DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. Structure (London, England : 1993) 2006 imex curation Van Wagoner RM., Clardy J. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Van Wagoner RM., Clardy J. DIP original experiment: DIP-66366X Structure (London, England : 1993) 2006 IMEx FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. Structure (London, England : 1993) 2006 imex curation Van Wagoner RM., Clardy J. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Van Wagoner RM., Clardy J. DIP original experiment: DIP-66364X Structure (London, England : 1993) 2006 IMEx FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. Structure (London, England : 1993) 2006 imex curation Van Wagoner RM., Clardy J. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Van Wagoner RM., Clardy J. DIP original experiment: DIP-66365X Structure (London, England : 1993) 2006 IMEx FeeM, an N-acyl amino acid synthase from an uncultured soil microbe: structure, mechanism, and acyl carrier protein binding. q8knz8_9bact protein protein uncultured bacterium cslc2 uncultured bacterium cslc2 MTYHDMDSRDTIIEIIADTLQVPREQVREESKLIDLAKDSIALFELLIRLEKQFSHKVKYEDIAHIETVGDISAFIGALPSATVLPLSQESVA B33F762EDB9D9E26 q8knz7_9bact protein protein uncultured bacterium cslc2 uncultured bacterium cslc2 MTPRKVARILVAPNERDAARRIVRTTYEAQGYAIDESFATFLEGPSATTFGLFNGEVLYGTISIINDGAQGLPMDSIYAVELAAWRGEGKKLAEVVQFAMDHTLYEAVAGAKPSPFEAASLFTMVLTYALETHIDYLCISINPKHDTFYSLLGFTQIGALKHYGTVNAPAIARALYVPEWRSQTLLAQFMDAERSH 9707700FFB3EEB65 feem-1 2 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction feem-2 3 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction feel-feem-1 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro 5 enzyme enzyme unspecified role unspecified role Q8KNZ7:p.Phe148Asp F148D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F D This feature has been corrected as a result of our quality control procedures. The original label was 'F148D'. Wed May 02 20:17:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe148asp' Tue Jun 30 17:22:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe148Asp' Q8KNZ7:p.His145Glu H145E, N, R mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H E Wed May 02 16:20:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his145glu' Tue Jun 30 17:26:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His145Glu' Q8KNZ7:p.His145Asn mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H N Wed May 02 14:54:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his145asn' Tue Jun 30 17:45:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His145Asn' Q8KNZ7:p.His145Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H R Wed May 02 15:23:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his145arg' Tue Jun 30 16:13:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His145Arg' ecolx Escherichia coli enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction