DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Nature structural & molecular biology 2006 imex curation Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. DIP original experiment: DIP-66522X Nature structural & molecular biology 2006 IMEx Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Nature structural & molecular biology 2006 imex curation Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. DIP original experiment: DIP-66525X Nature structural & molecular biology 2006 IMEx Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Nature structural & molecular biology 2006 imex curation Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. DIP original experiment: DIP-66521X Nature structural & molecular biology 2006 IMEx Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Nature structural & molecular biology 2006 imex curation Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. DIP original experiment: DIP-66520X Nature structural & molecular biology 2006 IMEx Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Nature structural & molecular biology 2006 imex curation Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. DIP original experiment: DIP-66524X Nature structural & molecular biology 2006 IMEx Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. Nature structural & molecular biology 2006 imex curation Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Houtman JC., Yamaguchi H., Barda-Saad M., Braiman A., Bowden B., Appella E., Schuck P., Samelson LE. DIP original experiment: DIP-66523X Nature structural & molecular biology 2006 IMEx Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. sos1_mouse Son of sevenless homolog 1 Sos1 protein protein mouse Mus musculus Mouse MQAQQLPYEFFSEENAPKWRGLLVPALKKVQGQVHPTLESNDDALQYVEELILQLLNMLCQAQPRSASDVEERVQKSFPHPIDKWAIADAQSAIEKRKRRNPLSLPAERIHHLLREVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKAFMAEIRQYIRELNLIIKVFREPFVSNSKLFSSNDVENIFSRIVDIHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYARDILRPGFHGHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECMKQAITALLNVQSGMEKICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASSAEYRLKEKFFMRKVQINDKDDTSEYKHAFEIILKDGNSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTVLQEEKEEQMRLPSAEVYRFAEPDSEENILFEENVQPKAGIPIIKAGTVLKLIERLTYHMYADPNFVRTFLTTYRSFCRPQELLSLLIERFEIPEPEPTEADRIAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDADLLQRMEEFIGTVRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVETENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLRRHGKELINFSKRRRVAEITGEIQQYQNQPYCLRVEPDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRHPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESTASAPNSPRTPLTPPPASGTSSNTDVCSVFDSDHSASPFHSRSASVSSISLSKGTDEVPVPPPVPPRRRPESAPAESSPSKIMSKHLDSPPAIPPRQPTSKAYSPRYSISDRTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSDHGNAFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQEMDLHSIAGPPVPPRQSTSQLIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS 3286088A5BA0A4A6 3286088A5BA0A4A6 p62993-1 Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 Protein Ash SH2/SH3 adapter GRB2 Ash-L protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV DIP protein P62993-1 original sequence version: 88 DIP protein P62993 original sequence version: 100 DIP protein P62993 original sequence version: 132 DIP protein P62993 original sequence version: 154 DIP protein P62993-1 original sequence version: 45 o43561-2 Linker for activation of T-cells family member 1 36 kDa phospho-tyrosine adapter protein p36-38 LAT Short protein protein human Homo sapiens Human MEEAILVPCVLGLLLLPILAMLMALCVHCHRLPGSYDSTSSDSLYPRGIQFKRPHTVAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANSVASYENEEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTPGIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTEPAALSSQEAEEVEEEGAPDYENLQELN DIP protein O43561-2 original sequence version: 110 DIP protein O43561-2 original sequence version: 58 mint 0832E2D2B4220BC6 cbl_human E3 ubiquitin-protein ligase CBL Signal transduction protein CBL Proto-oncogene c-Cbl Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 CBL CBL2 RNF55 RING-type E3 ubiquitin transferase CBL protein protein human Homo sapiens Human MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT DIP protein P22681 original sequence version: 173 DIP protein P22681 original sequence version: 128 DIP protein P22681 original sequence version: 145 DIP protein P22681 original sequence version: 150 DIP protein P22681 original sequence version: 191 DIP protein P22681 original sequence version: 91 DIP protein P22681 original sequence version: 139 1AA6BF67377322CA cbl-grb2-7 3 10 unspecified role unspecified role neutral component neutral component 461-700 binding region 461-700 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction grb2-lat-4 5 9 unspecified role unspecified role unspecified role unspecified role 191Y-P 191Y-P phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role 1117-1319 binding region 1117-1319 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association grb2-sos1-11 4 7 unspecified role unspecified role neutral component neutral component 1117-1213 binding region 1117-1213 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 1214-1319 binding region 1214-1319 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction grb2-sos1-12 1 7 unspecified role unspecified role neutral component neutral component 1117-1319 binding region 1117-1319 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 1117-1213 binding region 1117-1213 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction grb2-lat-5 6 9 unspecified role unspecified role unspecified role unspecified role 164-233 binding region 164-233 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 171Y-P 171Y-P optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 191Y-P 191Y-P optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 226Y-P 226Y-P optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction grb2-lat-6 2 9 unspecified role unspecified role neutral component neutral component 164-233 binding region 164-233 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 191Y-P 191Y-P phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 226Y-P 226Y-P phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain 171Y-P 171Y-P phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli direct interaction direct interaction