DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Guichard A., Park JM., Cruz-Moreno B., Karin M., Bier E. Only protein-protein interactions in vitro In vitro protease assay protease assay autoradiography autoradiography Guichard A., Park JM., Cruz-Moreno B., Karin M., Bier E. DIP original experiment: DIP-262633X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Guichard A., Park JM., Cruz-Moreno B., Karin M., Bier E. Only protein-protein interactions in vitro In vitro protease assay protease assay autoradiography autoradiography Guichard A., Park JM., Cruz-Moreno B., Karin M., Bier E. DIP original experiment: DIP-262632X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Anthrax lethal factor and edema factor act on conserved targets in Drosophila. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Guichard A., Park JM., Cruz-Moreno B., Karin M., Bier E. Only protein-protein interactions in vitro In vitro protease assay protease assay autoradiography autoradiography Guichard A., Park JM., Cruz-Moreno B., Karin M., Bier E. DIP original experiment: DIP-262631X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Anthrax lethal factor and edema factor act on conserved targets in Drosophila. o62602_drome lic MKK3 p38MAPKK Dmel_CG12244 D-MKK3 DMEK3 DMKK3 DmMKK3 Dmel\CG12244 MEK3 Mek3 Mkk3 Mpk3 dMKK3 l(1)G0252 p38 CG12244 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKRHRLTPFTIAKEPEAAIVPPRNLDSRATIQIGDRTFDIDADSLEKICDLGRGAYGIVDKMRHKQTDTVLAVKRIPMTVNIREQHRLVMDLDISMRSSDCPYTVHFYGAMYREGDVWICMEVMSTSLDKFYPKVFLHDLRMEESVLGKIAMSVVSALHYLHAQLKVIHRDVKPSNILINRAGQVKICDFGISGYLVDSIAKTIDAGCKPYMAPERIDPQGNPAQYDIRSDVWSLGIGMIEMATGRYPYDNWRTPFEQLRQVVEDSPPRLPEGTFSPEFEDFIAVCLQKEYMARPNYEQLLKHSFIVEHLQRNTDISEFVARILDLPDAQPAQ DIP protein O62602 original sequence version: 131 A4D4B71B3E5BD288 mp2k3_human Dual specificity mitogen-activated protein kinase kinase 3 MAPK/ERK kinase 3 MAP2K3 MEK3 MKK3 PRKMK3 SKK2 Stress-activated protein kinase kinase 2 protein protein human Homo sapiens Human MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS DIP protein P46734 original sequence version: 154 DIP protein P46734 original sequence version: 108 A80BA4FDFF8F75A2 lef_bacan Lethal factor Anthrax lethal toxin endopeptidase component lef pXO1-107 BXA0172 GBAA_pXO1_0172 protein protein bacan Bacillus anthracis MNIKKEFIKVISMSCLVTAITLSGPVFIPLVQGAGGHGDVGMHVKEKEKNKDENKRKDEERNKTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVPSDVLEMYKAIGGKIYIVDGDITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVLVIQSSEDYVENTEKALNVYYEIGKILSRDILSKINQPYQKFLDVLNTIKNASDSDGQDLLFTNQLKEHPTDFSVEFLEQNSNEVQEVFAKAFAYYIEPQHRDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLARYEKWEKIKQHYQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSDFLSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKLDIQPYDINQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGSTLYNKIYLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMIVDINERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEIKDVQIIKQSEKEYIRIDAKVVPKSKIDTKIQEAQLNINQEWNKALGLPKYTKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNGRFVFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFIHEFGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAEFFAEAFRLMHSTDHAERLKVQKNAPKTFQFINDQIKFIINS DIP protein P15917 original sequence version: 109 DIP protein P15917 original sequence version: 138 DIP protein P15917 original sequence version: 93 DIP protein P15917 original sequence version: 98 DIP protein P15917 original sequence version: 112 DIP protein P15917 original sequence version: 125 2076B4D7277317EE hep_drome Dual specificity mitogen-activated protein kinase kinase hemipterous hep hem MKK7 CG4353 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTIEFETIGSRLQSLEAKLQAQNESHDQIVLSGARGPVVSGSVPSARVPPLATSASAATSATHAPSLGAGSVSGSGISIAQRPAPPVPHATPFGSASASSSSSSASAFASAAPATGTFGGTYTPPTTRVSRATPTLPMLSSGPGGGLNRTRPVILPLPTPPHPPVSETDMKLKIIMEQTGKLNINGRQYPTDINDLKHLGDLGNGTSGNVVKMMHLSSNTIIAVKQMRRTGNAEENKRILMDLDVVLKSHDCKYIVKCLGCFVRDPDVWICMELMSMCFDKLLKLSKKPVPEQILGKVTVATVNALSYLKDKHGVIHRDVKPSNILIDERGNIKLCDFGISGRLVDSKANTRSAGCAAYMAPERIDPKKPKYDIRADVWSLGITLVELATARSPYEGCNTDFEVLTKVLDSEPPCLPYGEGYNFSQQFRDFVIKCLTKNHQDRPKYPELLAQPFIRIYESAKVDVPNWFQSIKDNRLRANGDPTLQRATATGSAIGSGAGSLAGSGSGSAGGAVKYGRATTYAGQSPTNPQKTIKPTQIPSYQQQQSQFFMQSATQLPQTTTTTPTATTNCFGGSGNGNGRGNGSGGSGNGSGSSSSASPLSPPSAGIGDLNRLYRKSPFMQRKLSNGSHHPHYKYNDESPKKESMFSSIGQSILRNLTTSPFSQKKHNSTATTIPLPHNNQTLITDAATAAAAAATATTPPNIAATVLTTTPTTTPTWRLPTENSQAYDSCDSSSNATTTTLNLGLSSPSPSLPRKQFPTESPTLQLTSQQQQQPQRLQPGNQSPIVLQRFYHQQNQLREKEAAERYQQQRQQPPVGVTSTNPFHSNYVPPPPSTHSTSSQSSTQSTCSQIAINPASISPSSGSGTGNMAGLGIGSAPASGLGAAGHFGAGGTGEQLQYQPLPIAAEATGTSPTLQSRSPEQQSDYGGNGNMVASKLSKLYARRQLLGQSSSSGASNSSLDGCSREQHDAGWFNTLAGAMKRQFATYVKTQLNSTATSPVASSMDRDQEPVHPQPPAYRSVVNNGSGGKSYYYRTLSAASSSSNTSQSTSPTTEPLPGGGTSSFLRRYASSGPGGSISTPPSPHILAGLDRRHRSPDPPPRYNRGQSPLLLRKNLLELSGQPPGSPLLHRRYVSASPPLPPPRRGSESVPGSPQHFRTRIHYTPEPQRRIYRTIDQ DIP protein Q23977 original sequence version: 129 79A2987068EC1870 lef-map2k3-1 3 5 enzyme target enzyme target substrate substrate unspecified role unspecified role 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage hep-lef-1 2 7 enzyme target enzyme target substrate substrate unspecified role unspecified role 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage lef-lic-1 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage