DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS biology 2006 imex curation Benton R., Sachse S., Michnick SW., Vosshall LB. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Benton R., Sachse S., Michnick SW., Vosshall LB. DIP original experiment: DIP-66411X PLoS biology 2006 IMEx Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS biology 2006 imex curation Benton R., Sachse S., Michnick SW., Vosshall LB. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Benton R., Sachse S., Michnick SW., Vosshall LB. DIP original experiment: DIP-66412X PLoS biology 2006 IMEx Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS biology 2006 imex curation Benton R., Sachse S., Michnick SW., Vosshall LB. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Benton R., Sachse S., Michnick SW., Vosshall LB. DIP original experiment: DIP-66413X PLoS biology 2006 IMEx Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS biology 2006 imex curation Benton R., Sachse S., Michnick SW., Vosshall LB. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Benton R., Sachse S., Michnick SW., Vosshall LB. DIP original experiment: DIP-66414X PLoS biology 2006 IMEx Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS biology 2006 imex curation Benton R., Sachse S., Michnick SW., Vosshall LB. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Benton R., Sachse S., Michnick SW., Vosshall LB. DIP original experiment: DIP-66409X PLoS biology 2006 IMEx Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS biology 2006 imex curation Benton R., Sachse S., Michnick SW., Vosshall LB. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Benton R., Sachse S., Michnick SW., Vosshall LB. DIP original experiment: DIP-66410X PLoS biology 2006 IMEx Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. or43a_drome Odorant receptor 43a Or43a AN14 DOR43B.1 dor87 Or43B.1 CG1854 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTIEDIGLVGINVRMWRHLAVLYPTPGSSWRKFAFVLPVTAMNLMQFVYLLRMWGDLPAFILNMFFFSAIFNALMRTWLVIIKRRQFEEFLGQLATLFHSILDSTDEWGRGILRRAEREARNLAILNLSASFLDIVGALVSPLFREERAHPFGLALPGVSMTSSPVYEVIYLAQLPTPLLLSMMYMPFVSLFAGLAIFGKAMLQILVHRLGQIGGEEQSEEERFQRLASCIAYHTQVMRYVWQLNKLVANIVAVEAIIFGSIICSLLFCLNIITSPTQVISIVMYILTMLYVLFTYYNRANEICLENNRVAEAVYNVPWYEAGTRFRKTLLIFLMQTQHPMEIRVGNVYPMTLAMFQSLLNASYSYFTMLRGVTGK DIP protein P81917 original sequence version: 49 gcn4_yeast General control transcription factor GCN4 ARG9 GCN4 AAS3 Amino acid biosynthesis regulatory protein AAS101 General control protein GCN4 YEL009C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER 2ED1B8E35D509578 2ED1B8E35D509578 orco_drome Odorant receptor coreceptor Orco A45 Or83b CG10609 Odorant receptor 83b protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTTSMQPSKYTGLVADLMPNIRAMKYSGLFMHNFTGGSAFMKKVYSSVHLVFLLMQFTFILVNMALNAEEVNELSGNTITTLFFTHCITKFIYLAVNQKNFYRTLNIWNQVNTHPLFAESDARYHSIALAKMRKLFFLVMLTTVASATAWTTITFFGDSVKMVVDHETNSSIPVEIPRLPIKSFYPWNASHGMFYMISFAFQIYYVLFSMIHSNLCDVMFCSWLIFACEQLQHLKGIMKPLMELSASLDTYRPNSAALFRSLSANSKSELIHNEEKDPGTDMDMSGIYSSKADWGAQFRAPSTLQSFGGNGGGGNGLVNGANPNGLTKKQEMMVRSAIKYWVERHKHVVRLVAAIGDTYGAALLLHMLTSTIKLTLLAYQATKINGVNVYAFTVVGYLGYALAQVFHFCIFGNRLIEESSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQKAMSISGAKFFTVSLDLFASVLGAVVTYFMVLVQLK DIP protein Q9VNB5 original sequence version: 46 or22a_drome Odorant receptor 22a Or22a AN11 DOR22A.1 dor53 Or22A.1 CG12193 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLSKFFPHIKEKPLSERVKSRDAFIYLDRVMWSFGWTEPENKRWILPYKLWLAFVNIVMLILLPISISIEYLHRFKTFSAGEFLSSLEIGVNMYGSSFKCAFTLIGFKKRQEAKVLLDQLDKRCLSDKERSTVHRYVAMGNFFDILYHIFYSTFVVMNFPYFLLERRHAWRMYFPYIDSDEQFYISSIAECFLMTEAIYMDLCTDVCPLISMLMARCHISLLKQRLRNLRSKPGRTEDEYLEELTECIRDHRLLLDYVDALRPVFSGTIFVQFLLIGTVLGLSMINLMFFSTFWTGVATCLFMFDVSMETFPFCYLCNMIIDDCQEMSNCLFQSDWTSADRRYKSTLVYFLHNLQQPITLTAGGVFPISMQTNLAMVKLAFSVVTVIKQFNLAERFQ DIP protein P81909 original sequence version: 47 E9D20EF06E69E883 orco-orco-1 5 9 unspecified role unspecified role unspecified role unspecified role YFP-tag (C-terminal fragment 159-239) YFP-tag (C-terminal fragment 159-239) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role YFP-tag (N-terminal fragment 1-158) YFP-tag (N-terminal fragment 1-158) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction or43a-orco-1 6 7 unspecified role unspecified role unspecified role unspecified role YFP-tag (N-terminal fragment 1-158) YFP-tag (N-terminal fragment 1-158) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role YFP-tag (C-terminal fragment 159-239) YFP-tag (C-terminal fragment 159-239) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction or43a-or43a-1 1 7 unspecified role unspecified role unspecified role unspecified role YFP-tag (C-terminal fragment 159-239) YFP-tag (C-terminal fragment 159-239) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role YFP-tag (N-terminal fragment 1-158) YFP-tag (N-terminal fragment 1-158) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association zip-zip-2 2 8 unspecified role unspecified role unspecified role unspecified role Region 235-281 Region 235-281 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain t 159-239) t 159-239) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 235-281 Region 235-281 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain YFP-tag (N-terminal fragment 1-158) YFP-tag (N-terminal fragment 1-158) yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction orco-or43a-1 3 7 unspecified role unspecified role prey prey Region 298-342 Region 298-342 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait Region 412-459 Region 412-459 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction orco-or22a-1 4 10 unspecified role unspecified role prey prey Region 305-359 Region 305-359 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait Region 412-459 Region 412-459 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction