DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". Structure (London, England : 1993) 2006 imex curation Pullan L., Mullapudi S., Huang Z., Baldwin PR., Chin C., Sun W., Tsujimoto S., Kolodziej SJ., Stoops JK., Lee JC., Waxham MN., Bean AJ., Penczek PA. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Pullan L., Mullapudi S., Huang Z., Baldwin PR., Chin C., Sun W., Tsujimoto S., Kolodziej SJ., Stoops JK., Lee JC., Waxham MN., Bean AJ., Penczek PA. DIP original experiment: DIP-66287X Structure (London, England : 1993) 2006 IMEx The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". Structure (London, England : 1993) 2006 imex curation Pullan L., Mullapudi S., Huang Z., Baldwin PR., Chin C., Sun W., Tsujimoto S., Kolodziej SJ., Stoops JK., Lee JC., Waxham MN., Bean AJ., Penczek PA. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pullan L., Mullapudi S., Huang Z., Baldwin PR., Chin C., Sun W., Tsujimoto S., Kolodziej SJ., Stoops JK., Lee JC., Waxham MN., Bean AJ., Penczek PA. DIP original experiment: DIP-66288X Structure (London, England : 1993) 2006 IMEx The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". Structure (London, England : 1993) 2006 imex curation Pullan L., Mullapudi S., Huang Z., Baldwin PR., Chin C., Sun W., Tsujimoto S., Kolodziej SJ., Stoops JK., Lee JC., Waxham MN., Bean AJ., Penczek PA. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Pullan L., Mullapudi S., Huang Z., Baldwin PR., Chin C., Sun W., Tsujimoto S., Kolodziej SJ., Stoops JK., Lee JC., Waxham MN., Bean AJ., Penczek PA. DIP original experiment: DIP-66286X Structure (London, England : 1993) 2006 IMEx The endosome-associated protein Hrs is hexameric and controls cargo sorting as a "master molecule". snp25_mouse Synaptosomal-associated protein 25 Synaptosomal-associated 25 kDa protein Super protein Snap25 Snap protein protein mouse Mus musculus Mouse MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG DIP protein P60879 original sequence version: 38 DIP protein P60879-1 original sequence version: 41 DIP protein P60879-1 original sequence version: 109 DIP protein P60879-1 original sequence version: 81 DIP protein P60879 original sequence version: 111 FBED2B082A4CB6A6 hgs_mouse Hepatocyte growth factor-regulated tyrosine kinase substrate Hgs Hrs protein protein mouse Mus musculus Mouse MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKAEGKASSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERMRQKTTYTAHPKAEPTPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMAEAPLPETDSQPITPCSGPFSEYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINTMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPTAGGVLYQPSGPTSFPATFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMVSAYMYPTGAPGAQAAPQAQAGPTTSPAYSSYQPTPTPGYQSVASQAPQSLPAISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTALPGQDASLPAQQPYIPGQQPLYQQMAPSTGPPQQQPPVAQPAPTQGPPAQGSEAQLISFD DIP protein Q99LI8 original sequence version: 38 DIP protein Q99LI8 original sequence version: 96 DIP protein Q99LI8 original sequence version: 90 DIP protein Q99LI8 original sequence version: 97 DIP protein Q99LI8 original sequence version: 76 0E68BF5AB514865B hgs-1 3 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction snap25-hgs-1 1 5 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role bait bait GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range mouse Mus musculus Mouse direct interaction direct interaction hgs-2 2 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction