DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu TAF7: a possible transcription initiation check-point regulator. TAF7: a possible transcription initiation check-point regulator. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Gegonne A., Weissman JD., Zhou M., Brady JN., Singer DS. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Gegonne A., Weissman JD., Zhou M., Brady JN., Singer DS. DIP original experiment: DIP-262493X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx TAF7: a possible transcription initiation check-point regulator. TAF7: a possible transcription initiation check-point regulator. TAF7: a possible transcription initiation check-point regulator. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Gegonne A., Weissman JD., Zhou M., Brady JN., Singer DS. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gegonne A., Weissman JD., Zhou M., Brady JN., Singer DS. DIP original experiment: DIP-262494X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx TAF7: a possible transcription initiation check-point regulator. TAF7: a possible transcription initiation check-point regulator. TAF7: a possible transcription initiation check-point regulator. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Gegonne A., Weissman JD., Zhou M., Brady JN., Singer DS. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gegonne A., Weissman JD., Zhou M., Brady JN., Singer DS. DIP original experiment: DIP-262495X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx TAF7: a possible transcription initiation check-point regulator. q80uv9-1 Transcription initiation factor TFIID subunit 1 Taf1 Ccg1 Cell cycle gene 1 protein TBP-associated factor 250 kDa Transcription initiation factor TFIID 250 kDa subunit protein protein mouse Mus musculus Mouse MGPGWAGLLQDKGGGSPSVVMSDTDSDEESAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELSGSDGALVNDEGWIRSREDAVDYSDINEVAEDESRRYQQTMGSLQPLCHTDYDEDDYDADCEDIDCKLMPPPPPPPGPLKKEKDQDDITGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQDAAQSESKDGQLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEGQVQEEECSVELEVNQKSLWNYDYAPPPLPDQCLSDDEITMMAPVESKFSQSTGDTDKVMDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKMKKTEHESTIKCNIMKKLRKLEENSGVDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFTATLDDDKPWYSIFPIDNEDLVYGRWEDNIIWDAQNMPRILEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPESDFLIIRTRQGYFIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAAAGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKATVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCHQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDNNTSLSVSRDASVYQDESNLSVLDIPSATSEKQLTQEGGDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFFAIQLSESGSDSDVESGSLRPKQPRVLQENTRMGMENEESMMSYEGDGGDASRGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDTDLDSDE DIP protein Q80UV9-1 original sequence version: 85 taf7_human Transcription initiation factor TFIID subunit 7 Transcription initiation factor TFIID 55 kDa subunit RNA polymerase II TBP-associated factor subunit F TAF7 TAF2F TAFII55 protein protein human Homo sapiens Human MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDRVPLASKLVDLPCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASKKKDKDKEKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFNDLSSSSEDEDETQHQDEEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNMKGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK DIP protein Q15545 original sequence version: 127 DIP protein Q15545 original sequence version: 53 DIP protein Q15545 original sequence version: 71 DIP protein Q15545 original sequence version: 113 0AAFD3E33E3CCD6E taf1-taf7-3 1 5 unspecified role unspecified role prey prey GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction taf1-1 3 4 putative self putative self unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation true taf1-taf7-4 2 5 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 204-349 Region 204-349 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation