IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. The Journal of biological chemistry 2006 imex curation Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) comigration in sds comigration in sds page comigration in sds predetermined predetermined participant predetermined Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) SDS-PAGE of the HLA-G under nonreducing conditions. Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp Accepted 2017-DEC-12 AT 08:26 GMT AT 08:26 GMT by ORCHARD The Journal of biological chemistry 2006 imex curation I assume you meant to add the C(42)S mutation as "disrupting" in fig 1A. I had assumed the "dimer" refers to HLA-Gx2 as B2M is sometimes stated to be present and sometimes not - even if it is present, so I have ignored B2M.; altered; altered; C(42)S added as mutation disrupting; I have requested on Git-hub a new CV term although they state "non-reducing conditions" not - non-denaturing conditions (meaning the same thing?), so in the absence of any experimental conditions in Methods section, I assume they mean electrophoresis in the absence of 2-ME -mercaptoethanol, which is standard proceedure? (so does not need a new CV term adding?) I have altered the Interaction Detection method to "comig in SDS gel" (which states "denaturing SDS gel") from higher level term "electrophoresis". Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. The Journal of biological chemistry 2006 imex curation Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by staining molecular weight estimation by staining weight by staining Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp Accepted 2017-DEC-12 AT 08:26 GMT AT 08:26 GMT by ORCHARD The Journal of biological chemistry 2006 imex curation I assume you meant to add the C(42)S mutation as "disrupting" in fig 1A. I had assumed the "dimer" refers to HLA-Gx2 as B2M is sometimes stated to be present and sometimes not - even if it is present, so I have ignored B2M.; altered; altered; C(42)S added as mutation disrupting; I have requested on Git-hub a new CV term although they state "non-reducing conditions" not - non-denaturing conditions (meaning the same thing?), so in the absence of any experimental conditions in Methods section, I assume they mean electrophoresis in the absence of 2-ME -mercaptoethanol, which is standard proceedure? (so does not need a new CV term adding?) I have altered the Interaction Detection method to "comig in SDS gel" (which states "denaturing SDS gel") from higher level term "electrophoresis". Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. The Journal of biological chemistry 2006 imex curation Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp Accepted 2017-DEC-12 AT 08:26 GMT AT 08:26 GMT by ORCHARD The Journal of biological chemistry 2006 imex curation I assume you meant to add the C(42)S mutation as "disrupting" in fig 1A. I had assumed the "dimer" refers to HLA-Gx2 as B2M is sometimes stated to be present and sometimes not - even if it is present, so I have ignored B2M.; altered; altered; C(42)S added as mutation disrupting; I have requested on Git-hub a new CV term although they state "non-reducing conditions" not - non-denaturing conditions (meaning the same thing?), so in the absence of any experimental conditions in Methods section, I assume they mean electrophoresis in the absence of 2-ME -mercaptoethanol, which is standard proceedure? (so does not need a new CV term adding?) I have altered the Interaction Detection method to "comig in SDS gel" (which states "denaturing SDS gel") from higher level term "electrophoresis". Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. The Journal of biological chemistry 2006 imex curation Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by staining molecular weight estimation by staining weight by staining Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp Accepted 2017-DEC-12 AT 08:26 GMT AT 08:26 GMT by ORCHARD The Journal of biological chemistry 2006 imex curation I assume you meant to add the C(42)S mutation as "disrupting" in fig 1A. I had assumed the "dimer" refers to HLA-Gx2 as B2M is sometimes stated to be present and sometimes not - even if it is present, so I have ignored B2M.; altered; altered; C(42)S added as mutation disrupting; I have requested on Git-hub a new CV term although they state "non-reducing conditions" not - non-denaturing conditions (meaning the same thing?), so in the absence of any experimental conditions in Methods section, I assume they mean electrophoresis in the absence of 2-ME -mercaptoethanol, which is standard proceedure? (so does not need a new CV term adding?) I have altered the Interaction Detection method to "comig in SDS gel" (which states "denaturing SDS gel") from higher level term "electrophoresis". Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. The Journal of biological chemistry 2006 imex curation Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp Accepted 2017-DEC-12 AT 08:26 GMT AT 08:26 GMT by ORCHARD The Journal of biological chemistry 2006 imex curation I assume you meant to add the C(42)S mutation as "disrupting" in fig 1A. I had assumed the "dimer" refers to HLA-Gx2 as B2M is sometimes stated to be present and sometimes not - even if it is present, so I have ignored B2M.; altered; altered; C(42)S added as mutation disrupting; I have requested on Git-hub a new CV term although they state "non-reducing conditions" not - non-denaturing conditions (meaning the same thing?), so in the absence of any experimental conditions in Methods section, I assume they mean electrophoresis in the absence of 2-ME -mercaptoethanol, which is standard proceedure? (so does not need a new CV term adding?) I have altered the Interaction Detection method to "comig in SDS gel" (which states "denaturing SDS gel") from higher level term "electrophoresis". Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer. The Journal of biological chemistry 2006 imex curation Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K. NO isoforms of HLA-G mentioned, so presumably OK to check now. (August 2017) Details of B2M and HLA-G constructs have been taken from PDB entry 2d31. kmaenaka@bioreg.kyushu-u.ac.jp Accepted 2017-DEC-12 AT 08:26 GMT AT 08:26 GMT by ORCHARD The Journal of biological chemistry 2006 imex curation I assume you meant to add the C(42)S mutation as "disrupting" in fig 1A. I had assumed the "dimer" refers to HLA-Gx2 as B2M is sometimes stated to be present and sometimes not - even if it is present, so I have ignored B2M.; altered; altered; C(42)S added as mutation disrupting; I have requested on Git-hub a new CV term although they state "non-reducing conditions" not - non-denaturing conditions (meaning the same thing?), so in the absence of any experimental conditions in Methods section, I assume they mean electrophoresis in the absence of 2-ME -mercaptoethanol, which is standard proceedure? (so does not need a new CV term adding?) I have altered the Interaction Detection method to "comig in SDS gel" (which states "denaturing SDS gel") from higher level term "electrophoresis". lirb2_human Leukocyte immunoglobulin-like receptor subfamily B member 2 LILRB2 ILT4 LIR2 MIR10 CD85 antigen-like family member D Immunoglobulin-like transcript 4 Monocyte/macrophage immunoglobulin-like receptor 10 protein protein human Homo sapiens Human MTPIVTVLICLGLSLGPRTHVQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVVLLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKDTQPEDGVEMDTRAAASEAPQDVTYAQLHSLTLRRKATEPPPSQEREPPAEPSIYATLAIH DIP protein Q8N423 original sequence version: 127 DIP protein Q8N423 original sequence version: 104 33E9737AFCAFCE2D hlag_human HLA class I histocompatibility antigen, alpha chain G HLAG HLA-G HLA-6.0 HLA G antigen MHC class I antigen G protein protein human Homo sapiens Human MVVMAPRTLFLLLSGALTLTETWAGSHSMRYFSAAVSRPGRGEPRFIAMGYVDDTQFVRFDSDSACPRMEPRAPWVEQEGPEYWEEETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDGKDYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQRADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQSSLPTIPIMGIVAGLVVLAAVVTGAAVAAVLWRKKSSD DIP protein P17693 original sequence version: 150 DIP protein P17693 original sequence version: 89 0678E263BE8962FF h2ax_human Histone H2AX H2AX H2AFX Histone H2A.X protein protein human Homo sapiens Human MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY DIP protein P16104 original sequence version: 114 DIP protein P16104 original sequence version: 110 DIP protein P16104 original sequence version: 117 DIP protein P16104 original sequence version: 133 DIP protein P16104 original sequence version: 140 DIP protein P16104 original sequence version: 113 DIP protein P16104 original sequence version: 99 D4683775C2E6C3A9 lirb1_human Leukocyte immunoglobulin-like receptor subfamily B member 1 ILT2 LIR1 MIR7 CD85 antigen-like family member J Immunoglobulin-like transcript 2 Monocyte/macrophage immunoglobulin-like receptor 7 LILRB1 protein protein human Homo sapiens Human MTPILTVLICLGLSLGPRTHVQAGHLPKPTLWAEPGSVITQGSPVTLRCQGGQETQEYRLYREKKTALWITRIPQELVKKGQFPIPSITWEHAGRYRCYYGSDTAGRSESSDPLELVVTGAYIKPTLSAQPSPVVNSGGNVILQCDSQVAFDGFSLCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPSRRWWYRCYAYDSNSPYEWSLPSDLLELLVLGVSKKPSLSVQPGPIVAPEETLTLQCGSDAGYNRFVLYKDGERDFLQLAGAQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSEWSAPSDPLDILIAGQFYDRVSLSVQPGPTVASGENVTLLCQSQGWMQTFLLTKEGAADDPWRLRSTYQSQKYQAEFPMGPVTSAHAGTYRCYGSQSSKPYLLTHPSDPLELVVSGPSGGPSSPTTGPTSTSGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVILLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKHTQPEDGVEMDTRSPHDEDPQAVTYAEVKHSRPRREMASPPSPLSGEFLDTKDRQAEEDRQMDTEAAASEAPQDVTYAQLHSLTLRREATEPPPSQEGPSPAVPSIYATLAIH 549196EA4ED2767C p61769-pro_0000018779 Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM 21 119 B6EE395752B3322D hla_g-4 3 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P17693:p.Cys66Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:42:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys66ser' Tue Jun 30 19:28:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys66Ser' ecoli Escherichia coli (strain K12) physical association physical association 1A hla_g-5 1 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P17693:p.Cys66Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:45:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys66ser' Tue Jun 30 15:52:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys66Ser' physical association physical association 1B Beta-2-microglobulin was also present on the gel. hla_g-6 4 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P17693:p.Cys66Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:53:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys66ser' Tue Jun 30 19:26:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys66Ser' physical association physical association 1C hla_g-7 2 10 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1D the full complex was composed of one HLA-G dimer and two LILRB1s (1:2 complex). hla_g-lilrb1-1 3 10 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1E The 1(dimer):2 (LILRB1) molar ratio sample showed only one complex peak and thus represented the true equilibrium complex stoichiometry. hla_g-lilrb1-2 2 7 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1F the full complex was composed of one HLA-G dimer and two LILRB2s (1:2 complex). hla_g-lilrb2-3 3 7 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1G The 1(dimer):2 (LILRB2) molar ratio sample showed only one complex peak and thus represented the true equilibrium complex stoichiometry. b2m-h2afx-1 5 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association Table 1 3.2 A RFree = 0.298 RWork = 0.235 b2m-h2afx-2 6 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 59 52 57 physical association physical association 2 (A)B/E lilrb1-b2m-1 6 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 67 62 69 physical association physical association 2 A The simple 1:1 (Langmuir) binding model was not successful at high concentrations of the dimer, but was well fitted with a bivalent analyte model. (1:2 (dimer:LILRB) complex ) affinity constant -Kd ∼6.7 nm for LILRB1, at low concentrations of dimer lilrb1-b2m-2 6 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 77 72 79 physical association physical association 2 C The simple 1:1 (Langmuir) binding model was not successful at high concentrations of the dimer, but was well fitted with a bivalent analyte model. (1:2 (dimer:LILRB) complex ) affinity constant -Kd ∼750 nm for LILRB2, at low concentrations of dimer lilrb2-b2m-1 6 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 82 89 87 physical association physical association 2 (C)/D/E lilrb1-b2m-3 6 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 92 99 97 physical association physical association Supp fig 1, Supp. table 1 lilrb1-b2m-4 6 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 106 109 102 physical association physical association Supp fig 1, Supp Table 1 lilrb2-b2m-2 6 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 119 112 117 physical association physical association Supp fig 1, Supp. table 1 lilrb2-b2m-3 6 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P17693:p.Cys66Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:05:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys66ser' Tue Jun 30 16:59:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys66Ser' ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 130 126 122 physical association physical association Supp fig 1, Supp Table 1