DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. DIP original experiment: DIP-263352X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. DIP original experiment: DIP-263350X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. DIP original experiment: DIP-263353X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Whirlin complexes with p55 at the stereocilia tip during hair cell development. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Mburu P., Kikkawa Y., Townsend S., Romero R., Yonekawa H., Brown SD. DIP original experiment: DIP-263351X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Whirlin complexes with p55 at the stereocilia tip during hair cell development. whrn_mouse Whirlin Whrn Dfnb31 Kiaa1526 protein protein mouse Mus musculus Mouse MNAQLDGLSVSSSSTGSLGSAAAAAGGGGGAGLRLLSANVRQLHQALTALLSEPEREQFTHCLNAYHARRNVFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWAAPDGAGPGEVRLVSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALKGSKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSTSPPSSLPQPHGSTLRQREDDRRSTLHLLQSGDEKKVNLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAESSGLKVGDQILEVNGRSFLNILHDEAVKLLKSSRHLILTVKDVGRLPHARTTVDQTKWIASSRIGESVANSAGFPGDHTEEGTSKPGFYKGPAGSQVTLSSLGNQTRALLDDQARHLLTEQERATMMYYLAQYRGGTISVEAMVMALFELLNTHAKFSLLSEVRSIISPQDLDRFDHLVLRREIESMKARQPPGPGVGDTYSMVSYSDTGSSTGSHGTSTTVSSARERLLWLIDLMENTLDLEGTGETTQGSTNALPDVSVDDVKSPSEDLPGIKPPPPPPPLAQGHDRLLGQPRKPGREDPAPLSSAAHSGIVFSAPRNRSPPPGTAPTPGPSSAQDSPSSPIYASISHANPSSRKPLDTHLALVNQHPIGPFPRVQSPPHLKSPPAETPGAGACLPPPSPSEHPDAVGANQHFVLVEVHRPDSEPDVNEVRALPQTRTASTLSQLSDSGQTLSEDSGVDAGETEASTSGRGRQTASAKNKNGKEQPRTERTAEGANKPPGLLEPTSTLVRVRKSAATLGIAIEGGANTRQPLPRIVTIQRGGSAHNCGQLKVGHVILEVNGQTLRGKEHKEAARIIAEAFKTKERDYIDFLVTEFNVML DIP protein Q80VW5 original sequence version: 94 Necessary for elongation and maintenance of inner and outer hair cell stereocilia in the organ of Corti in the inner ear mint Defects in Whrn are the cause of the phenotype whirler (wi). Mutants are characterized by deafness due to malformation of the cochlear inner and outer hair cells and by circling behavior. Stereocilia are shorter and wider than in wild-type animals and there is a decrease in the number of actin filaments in inner and outer hair cells. The number of outer hair cell stereocilia is reduced with increased spacing between them 7D5EA44DE0645AA4 em55_mouse 55 kDa erythrocyte membrane protein Mpp1 Membrane protein, palmitoylated 1 protein protein mouse Mus musculus Mouse MTLKSSEGEGGNSMRTALSDLYLEHLLQKRNRPETSLNQSNVTTEDMYTNGSPAPGSPAHAKGQEARRVRLIQFEKITEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIANQQSRLPALQMFMRAQFDYDPQKDNLIPCKEAGLKFVTGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASVAHSAPSEAPSCSPFGKKKKCKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNGLLSHNPEKFAYPAPYTTRPPKKSEEDGKEYHFISTEEMTKNISANEFLEFGSYQGNMFGTKFETVHQIHKQDKIAILDIEPQTLKTVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNSVDETLKKLQEAFDQACSSPQWVPVSWVY DIP protein P70290 original sequence version: 127 mint 8724DE75A717A464 whrn-mpp1-1 2 6 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association whrn-mpp1-2 4 6 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction mpp1-whrn-1 3 6 unspecified role unspecified role bait bait mouse Mus musculus Mouse 5 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association mpp1-whrn-2 1 6 unspecified role unspecified role bait bait Region 323-466 Region 323-466 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction