DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255146X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions mesau Mesocricetus auratus (Golden hamster) Golden hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255142X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions mesau Mesocricetus auratus (Golden hamster) Golden hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255145X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255147X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255141X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions mesau Mesocricetus auratus (Golden hamster) Golden hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255143X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. Nature structural & molecular biology 2005 imex curation Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. Only protein-protein interactions mesau Mesocricetus auratus (Golden hamster) Golden hamster fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Brown RJ., Adams JJ., Pelekanos RA., Wan Y., McKinstry WJ., Palethorpe K., Seeber RM., Monks TA., Eidne KA., Parker MW., Waters MJ. DIP original experiment: DIP-255144X Nature structural & molecular biology 2005 IMEx Model for growth hormone receptor activation based on subunit rotation within a receptor dimer. lepr_human Leptin receptor OB receptor HuB219 LEPR DB OBR protein protein human Homo sapiens Human MICQKFCVVLLHWEFIYVITAFNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSDAGLYVIVPVIISSSILLLGTLLISHQRMKKLFWEDVPNPKNCSWAQGLNFQKPETFEHLFIKHTASVTCGPLLLEPETISEDISVDTSWKNKDEMMPTTVVSLLSTTDLEKGSVCISDQFNSVNFSEAEGTEVTYEDESQRQPFVKYATLISNSKPSETGEEQGLINSSVTKCFSSKNSPLKDSFSNSSWEIEAQAFFILSDQHPNIISPHLTFSEGLDELLKLEGNFPEENNDKKSIYYLGVTSIKKRESGVLLTDKSRVSCPFPAPCLFTDIRVLQDSCSHFVENNINLGTSSKKTFASYMPQFQTCSTQTHKIMENKMCDLTV DIP protein P48357 original sequence version: 151 DIP protein P48357 original sequence version: 96 DIP protein P48357 original sequence version: 135 CAA03BEAF2602D0A ghr_human Growth hormone receptor Somatotropin receptor GHR protein protein human Homo sapiens Human MDLWQLLLTLALAGSSDAFSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNGGTVDEKCFSVDEIVQPDPPIALNWTLLNVSLTGIHADIQVRWEAPRNADIQKGWMVLEYELQYKEVNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQFTCEEDFYFPWLLIIIFGIFGLTVMLFVFLFSKQQRIKMLILPPVPVPKIKGIDPDLLKEGKLEEVNTILAIHDSYKPEFHSDDSWVEFIELDIDEPDEKTEESDTDRLLSSDHEKSHSNLGVKDGDSGRTSCCEPDILETDFNANDIHEGTSEVAQPQRLKGEADLLCLDQKNQNNSPYHDACPATQQPSVIQAEKNKPQPLPTEGAESTHQAAHIQLSNPSSLSNIDFYAQVSDITPAGSVVLSPGQKNKAGMSQCDMHPEMVSLCQENFLMDNAYFCEADAKKCIPVAPHIKVESHIQPSLNQEDIYITTESLTTAAGRPGTGEHVPGSEMPVPDYTSIHIVQSPQGLILNATALPLPDKEFLSSCGYVSTDQLNKIMP DIP protein P10912 original sequence version: 166 EAF77EADE4787822 epor_human Erythropoietin receptor EPOR protein protein human Homo sapiens Human MDHLGASLWPQVGSLCLLLAGAAWAPPPNLPDPKFESKAALLAARGPEELLCFTERLEDLVCFWEEAASAGVGPGNYSFSYQLEDEPWKLCRLHQAPTARGAVRFWCSLPTADTSSFVPLELRVTAASGAPRYHRVIHINEVVLLDAPVGLVARLADESGHVVLRWLPPPETPMTSHIRYEVDVSAGNGAGSVQRVEILEGRTECVLSNLRGRTRYTFAVRARMAEPSFGGFWSAWSEPVSLLTPSDLDPLILTLSLILVVILVLLTVLALLSHRRALKQKIWPGIPSPESEFEGLFTTHKGNFQLWLYQNDGCLWWSPCTPFTEDPPASLEVLSERCWGTMQAVEPGTDDEGPLLEPVGSEHAQDTYLVLDKWLLPRNPPSEDLPGPGGSVDIVAMDEGSEASSCSSALASKPSPEGASAASFEYTILDPSSQLLRPWTLCPELPPTPPHLKYLYLVVSDSGISTDYSSGDSQGAQGGLSDGPYSNPYENSLIPAAEPLPPSYVACS DIP protein P19235 original sequence version: 168 DIP protein P19235 original sequence version: 186 F9F326E162E9512A ghr-ghr-1 5 9 unspecified role unspecified role prey prey Region 1-322 Region 1-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 9 unspecified role unspecified role bait bait Region 1-322 Region 1-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster physical association physical association ghr-1 4 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 241-322 Region 241-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 9 unspecified role unspecified role fluorescence donor fluorescence donor Region 241-322 Region 241-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey proximity proximity close-contact colocalization neighbourhood interaction ghr-2 6 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 241-275 Region 241-275 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster 9 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 241-275 Region 241-275 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain mesau Mesocricetus auratus (Golden hamster) Golden hamster proximity proximity close-contact colocalization neighbourhood interaction epor-1 3 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster 10 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster proximity proximity close-contact colocalization neighbourhood interaction ghr-3 2 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-322 Region 1-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster 9 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-322 Region 1-322 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster proximity proximity close-contact colocalization neighbourhood interaction lepr-1 1 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 8 unspecified role unspecified role fluorescence donor fluorescence donor Rluc-tag Rluc-tag renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey proximity proximity close-contact colocalization neighbourhood interaction lepr-2 7 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster 8 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau Mesocricetus auratus (Golden hamster) Golden hamster proximity proximity close-contact colocalization neighbourhood interaction