DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Nature 2006 imex curation Darios F., Davletov B. Only protein-protein interactions rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Darios F., Davletov B. DIP original experiment: DIP-255196X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Nature 2006 imex curation Darios F., Davletov B. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Darios F., Davletov B. DIP original experiment: DIP-255194X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Nature 2006 imex curation Darios F., Davletov B. Only protein-protein interactions in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Darios F., Davletov B. DIP original experiment: DIP-255195X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Nature 2006 imex curation Darios F., Davletov B. Only protein-protein interactions rat Rattus norvegicus (Rat) Rat anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Darios F., Davletov B. DIP original experiment: DIP-255197X Nature 2006 IMEx Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG DIP protein P60881 original sequence version: 105 DIP protein P60881 original sequence version: 40 DIP protein P60881 original sequence version: 106 DIP protein P60881 original sequence version: 78 DIP protein P60881 original sequence version: 75 DIP protein P60881 original sequence version: 100 DIP protein P60881 original sequence version: 120 DIP protein P60881 original sequence version: 131 DIP protein P60881 original sequence version: 93 FBED2B082A4CB6A6 stx3_rat Syntaxin-3 Stx3 Stx3a protein protein rat Rattus norvegicus (Rat) Rat MKDRLEQLKAKQLTQDDDTDEVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKRAMKYQGQARKKLIIIIVIVVVLLGILALIIGLSVGLK DIP protein Q08849 original sequence version: 94 mint Potentially involved in docking of synaptic vesicles at presynaptic active zones 4A8EAFD4684EEE6F stx3-snap25-1 1 6 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat 5 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat physical association physical association snap25-stx3-1 2 6 unspecified role unspecified role unspecified role unspecified role Region 1-261 Region 1-261 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction snap25-stx3-2 4 6 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat 5 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat physical association physical association stx3-snap25-2 3 6 unspecified role unspecified role bait bait Region 1-261 Region 1-261 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction