IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rapid production of human KIR2DL4 extracellular domain and verification of its interaction with HLA-G. Rapid production of human KIR2DL4 extracellular domain and verification of its interaction with HLA-G. Biochemistry. Biokhimiia 2005 imex curation Yu YR., Tian XH., Wang Y., Feng MF. there are multitudinous versions of the KIR2DL protein - sequence given in fig 2 is a little shorter than canonical The variant of KIR2DL4 (GenBank No. AY601812) used here has 397 aas and is not listed in UniProt. It is stated to have an extra fragment in its cytoplasm domain that encodes 21 amino acid residues. The N-terminal region sequence also differs from other KIR2DL4 protein entries. Apart from the N-terminal ~8 amino acids and the extra 21 aas in the cytoplasmic domain, the protein appears to have a similar sequence to the UniProt canonical entry for KIR2DL4, notably Q99706-1. N and C termini of the ECD were determined from primers. yuyr@ioz.ac.cn,fengmf@ioz.ac.cn 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yu YR., Tian XH., Wang Y., Feng MF. there are multitudinous versions of the KIR2DL protein - sequence given in fig 2 is a little shorter than canonical The variant of KIR2DL4 (GenBank No. AY601812) used here has 397 aas and is not listed in UniProt. It is stated to have an extra fragment in its cytoplasm domain that encodes 21 amino acid residues. The N-terminal region sequence also differs from other KIR2DL4 protein entries. Apart from the N-terminal ~8 amino acids and the extra 21 aas in the cytoplasmic domain, the protein appears to have a similar sequence to the UniProt canonical entry for KIR2DL4, notably Q99706-1. N and C termini of the ECD were determined from primers. yuyr@ioz.ac.cn,fengmf@ioz.ac.cn Accepted 2017-NOV-08 AT 16:48 GMT AT 16:48 GMT by PPORRAS Biochemistry. Biokhimiia 2005 Only protein-protein interactions imex curation imex curation kir2dl4_variant KIR2DL4_variant protein protein human Homo sapiens Human MLLMVVSMACLGFFLDQSVWAHVGGQDKPFCSAWPSAVVPQGGHVTLRCHYRRGFNIFTLYKKDGVPVPELYNRIFWNSFLISPVTPAHAGTYRCRGFHPHSPTEWSAPSNPLVIMVTGLYEKPSLTARPGPTVRTGENVTLSCSSQSSFDIYHLSREGEAHELRLPAVPSINGTFQADFPLGPATHGETYRCFGSFHGSPYEWSDASDPLPVSVTGNPSSSWPSPTEPSFKTGIARHLHAVIRYSVAIILFTILPFFLLHRWCSKKKVSLTKQRPENSGPCAEAGWEHADAAVMNQEPAGHRTVNREDSDEQDPQEVTYAQLDHCIFTQRKITGPSQRSKRPSTDTSVCIELPNAEPRALSPAHEHHSQALMGSSRETTALSQTQLASSNVPAAGI p17693-1 HLA class I histocompatibility antigen, alpha chain G HLA G antigen MHC class I antigen G HLA-G1 HLA-G HLA-6.0 HLAG protein protein human Homo sapiens Human MVVMAPRTLFLLLSGALTLTETWAGSHSMRYFSAAVSRPGRGEPRFIAMGYVDDTQFVRFDSDSACPRMEPRAPWVEQEGPEYWEEETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDGKDYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQRADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQSSLPTIPIMGIVAGLVVLAAVVTGAAVAAVLWRKKSSD 0678E263BE8962FF kir2dl4_variant-hla_g-1 1 3 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells 2 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association fig 8