9 7 9 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 14 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association ns-pik3r2-10 3 8 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 14 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association ns-pik3r2-11 4 8 unspecified role unspecified role bait bait P03496:p.Tyr89Phe Y89F mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y F This feature has been corrected as a result of our quality control procedures. The original label was 'Y89F'. Wed May 02 17:30:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 20:16:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association