DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. DIP original experiment: DIP-263774X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. DIP original experiment: DIP-263778X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. DIP original experiment: DIP-263776X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. DIP original experiment: DIP-263777X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. DIP original experiment: DIP-263773X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. The Salmonella effector protein PipB2 is a linker for kinesin-1. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Henry T., Couillault C., Rockenfeller P., Boucrot E., Dumont A., Schroeder N., Hermant A., Knodler LA., Lecine P., Steele-Mortimer O., Borg JP., Gorvel JP., Méresse S. DIP original experiment: DIP-263775X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx The Salmonella effector protein PipB2 is a linker for kinesin-1. kinh_human Kinesin-1 heavy chain KIF5B KNS KNS1 Ubiquitous kinesin heavy chain Conventional kinesin heavy chain protein protein human Homo sapiens Human MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV DIP protein P33176 original sequence version: 148 DIP protein P33176 original sequence version: 65 DIP protein P33176 original sequence version: 133 DIP protein P33176 original sequence version: 102 DIP protein P33176 original sequence version: 152 A1FE5760C3250C8B pipb2_salty Secreted effector protein PipB2 Type III effector PipB2 STM2780 pipB2 protein protein salty Salmonella typhimurium MERSLDSLAGMAKSAFGAGTSAAMRQATSPKTILEYIINFFTCGGIRRRNETQYQELIETMAETLKSTMPDRGAPLPENIILDDMDGCRVEFNLPGENNEAGQVIVRVSKGDHSETREIPLASFEKICRALLFRCEFSLPQDSVILTAQGGMNLKGAVLTGANLTSENLCDADLSGANLEGAVLFMADCEGANFKGANLSGTSLGDSNFKNACLEDSIMCGATLDHANLTGANLQHASLLGCSMIECNCSGANMDHTNLSGATLIRADMSGATLQGATIMAAIMEGAVLTRANLRKASFISTNLDGADLAEANLNNTCFKDCTLTDLRTEDATMSTSTQTLFNEFYSENI DIP protein Q8ZMM8 original sequence version: 64 klc1_human Kinesin light chain 1 KLC1 KLC KNS2 protein protein human Homo sapiens Human MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNGGVSGRASFCGKRQQQQWPGRRHR DIP protein Q07866 original sequence version: 146 A66E3915C57C2764 klc2_mouse Kinesin light chain 2 Klc2 protein protein mouse Mus musculus Mouse MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEMLPQEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDRRPMEYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGRGHRRGSRDVAGPQSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPNSRMKRASSLNFLNKSVEEPVQPGGRVFLTAAL DIP protein O88448 original sequence version: 114 DIP protein O88448 original sequence version: 80 A5E6F9081609E1CE pipb2-klc1-1 5 9 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association pipb2-kif5b-1 2 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association pipb2-klc1-2 3 9 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association pipb2-klc1-3 1 9 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association pipb2-klc1-4 4 9 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction pipb2-klc2-1 6 10 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association