DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Nature structural & molecular biology 2006 imex curation Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66178X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Nature structural & molecular biology 2006 imex curation Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66179X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Nature structural & molecular biology 2006 imex curation Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66181X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. Nature structural & molecular biology 2006 imex curation Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hirano S., Kawasaki M., Ura H., Kato R., Raiborg C., Stenmark H., Wakatsuki S. DIP original experiment: DIP-66180X Nature structural & molecular biology 2006 IMEx Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting. dnjb2_human DnaJ homolog subfamily B member 2 DNAJB2 HSJ1 HSPF3 Heat shock 40 kDa protein 3 Heat shock protein J1 protein protein human Homo sapiens Human MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL DIP protein P25686 original sequence version: 62 0154ED3E29F34B4A ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 eps15_mouse Epidermal growth factor receptor substrate 15 Protein AF-1p Eps15 protein protein mouse Mus musculus Mouse MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLKKSGLPDLILGKIWDLADTDGKGVLSKQEFFVALRLVACAQNGLEVSLSSLSLAVPPPRFHDSSSPLLTSGPSVAELPWAVKSEDKAKYDAIFDSLSPVDGFLSGDKVKPVLLNSKLPVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSALLAHIWSLCDTKGCGKLSKDQFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNITGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTVKQRTSEVQDLQDEVQRESINLQKLQAQKQQVQELLGELDEQKAQLEEQLQEVRKKCAEEAQLISSLKAEITSQESQISSYEEELLKAREELSRLQQETAQLEESVESGKAQLEPLQQHLQESQQEISSMQMRLEMKDLETDNNQSNWSSSPQSVLVNGATDYCSLSTSSSETANFNEHAEGQNNLESEPTHQESSVRSSPEIAPSDVTDESEAVTVAGNEKVTPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFTTSSTDPFSASSNSSNTSVETWKHNDPFAPGGTVVAAASDSATDPFASVFGNESFGDGFADFSTLSKVNNEDAFNPTISSSTSSVTIAKPMLEETASKSEDVPPALPPKVGTPTRPCPPPPGKRPINKLDSSDPLKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA DIP protein P42567 original sequence version: 63 08A0C0D423F873C2 hgs_human Hepatocyte growth factor-regulated tyrosine kinase substrate Protein pp110 Hrs HGS HRS protein protein human Homo sapiens Human MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKDLLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPSGGPFSEPQFHNGESEESHEQFLKALQNAVTTFVNRMKSNHMRGRSITNDSAVLSLFQSINGMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPAAGGVLYQPSGPASFPSTFSPAGSVEGSPMHGVYMSQPAPAAGPYPSMPSTAADPSMVSAYMYPAGATGAQAAPQAQAGPTASPAYSSYQPTPTAGYQNVASQAPQSLPAISQPPQSSTMGYMGSQSVSMGYQPYNMQNLMTTLPSQDASLPPQQPYIAGQQPMYQQMAPSGGPPQQQPPVAQQPQAQGPPAQGSEAQLISFD DIP protein O14964 original sequence version: 96 DIP protein O14964 original sequence version: 47 DIP protein O14964 original sequence version: 88 DIP protein O14964-1 original sequence version: 88 DIP protein O14964 original sequence version: 98 DD64167A19DCF030 hgs-ubiquitin-1 1 8 unspecified role unspecified role neutral component neutral component 257-277 binding region 257-277 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role neutral component neutral component bovin Bos taurus (Bovine) Bovine direct interaction direct interaction hgs-ubiquitin-2 2 8 unspecified role unspecified role bait bait 251-286 binding region 251-286 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain O14964:p.[Ala266Gln;Ala268Gln] A266Q, A268Q mutation mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A Q certain certain sequence position certain certain certain sequence position certain A Q This feature has been corrected as a result of our quality control procedures. The original label was 'A266Q, A268Q mutation'. Wed May 02 14:52:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala266gln,ala268gln' Tue Jun 30 16:54:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ala266Gln;Ala268Gln]' GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction dnajb2-ubiquitin-1 4 5 unspecified role unspecified role bait bait 245-272 binding region 245-272 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain P25686:p.[Ala258Gln;Ala260Gln] A258Q, A260Q mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A Q certain certain sequence position certain certain certain sequence position certain A Q This feature has been corrected as a result of our quality control procedures. The original label was 'A258Q, A260Q mutation'. Wed May 02 15:19:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala258gln,ala260gln' Tue Jun 30 16:11:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ala258Gln;Ala260Gln]' GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag full dna sequence full identification by DNA sequencing full dna sequence n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction eps15-ubiquitin-1 3 7 unspecified role unspecified role bait bait 867-897 binding region 867-897 binding region sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain P42567:p.[Ala886Gln;Ala888Gln] A886Q, A888Q mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A Q certain certain sequence position certain certain certain sequence position certain A Q This feature has been corrected as a result of our quality control procedures. The original label was 'A886Q, A888Q mutation'. Wed May 02 19:59:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala886gln,ala888gln' Tue Jun 30 17:36:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ala886Gln;Ala888Gln]' GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine direct interaction direct interaction