UniProt UniProt http://www.uniprot.org Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Molecular biology of the cell 2006 imex curation Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW., Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW. grant.brown@utoronto.ca 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW., Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW. grant.brown@utoronto.ca Accepted 2017-DEC-11 AT 09:54 GMT AT 09:54 GMT by LIVIAP Molecular biology of the cell 2006 imex curation The reciprocal interaction is already there in roberts-2006-3. I don't agree this should be a pull down - anti-vsv was used to isolate the complex. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Molecular biology of the cell 2006 imex curation Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW., Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW. grant.brown@utoronto.ca 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW., Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW. grant.brown@utoronto.ca Accepted 2017-DEC-11 AT 09:54 GMT AT 09:54 GMT by LIVIAP Molecular biology of the cell 2006 imex curation The reciprocal interaction is already there in roberts-2006-3. I don't agree this should be a pull down - anti-vsv was used to isolate the complex. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Molecular biology of the cell 2006 imex curation Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW., Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW. grant.brown@utoronto.ca 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW., Roberts TM., Kobor MS., Bastin-Shanower SA., Ii M., Horte SA., Gin JW., Emili A., Rine J., Brill SJ., Brown GW. grant.brown@utoronto.ca Accepted 2017-DEC-11 AT 09:54 GMT AT 09:54 GMT by LIVIAP Molecular biology of the cell 2006 imex curation The reciprocal interaction is already there in roberts-2006-3. I don't agree this should be a pull down - anti-vsv was used to isolate the complex. rt107_yeast Regulator of Ty1 transposition protein 107 RTT107 ESC4 Establishes silent chromatin protein 4 YHR154W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDSVKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYHFKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVEILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLEQCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEKPPPTNDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQIHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKYVLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKNVLYQKKNN 767931285BB52580 767931285BB52580 rla1_yeast 60S acidic ribosomal protein P1-alpha RPA1 RPLA1 RPP1A L12EIIA L12eIIA YP1alpha YDL081C Large ribosomal subunit protein P1-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTESALSYAALILADSEIEISSEKLLTLTNAANVPVENIWADIFAKALDGQNLKDLLVNFSAGAAAPAGVAGGVAGGEAGEAEAEKEEEEAKEESDDDMGFGLFD 9A2FE94D28640ACC ssb1_yeast Ribosome-associated molecular chaperone SSB1 YDL229W SSB1 YG101 Cold-inducible protein YG101 Heat shock protein SSB1 Hsp70 chaperone Ssb protein protein yeast Saccharomyces cerevisiae Baker's yeast MAEGVFQGAIGIDLGTTYSCVATYESSVEIIANEQGNRVTPSFVAFTPEERLIGDAAKNQAALNPRNTVFDAKRLIGRRFDDESVQKDMKTWPFKVIDVDGNPVIEVQYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQATKDAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGGVYTVKSTSGNTHLGGQDFDTNLLEHFKAEFKKKTGLDISDDARALRRLRTAAERAKRTLSSVTQTTVEVDSLFDGEDFESSLTRARFEDLNAALFKSTLEPVEQVLKDAKISKSQIDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDETKDLLLLDVAPLSLGVGMQGDMFGIVVPRNTTVPTIKRRTFTTCADNQTTVQFPVYQGERVNCKENTLLGEFDLKNIPMMPAGEPVLEAIFEVDANGILKVTAVEKSTGKSSNITISNAVGRLSSEEIEKMVNQAEEFKAADEAFAKKHEARQRLESYVASIEQTVTDPVLSSKLKRGSKSKIEAALSDALAALQIEDPSADELRKAEVGLKRVVTKAMSSR F16FA7C25A40321A F16FA7C25A40321A rla0_yeast 60S acidic ribosomal protein P0 RPP0 L10E RPA0 RPL10E RPLA0 YLR340W L8300.8 L10e Large ribosomal subunit protein uL10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGGIREKKAEYFAKLREYLEEYKSLFVVGVDNVSSQQMHEVRKELRGRAVVLMGKNTMVRRAIRGFLSDLPDFEKLLPFVKGNVGFVFTNEPLTEIKNVIVSNRVAAPARAGAVAPEDIWVRAVNTGMEPGKTSFFQALGVPTKIARGTIEIVSDVKVVDAGNKVGQSEASLLNLLNISPFTFGLTVVQVYDNGQVFPSSILDITDEELVSHFVSAVSTIASISLAIGYPTLPSVGHTLINNYKDLLAVAIAASYHYPEIEDLVDRIENPEKYAAAAPAATSAASGDAAPAEEAAAEEEEESDDDMGFGLFD 109ECC90F7C8B68E 109ECC90F7C8B68E rs24a_yeast 40S ribosomal protein S24-A RPS24A RPS24EA RP50 Small ribosomal subunit protein eS24-A YER074W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDAVTIRTRKVISNPLLARKQFVVDVLHPNRANVSKDELREKLAEVYKAEKDAVSVFGFRTQFGGGKSVGFGLVYNSVAEAKKFEPTYRLVRYGLAEKVEKASRQQRKQKKNRDKKIFGTGKRLAKKVARRNAD 81B82D361207442A rs20_yeast 40S ribosomal protein S20 RPS20 URP2 YHL015W Small ribosomal subunit protein uS10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDFQKEKVEEQEQQQQQIIKIRITLTSTKVKQLENVSSNIVKNAEQHNLVKKGPVRLPTKVLKISTRKTPNGEGSKTWETYEMRIHKRYIDLEAPVQIVKRITQITIEPGVDVEVVVASN 8B1607EDB63017F1 ipt1_yeast Inositolphosphotransferase 1 IPT1 SYR4 YDR072C D4405 YD8554.05C Mannosyl diphosphorylinositol ceramide synthase protein protein yeast Saccharomyces cerevisiae Baker's yeast MNVIFSLASFVKNMYNASLNQRNLISLPFNFMLNFAPVFIWLSIFKRAGLIPIRLRPDIHSKFAFFADQFLFGDYWHELTVQLPDNTSKLFFWSFISSSAFLLVFLICIPFAIWYYIYYIKHVNYNLLEWFANIFHYPCKRKQRPIQKRFRTIFIPFALPLFTFVILNIDHFFAYQSDANFTKTKDLLAWFSYVILHLTAPILTAVYLYVFQPPGTLKCFSFALGLQNIAGVLTHLLVPMASPWFTHLYGIDDTEHVNYTQEGFAAGLIRVDSHLGTHLNTKGFHMSPIVFGAVPSLHSAIAFQCFLFLVSRSTSLKHRFSNAGGFTMHNNDSSTFKLSEEDSEDEGDNSIPPTIGPNDLEMEPLGTVEPVDISNERSSSPSSSFTVSSNERSTGGGDGSIINSNGNKKPLQFVHLYDEDTNFTNKWIFKIVNDGFIPKFWAILYIILQWWATMYLDHHYRFDLFVGVLYAMTSFIIINWFVLQPKVLKKWIHIRLGDKVDTRNEARTFGMRVFCGTKMEWFFDPLA 0792FE736A560BAE 0792FE736A560BAE ef1a_yeast Elongation factor 1-alpha TEF1 YPR080W P9513.7 TEF2 YBR118W YBR0913 Translation elongation factor 1A Eukaryotic elongation factor 1A protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFLKSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTEKAAKVTKAAQKAAKK 411C66D830716576 411C66D830716576 slx4_yeast Structure-specific endonuclease subunit SLX4 SLX4 L3140 YLR135W Synthetic lethal of unknown function protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK 52C0FD889C8C5835 52C0FD889C8C5835 rla2_yeast 60S acidic ribosomal protein P2-alpha RPP2A L12EIB RPA2 RPL44 RPLA2 L12eIB YP2alpha L44 YOL039W Large ribosomal subunit protein P2-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MKYLAAYLLLNAAGNTPDATKIKAILESVGIEIEDEKVSSVLSALEGKSVDELITEGNEKLAAVPAAGPASAGGAAAASGDAAAEEEKEEEAAEESDDDMGFGLFD 22275AFA35E1A32E pmg1_yeast Phosphoglycerate mutase 1 GPM1 GPM YKL152C YKL607 Phosphoglyceromutase 1 MPGM 1 BPG-dependent PGAM 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPKLVLVRHGQSEWNEKNLFTGWVDVKLSAKGQQEAARAGELLKEKKVYPDVLYTSKLSRAIQTANIALEKADRLWIPVNRSWRLNERHYGDLQGKDKAETLKKFGEEKFNTYRRSFDVPPPPIDASSPFSQKGDERYKYVDPNVLPETESLALVIDRLLPYWQDVIAKDLLSGKTVMIAAHGNSLRGLVKHLEGISDADIAKLNIPTGIPLVFELDENLKPSKPSYYLDPEAAAAGAAAVANQGKK 45E1A9CCDBDC104D 45E1A9CCDBDC104D hsp71_yeast Heat shock protein SSA1 YAL005C SSA1 Heat shock protein YG100 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKAVGIDLGTTYSCVAHFANDRVDIIANDQGNRTTPSFVAFTDTERLIGDAAKNQAAMNPSNTVFDAKRLIGRNFNDPEVQADMKHFPFKLIDVDGKPQIQVEFKGETKNFTPEQISSMVLGKMKETAESYLGAKVNDAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKGKEEHVLIFDLGGGTFDVSLLSIEDGIFEVKATAGDTHLGGEDFDNRLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQTSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLRDAKLDKSQVDEIVLVGGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLDVAPLSLGIETAGGVMTKLIPRNSTIPTKKSEIFSTYADNQPGVLIQVFEGERAKTKDNNLLGKFELSGIPPAPRGVPQIEVTFDVDSNGILNVSAVEKGTGKSNKITITNDKGRLSKEDIEKMVAEAEKFKEEDEKESQRIASKNQLESIAYSLKNTISEAGDKLEQADKDTVTKKAEETISWLDSNTTASKEEFDDKLKELQDIANPIMSKLYQAGGAPGGAAGGAPGGFPGGAPPAPEAEGPTVEEVD 249D8A3BC42527CA 249D8A3BC42527CA slx1_yeast Structure-specific endonuclease subunit SLX1 YBR228W YBR1525 SLX1 Synthetic lethal of unknown function protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQKIQQHQFPDFYCCYLLQSINKRQSFYVGSTPNPVRRLRQHNGKLAVGGAYRTKRDGSRPWEMIMIVRGFPSKIAALQFEHAWQHGYQTHYIAEKDRVVKHKAGGRTLHHKVALMKLLLKHEFFQRMNLIVEVFNIKAWEVWKQDKFFIERDRFPINIQINENALEEPKEKTVDVLMDHSDENLKVVEAVYTKVIENERNIFETFEKKLTTGVVRCEICEKEIDYTSEEQNLKPFVALCNNKDCGCVNHLKCLHRYFLDDEQLMVGRRNLIPRGGKCPKCDMFCDWTTLVKFSTRMKLAHGK 4C676E877FDF7A7A 4C676E877FDF7A7A g3p3_yeast Glyceraldehyde-3-phosphate dehydrogenase 3 TDH3 GPD3 YGR192C G7576 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVRVAINGFGRIGRLVMRIALSRPNVEVVALNDPFITNDYAAYMFKYDSTHGRYAGEVSHDDKHIIVDGKKIATYQERDPANLPWGSSNVDIAIDSTGVFKELDTAQKHIDAGAKKVVITAPSSTAPMFVMGVNEEKYTSDLKIVSNASCTTNCLAPLAKVINDAFGIEEGLMTTVHSLTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVLPELQGKLTGMAFRVPTVDVSVVDLTVKLNKETTYDEIKKVVKAAAEGKLKGVLGYTEDAVVSSDFLGDSHSSIFDASAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVAKA 6CFFFEE7061BC36F 6CFFFEE7061BC36F rtt107-gpm1-1 1 8 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey living cell living cell naturally occurring naturally occurring endogenous endogenous 17 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association Table 2 slx4-rtt107-1 3 4 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, 2A, 2B Rtt107-VSV coprecipitated with Slx4-TAP and vice versa (Figure 1A) Interaction observed in SLX1-/- cells and is unaffected by MMS induced DNA damage. rtt107-slx4-2 3 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1B rtt107-slx4-3 2 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A Rtt107-VSV coprecipitated with Slx4-TAP and vice versa (Figure 1A) rtt107-slx1-1 3 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1B rtt107-slx4-5 3 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2E