UniProt UniProt http://www.uniprot.org The Png1-Rad23 complex regulates glycoprotein turnover. The Png1-Rad23 complex regulates glycoprotein turnover. The Journal of cell biology 2006 imex curation Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu Accepted 2017-NOV-02 AT 13:28 GMT AT 13:28 GMT by MGT The Journal of cell biology 2006 imex curation EBI-16421974 I don't understand the comment about RAd23-RPN1 - the double mutant is there. The Png1-Rad23 complex regulates glycoprotein turnover. The Png1-Rad23 complex regulates glycoprotein turnover. The Journal of cell biology 2006 imex curation Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu Accepted 2017-NOV-02 AT 13:28 GMT AT 13:28 GMT by MGT The Journal of cell biology 2006 imex curation EBI-16421974 I don't understand the comment about RAd23-RPN1 - the double mutant is there. The Png1-Rad23 complex regulates glycoprotein turnover. The Png1-Rad23 complex regulates glycoprotein turnover. The Journal of cell biology 2006 imex curation Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu Accepted 2017-NOV-02 AT 13:28 GMT AT 13:28 GMT by MGT The Journal of cell biology 2006 imex curation EBI-16421974 I don't understand the comment about RAd23-RPN1 - the double mutant is there. The Png1-Rad23 complex regulates glycoprotein turnover. The Png1-Rad23 complex regulates glycoprotein turnover. The Journal of cell biology 2006 imex curation Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim I., Ahn J., Liu C., Tanabe K., Apodaca J., Suzuki T., Rao H. raoh@uthscsa.edu Accepted 2017-NOV-02 AT 13:28 GMT AT 13:28 GMT by MGT The Journal of cell biology 2006 imex curation EBI-16421974 I don't understand the comment about RAd23-RPN1 - the double mutant is there. png1_yeast Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase PNG1 YPL096W Peptide:N-glycanase 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGEVYEKNNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLGQSQHLCTVYDNPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDYLVKELLRYFKQDFFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNCGTVEIYKCNRCGNITRFPRYNDPIKLLETRKGRCGEWCNLFTLILKSFGLDVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIYSINWNKKMSYCIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDDEIYQLACRDEQEQIELIRGKTQETKSESVSAASKSSNRGRESGSADWKAQRGEDGK 004F3E3E07C3B954 rad23_yeast UV excision repair protein RAD23 SYGP-ORF29 YEL037C RAD23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSLTFKNFKKEKVPLDLEPSNTILETKTKLAQSISCEESQIKLIYSGKVLQDSKTVSECGLKDGDQVVFMVSQKKSTKTKVTEPPIAPESATTPGRENSTEASPSTDASAAPAATAPEGSQPQEEQTATTERTESASTPGFVVGTERNETIERIMEMGYQREEVERALRAAFNNPDRAVEYLLMGIPENLRQPEPQQQTAAAAEQPSTAATTAEQPAEDDLFAQAAQGGNASSGALGTTGGATDAAQGGPPGSIGLTVEDLLSLRQVVSGNPEALAPLLENISARYPQLREHIMANPEVFVSMLLEAVGDNMQDVMEGADDMVEGEDIEVTGEAAAAGLGQGEGEGSFQVDYTPEDDQAISRLCELGFERDLVIQVYFACDKNEEAAANILFSDHAD B3F0436DAB60B833 B3F0436DAB60B833 ufd2_yeast E4 ubiquitin-protein ligase UFD2 UFD2 YDL190C D1255 Ubiquitin fusion degradation protein 2 Ubiquitin conjugation factor E4 RING-type E3 ubiquitin transferase UFD2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTAIEDILQITTDPSDTRGYSLLKSEEVPQGSTLGVDFIDTLLLYQLTENEKLDKPFEYLNDCFRRNQQQKRITKNKPNAESLHSTFQEIDRLVIGYGVVALQIENFCMNGAFINYITGIVSNVNSYTDFLSQIIQRAILEGTALDLLNAVFPTLLEYCNKHVSHFDLNESVIYNNVLTIFELFVTFKPIAEIFTKIDGFFADYSCKPQDFERKTILGPILSLSPIEAAVAIRNYGDNLLRSKQQTAMIHESLQAEHKVVIDRLFFIVDKLVRGSLNSRTDMISYFAHIANKNHLRRADHPPFKELSSNGFMSNITLLLVRFSQPFLDISYKKIDKIDANYFNNPSLFIDLSGETRLNSDFKEADAFYDKNRKTADSKPNFISDCFFLTLTYLHYGLGGTLSFEEKMGSEIKALKEEIEKVKKIAANHDVFARFITAQLSKMEKALKTTESLRFALQGFFAHRSLQLEVFDFICGASTFLIRVVDPEHEFPFKQIKLPLIPDQIGVENVDNADFLRAHAPVPFKYYPEFVVEGPVNYSLYISKYQTSPIFRNPRLGSFVEFTTMVLRCPELVSNPHLKGKLVQLLSVGAMPLTDNSPGFMMDIFEHDELVNKNLLYALLDFYVIVEKTGSSSQFYDKFNSRYSISIILEELYYKIPSYKNQLIWQSQNNADFFVRFVARMLNDLTFLLDEGLSNLAEVHNIQNELDNRARGAPPTREEEDKELQTRLASASRQAKSSCGLADKSMKLFEIYSKDIPAAFVTPEIVYRLASMLNYNLESLVGPKCGELKVKDPQSYSFNPKDLLKALTTVYINLSEQSEFISAVAKDERSFNRNLFVRAVDILGRKTGLASPEFIEKLLNFANKAEEQRKADEEEDLEYGDVPDEFLDPLMYTIMKDPVILPASKMNIDRSTIKAHLLSDSTDPFNRMPLKLEDVTPNEELRQKILCFKKQKKEEAKHKASE 877ACB59AC29634A 877ACB59AC29634A rpn1_yeast 26S proteasome regulatory subunit RPN1 RPN1 HRD2 NAS1 RPD1 Proteasome non-ATPase subunit 1 HMG-CoA reductase degradation protein 2 YHR027C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVDESDKKQQTIDEQSQISPEKQTPNKKDKKKEEEEQLSEEDAKLKTDLELLVERLKEDDSSLYEASLNALKESIKNSTSSMTAVPKPLKFLRPTYPDLCSIYDKWTDPNLKSSLADVLSILAMTYSENGKHDSLRYRLLSDVSDFEGWGHEYIRHLALEIGEVYNDQVEKDAEDETSSDGSKSDGSAATSGFEFSKEDTLRLCLDIVPYFLKHNGEEDAVDLLLEIESIDKLPQFVDENTFQRVCQYMVACVPLLPPPEDVAFLKTAYSIYLSQNELTDAIALAVRLGEEDMIRSVFDATSDPVMHKQLAYILAAQKTSFEYEGVQDIIGNGKLSEHFLYLAKELNLTGPKVPEDIYKSHLDNSKSVFSSAGLDSAQQNLASSFVNGFLNLGYCNDKLIVDNDNWVYKTKGDGMTSAVASIGSIYQWNLDGLQQLDKYLYVDEPEVKAGALLGIGISASGVHDGEVEPALLLLQDYVTNPDTKISSAAILGLGIAFAGSKNDEVLGLLLPIAASTDLPIETAAMASLALAHVFVGTCNGDITTSIMDNFLERTAIELKTDWVRFLALALGILYMGQGEQVDDVLETISAIEHPMTSAIEVLVGSCAYTGTGDVLLIQDLLHRLTPKNVKGEEDADEEETAEGQTNSISDFLGEQVNEPTKNEEAEIEVDEMEVDAEGEEVEVKAEITEKKNGESLEGEEIKSEEKKGKSSDKDATTDGKNDDEEEEKEAGIVDELAYAVLGIALIALGEDIGKEMSLRHFGHLMHYGNEHIRRMVPLAMGIVSVSDPQMKVFDTLTRFSHDADLEVSMNSIFAMGLCGAGTNNARLAQLLRQLASYYSREQDALFITRLAQGLLHLGKGTMTMDVFNDAHVLNKVTLASILTTAVGLVSPSFMLKHHQLFYMLNAGIRPKFILALNDEGEPIKVNVRVGQAVETVGQAGRPKKITGWITQSTPVLLNHGERAELETDEYISYTSHIEGVVILKKNPDYREEE B77954F4A998BA7C B77954F4A998BA7C rad4_yeast DNA repair protein RAD4 YER162C RAD4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNEDLPKEYFELIRKALNEKEAEKAPLSRRRRVRRKNQPLPDAKKKFKTGLNELPRESVVTVNLDSSDDGVVTVPTDDSVEEIQSSEEDYDSEEFEDVTDGNEVAGVEDISVEIKPSSKRNSDARRTSRNVCSNEERKRRKYFHMLYLVCLMVHGFIRNEWINSKRLSRKLSNLVPEKVFELLHPQKDEELPLRSTRKLLDGLKKCMELWQKHWKITKKYDNVGLYMRTWKEIEMSANNKRKFKTLKRSDFLRAVSKGHGDPDISVQGFVAMLRACNVNARLIMSCQPPDFTNMKIDTSLNGNNAYKDMVKYPIFWCEVWDKFSKKWITVDPVNLKTIEQVRLHSKLAPKGVACCERNMLRYVIAYDRKYGCRDVTRRYAQWMNSKVRKRRITKDDFGEKWFRKVITALHHRKRTKIDDYEDQYFFQRDESEGIPDSVQDLKNHPYYVLEQDIKQTQIVKPGCKECGYLKVHGKVGKVLKVYAKRDIADLKSARQWYMNGRILKTGSRCKKVIKRTVGRPKGEAEEEDERLYSFEDTELYIPPLASASGEITKNTFGNIEVFAPTMIPGNCCLVENPVAIKAARFLGVEFAPAVTSFKFERGSTVKPVLSGIVVAKWLREAIETAIDGIEFIQEDDNRKEHLLGALESWNTLLLKLRIRSKLNSTYGKIAEEEPNVTKEQNIADNHDNTETFMGGGFLPGIANHEARPYSEPSEPEDSLDYVSVDKAEESATDDDVGEDYSDFMKELEMSEESD 788D3B6F580D2BF8 788D3B6F580D2BF8 png1-rad23-2 3 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 6 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C Derivatives of Rad23 containing various functional domains (Fig. 1 A) were tested in the GST pull-down assay for interaction with Png1 (Fig. 1 C). rad23-png1-11 1 5 unspecified role unspecified role prey prey Q02890:p.His218Tyr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H Y Wed May 02 14:32:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his218tyr' Tue Jun 30 18:27:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His218Tyr' 6 unspecified role unspecified role bait bait P32628:p.Leu276Gln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 17:44:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu276gln' Tue Jun 30 18:22:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu276Gln' region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain P32628:p.[Leu183Ala;Leu392Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:30:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu183ala,leu392ala' Tue Jun 30 19:14:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu183Ala;Leu392Ala]' physical association physical association Fig. 1D, 3A,3C, 4B derivatives of Rad23 (Rao and Sastry, 2002) were tested for interaction with Png1 by the two-hybrid assay (Fig. 1 D). rad23-rpn1-6 1 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait P32628:p.Leu276Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 18:46:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu276gln' Tue Jun 30 20:01:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu276Gln' P32628:p.[Leu183Ala;Leu392Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:02:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu183ala,leu392ala' Tue Jun 30 16:50:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu183Ala;Leu392Ala]' physical association physical association Fig.4B, 3C rad23-rad4-14 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait P32628:p.Leu276Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 16:29:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu276gln' Tue Jun 30 19:09:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu276Gln' physical association physical association Fig. 4B rad23-ufd2-5 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait P32628:p.Leu276Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 15:16:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu276gln' Tue Jun 30 18:25:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu276Gln' physical association physical association Fig. 4B, 5A rad23-rad23-1 1 6 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait P32628:p.Leu276Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 19:08:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu276gln' Tue Jun 30 16:17:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu276Gln' physical association physical association Fig. 4B rad23-png1-12 4 5 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role bait bait purified purified P32628:p.Leu276Gln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L Q Wed May 02 15:42:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu276gln' Tue Jun 30 17:59:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu276Gln' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 4C rad23-png1-13 2 7 unspecified role unspecified role prey prey purified purified region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5B