DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular basis for PKR activation by PACT or dsRNA. Molecular basis for PKR activation by PACT or dsRNA. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Li S., Peters GA., Ding K., Zhang X., Qin J., Sen GC. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li S., Peters GA., Ding K., Zhang X., Qin J., Sen GC. DIP original experiment: DIP-263221X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Molecular basis for PKR activation by PACT or dsRNA. Molecular basis for PKR activation by PACT or dsRNA. Molecular basis for PKR activation by PACT or dsRNA. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Li S., Peters GA., Ding K., Zhang X., Qin J., Sen GC. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Li S., Peters GA., Ding K., Zhang X., Qin J., Sen GC. DIP original experiment: DIP-263222X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Molecular basis for PKR activation by PACT or dsRNA. Molecular basis for PKR activation by PACT or dsRNA. Molecular basis for PKR activation by PACT or dsRNA. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Li S., Peters GA., Ding K., Zhang X., Qin J., Sen GC. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li S., Peters GA., Ding K., Zhang X., Qin J., Sen GC. DIP original experiment: DIP-263223X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Molecular basis for PKR activation by PACT or dsRNA. e2ak2_human Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase EIF2AK2 PKR PRKR Tyrosine-protein kinase EIF2AK2 protein protein human Homo sapiens Human MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC DIP protein P19525 original sequence version: 164 DIP protein P19525-1 original sequence version: 177 DIP protein P19525-1 original sequence version: 178 DIP protein P19525-1 original sequence version: 170 DIP protein P19525 original sequence version: 153 815AD83ACAB45DA3 o75569-1 Interferon-inducible double-stranded RNA-dependent protein kinase activator A PRKRA PACT RAX HSD-14 HSD14 PKR-associated protein X PKR-associating protein X Protein activator of the interferon-induced protein kinase Protein kinase, interferon-inducible double-stranded RNA-dependent activator protein protein human Homo sapiens Human MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK DIP protein O75569-1 original sequence version: 131 prkra-eif2ak2-3 1 5 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 4 unspecified role unspecified role prey prey Region 328-551 Region 328-551 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P19525:p.Asp328Ala D328A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D328A'. Wed May 02 16:33:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp328ala' Tue Jun 30 19:46:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp328Ala' P19525:p.Gly329Ala G329A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain G A This feature has been corrected as a result of our quality control procedures. The original label was 'G329A'. Wed May 02 15:09:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly329ala' Tue Jun 30 17:22:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly329Ala' P19525:p.Asp331Ala D331A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D331A'. Wed May 02 15:01:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp331ala' Tue Jun 30 19:55:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp331Ala' P19525:p.Tyr332Ala Y332A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y332A'. Wed May 02 17:38:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr332ala' Tue Jun 30 20:22:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr332Ala' P19525:p.Asp333Ala D333A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D333A'. Wed May 02 18:15:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp333ala' Tue Jun 30 19:34:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp333Ala' V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association prkra-eif2ak2-4 3 5 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 240-308 Region 240-308 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role prey prey Region 328-551 Region 328-551 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P19525:p.Asp328Ala D328A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D328A'. Wed May 02 17:23:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp328ala' Tue Jun 30 18:55:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp328Ala' P19525:p.Gly329Ala G329A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain G A This feature has been corrected as a result of our quality control procedures. The original label was 'G329A'. Wed May 02 14:25:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly329ala' Tue Jun 30 19:53:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly329Ala' P19525:p.Asp331Ala D331A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D331A'. Wed May 02 16:44:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp331ala' Tue Jun 30 18:40:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp331Ala' P19525:p.Tyr332Ala Y332A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y332A'. Wed May 02 18:35:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr332ala' Tue Jun 30 15:58:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr332Ala' P19525:p.Asp333Ala D333A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D333A'. Wed May 02 14:53:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp333ala' Tue Jun 30 20:02:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp333Ala' Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association eif2ak2-prkra-1 2 5 unspecified role unspecified role unspecified role unspecified role Region 240-308 Region 240-308 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Region 326-337 Region 326-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction