DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer. E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer. Nature structural & molecular biology 2005 imex curation Eletr ZM., Huang DT., Duda DM., Schulman BA., Kuhlman B. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Eletr ZM., Huang DT., Duda DM., Schulman BA., Kuhlman B. DIP original experiment: DIP-255134X Nature structural & molecular biology 2005 IMEx E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer. p68036-1 Ubiquitin-conjugating enzyme E2 L3 E2 ubiquitin-conjugating enzyme L3 L-UBC UbcH7 Ubiquitin carrier protein L3 Ubiquitin-conjugating enzyme E2-F1 Ubiquitin-protein ligase L3 UBE2L3 UBCE7 UBCH7 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD DIP protein P68036-1 original sequence version: 102 DIP protein P68036 original sequence version: 98 DIP protein P68036 original sequence version: 48 DIP protein P68036 original sequence version: 103 DIP protein P68036 original sequence version: 77 DIP protein P68036-1 original sequence version: 109 q05086-2 Ubiquitin-protein ligase E3A E6AP E6AP ubiquitin-protein ligase EPVE6AP HPVE6A Human papillomavirus E6-associated protein Oncogenic protein-associated protein E6-AP Renal carcinoma antigen NY-REN-54 UBE3A HECT-type ubiquitin transferase E3A protein protein human Homo sapiens Human MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML Variant in position: 212:D->V (in AS, unknown pathological significance). Variant in position: 237:L->Q (in AS, unknown pathological significance). Variant in position: 263:L->W (in AS, unknown pathological significance). Variant in position: 458:P->L (in AS, unknown pathological significance). Variant in position: 545:G->R (in AS, unknown pathological significance). Variant in position: 584:E->Q (in AS, unknown pathological significance). Variant in position: 656:T->I (in AS, unknown pathological significance). Variant in position: 690:F->C (in AS, unknown pathological significance). Variant in position: 827:P->L (in AS, unknown pathological significance). Variant in position: 106:T->K (in AS, unknown pathological significance). Variant in position: 237:L->H (in AS, unknown pathological significance). Variant in position: 435:L->P (in AS, unknown pathological significance). Variant in position: 477:R->P (in AS, unknown pathological significance). Variant in position: 566:M->K (in AS, unknown pathological significance). Variant in position: 117:C->R (common polymorphism, may be associated with AS). Variant in position: 270:N->T (common polymorphism, may be associated with AS). Variant in position: 762:V->I (common polymorphism, may be associated with AS). Variant in position: 133:V->G (common polymorphism, may be associated with AS). Variant in position: 335:S->T (common polymorphism, may be associated with AS). Variant in position: 478:M->I (common polymorphism, may be associated with AS). Variant in position: 588:Q->E (common polymorphism, may be associated with AS). Variant in position: 588:Q->P (common polymorphism, may be associated with AS). Variant in position: 673:L->R (common polymorphism, may be associated with AS). DIP protein Q05086-2 original sequence version: 140 DIP protein Q05086-2 original sequence version: 118 3C061DA8D216055A ube2l3-ube3a-2 1 3 unspecified role unspecified role unspecified role unspecified role Q05086-2:p.Cys820Ala C820A mutation mutation certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C820A'. Wed May 02 20:26:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys820ala' Tue Jun 30 17:42:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys820Ala' HCET domain Region 492-852 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 2 unspecified role unspecified role unspecified role unspecified role P68036-1:p.Phe63Asp F63D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F D This feature has been corrected as a result of our quality control procedures. The original label was 'F63D'. Wed May 02 14:41:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe63asp' Tue Jun 30 17:20:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe63Asp' BODIPY-label BODIPY-label bodipy label bodipy label n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction