DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Li L., Kim BG., Cheong YH., Pandey GK., Luan S. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Li L., Kim BG., Cheong YH., Pandey GK., Luan S. DIP original experiment: DIP-263520X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Li L., Kim BG., Cheong YH., Pandey GK., Luan S. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Li L., Kim BG., Cheong YH., Pandey GK., Luan S. DIP original experiment: DIP-263519X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Li L., Kim BG., Cheong YH., Pandey GK., Luan S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Li L., Kim BG., Cheong YH., Pandey GK., Luan S. DIP original experiment: DIP-263518X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx A Ca(2)+ signaling pathway regulates a K(+) channel for low-K response in Arabidopsis. akt1_arath Potassium channel AKT1 AKT1 At2g26650 F18A8.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRGGALLCGQVQDEIEQLSRESSHFSLSTGILPSLGARSNRRVKLRRFVVSPYDHKYRIWEAFLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLIVDDRKQIAFKYLRSWFLLDLVSTIPSEAAMRISSQSYGLFNMLRLWRLRRVGALFARLEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLWMRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTSRTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSSISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTADLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQANVGDGTIIMNNLLQHLKEMNDPVMTNVLLEIENMLARGKMDLPLNLCFAAIREDDLLLHQLLKRGLDPNESDNNGRTPLHIAASKGTLNCVLLLLEYHADPNCRDAEGSVPLWEAMVEGHEKVVKVLLEHGSTIDAGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAVCEENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKALFREKLHERRVHIETSSSVPILKTGIRFLGRFTSEPNIRPASREVSFRIRETRARRKTNNFDNSLFGILANQSVPKNGLATVDEGRTGNPVRVTISCAEKDDIAGKLVLLPGSFKELLELGSNKFGIVATKVMNKDNNAEIDDVDVIRDGDHLIFATDS DIP protein Q38998 original sequence version: 122 DIP protein Q38998 original sequence version: 69 28E1622BA3505F4C cipkn_arath CBL-interacting serine/threonine-protein kinase 23 LKS1 PKS17 SnRK3.23 SOS2-like protein kinase PKS17 SNF1-related kinase 3.23 Protein LOW-K(+)-SENSITIVE 1 F12P21.6 CIPK23 At1g30270 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASRTTPSRSTPSRSTPSGSSSGGRTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKREISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDKISSNGRLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVREDGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKIFKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWFKKGYKAPKFENADVSLDDVDAIFDDSGESKNLVVERREEGLKTPVTMNAFELISTSQGLNLGSLFEKQMGLVKRKTRFTSKSSANEIVTKIEAAAAPMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYKNLTTGLKDIVWKTIDEEKEEGTDGGGTNGAMANRTIAKQST DIP protein Q93VD3 original sequence version: 98 DIP protein Q93VD3 original sequence version: 45 CAA1B526B955F5F2 cipk23-akt1-3 3 4 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association akt1-cipk23-1 2 4 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 287-857 Region 287-857 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation cipk23-1 1 5 putative self putative self unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true