DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254772X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254776X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254775X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254770X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254773X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254771X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nature structural & molecular biology 2005 imex curation Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Collins BM., Skinner CF., Watson PJ., Seaman MN., Owen DJ. DIP original experiment: DIP-254774X Nature structural & molecular biology 2005 IMEx Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. vps17_yeast Vacuolar protein sorting-associated protein 17 VPS17 PEP21 YOR132W O3314 YOR3314W Carboxypeptidase Y-deficient protein 21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSAVPYDPYDDLDNNPFAEPQEEDSEPAATTTDGSSSMSEERVGTEQTAASVQDNGTANNIQNGLGEEGNATRSKTSNEHNENQQPSQPSERVILPERSDEKKKYTLLAKVTGLERFGSATGKKENPTIIFDCSTNLPTFRKQQYKNVKKSYEEFHQLFKYLNVAIQESFVPTLPSAYTTFGINSEEDRMKVTRNFQLWFNRLSQDPLIIRNEEVAFFIESDFNTYTPINKSKSLASGLKRKTLKQLAPPYDEITELAEFRPLVKSIYVVSQSLQEKLLRVSRNRKMMVQEENAFGQDFVNLDEHNKLYRRYGKILTAVGDIDSIIATMDMATLYDGLEWIVRDAYAVKEALTNRHFIMRNLVQAQQNSKAKQEQARRFRSRRDINPMKIDEALRQLKAAAKNEQVLTLKLQRITSNMIIERKQWISWYEEWIRSSIKEFTLRKIEYERKKLTLLERVRSDIRKADENGGLSRLGRHAVSNNNSDTSQTLKGDSWTGESNRKSQIPINKIAHTEFDDELFTEDDGYNSQDSDTTSLNARHAASLLGMSTK C709968468144A50 C709968468144A50 pep8_yeast Carboxypeptidase Y-deficient protein 8 PEP8 GRD6 VPS26 VPT4 J1152 YJL053W Vacuolar protein-targeting protein 4 Vacuolar protein sorting-associated protein 26 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIFFKPPIDIEILFDNEESRKHVDIATRSSNSSYKSMKESLPVYEDGESLGGIVTLRVRDSKKVDHLGIKVSVIGSIDMLKSHGSGNSSSKKVTSSTSSSSSNGSVDVRKNSVDQFLCQSYDLCPAGELQHSQSFPFLFRDLSKRYESYKGKNVDVAYYVKVTVMRKSTDISKIKRFWVYLYNSVTTAPNTLSANETKATTNDIAGGNYAADNASDNTQTKSTQGEAADVNQVLPISHSNNEPKPVRLDIGIENCLHIEFEYAKSQYSLKEVIVGRIYFLLTRLRIKHMELSLITRESSGLQTSNVMTDSTAIRYEIMDGSSVKGETIPIRLFLSGYDLTPNMSCNYFNVKNYLSLVIIDEDGRRYFKQSEITLYRTR 40D15594E70A9B58 40D15594E70A9B58 vps35_human Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 VPS35 MEM3 TCCCTA00141 protein protein human Homo sapiens Human MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL DIP protein Q96QK1 original sequence version: 118 DIP protein Q96QK1 original sequence version: 44 DIP protein Q96QK1 original sequence version: 117 DIP protein Q96QK1 original sequence version: 127 28D2DD1C6B920A0A vps35_mouse Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 Vps35 Mem3 protein protein mouse Mus musculus Mouse MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRSRRESPESEGPIYEGLIL DIP protein Q9EQH3 original sequence version: 93 DIP protein Q9EQH3 original sequence version: 57 2ABD338111D641CC q9qz88-1 Vacuolar protein sorting-associated protein 29 Vesicle protein sorting 29 Vps29 protein protein mouse Mus musculus Mouse MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKS DIP protein Q9QZ88-1 original sequence version: 103 vp26a_human Vacuolar protein sorting-associated protein 26A VPS26A VPS26 Vesicle protein sorting 26A protein protein human Homo sapiens Human MSFLGGFFGPICEIDIVLNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM DIP protein O75436 original sequence version: 115 DIP protein O75436 original sequence version: 53 DIP protein O75436 original sequence version: 113 DIP protein O75436-1 original sequence version: 113 DIP protein O75436 original sequence version: 123 BD3B2EAA6CFCBFA9 vps35_yeast Vacuolar protein sorting-associated protein 35 VPS35 Vacuolar protein-targeting protein 7 VPT7 J0580 YJL154C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAYADSPENAIAVIKQRTALMNRCLSQHKLMESLQHTSIMLTELRNPNLSPKKYYELYVIIFDSLTNLSTYLIENHPQNHHLADLYELVQYTGNVVPRLYLMITVGTSYLTFNEAPKKEILKDMIEMCRGVQNPIRGLFLRYYLSQRTKELLPEDDPSFNSQFIMNNFIEMNKLWVRLQHQGPLRERETRTRERKELQILVGSQLVRLSQIIDDNFQMYKQDILPTILEQVIQCRDLVSQEYLLDVICQVFADEFHLKTLDTLLQTTLHLNPDVSINKIVLTLVDRLNDYVTRQLEDDPNATSTNAYLDMDVFGTFWDYLTVLNHERPDLSLQQFIPLVESVIVLSLKWYPNNFDNLNKLFELVLQKTKDYGQKNISLESEHLFLVLLSFQNSKLQLTSSTTAPPNSPVTSKKHFIFQLISQCQAYKNILALQSISLQKKVVNEIIDILMDREVEEMADNDSESKLHPPGHSAYLVIEDKLQVQRLLSICEPLIISRSGPPANVASSDTNVDEVFFNRHDEEESWILDPIQEKLAHLIHWIMNTTSRKQTMKNKIQFSLEAQLEILLLIKSSFIKGGINVKYTFPAIITNFWKLMRKCRMIQEYLLKKRPDNKTLLSHYSNLLKQMFKFVSRCINDIFNSCNNSCTDLILKLNLQCAILADQLQLNEISYDFFSQAFTIFEESLSDSKTQLQALIYIAQSLQKTRSLYKEAYYDSLIVRCTLHGSKLLKKQDQCRAVYLCSHLWWATEISNIGEEEGITDNFYRDGKRVLECLQRSLRVADSIMDNEQSCELMVEILNRCLYYFIHGDESETHISIKYINGLIELIKTNLKSLKLEDNSASMITNSISDLHITGENNVKASSNADDGSVITDKESNVAIGSDGTYIQLNTLNGSSTLIRGVVATASGSKLLHQLKYIPIHHFRRTCEYIESQREVDDRFKVIYV DE1BC57B9DFB606E DE1BC57B9DFB606E vps29_yeast Vacuolar protein sorting-associated protein 29 VPS29 PEP11 Vesicle protein sorting 29 Carboxypeptidase Y-deficient protein 11 VTP6 YHR012W protein protein yeast Saccharomyces cerevisiae Baker's yeast MLLLALSDAHIPDRATDLPVKFKKLLSVPDKISQVALLGNSTKSYDFLKFVNQISNNITIVRGEFDNGHLPSTKKDKASDNSRPMEEIPMNSIIRQGALKIGCCSGYTVVPKNDPLSLLALARQLDVDILLWGGTHNVEAYTLEGKFFVNPGSCTGAFNTDWPIVFDVEDSDEAVTSEVDKPTKENQSEDDDAKGGSTGKEQPGSYTPKEGTAGERENENESNVKPENQFKEDEVDMSDSDINGSNSPSFCLLDIQGNTCTLYIYLYVNGEVKVDKVVYEKE D190DBA2377E0653 D190DBA2377E0653 p40336-1 Vacuolar protein sorting-associated protein 26A H<beta>58 protein Vesicle protein sorting 26A Vps26a Vps26 protein protein mouse Mus musculus Mouse MSFLGGFFGPICEIDVALNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPDSQASAEQPEM DIP protein P40336-1 original sequence version: 105 vps26p-vps17p-1 4 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 14 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 15 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association vps35-vps26a-6 3 16 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-390 Region 1-390 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction vps29-vps35-9 5 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction vps35-vps26a-7 7 16 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey ecolx Escherichia coli association association vps29-vps26a-2 1 10 unspecified role unspecified role prey prey human Homo sapiens Human 13 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9QZ88-1:p.Val90Asp V90D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V90D'. Wed May 02 15:33:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val90asp' Tue Jun 30 17:51:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val90Asp' human Homo sapiens Human association association vps35-vps29-9 2 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey Q9QZ88-1:p.Ile91Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I S Wed May 02 18:18:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile91ser' Tue Jun 30 17:56:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile91Ser' Q9QZ88-1:p.Val90Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V D Wed May 02 17:09:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val90asp' Tue Jun 30 19:09:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val90Asp' Q9QZ88-1:p.Asn39Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N D Wed May 02 16:30:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn39asp' Tue Jun 30 18:29:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn39Asp' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction vps29-vps26a-3 6 10 unspecified role unspecified role prey prey human Homo sapiens Human 13 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait Q9QZ88-1:p.Asn39Asp N39D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N D This feature has been corrected as a result of our quality control procedures. The original label was 'N39D'. Wed May 02 19:09:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn39asp' Tue Jun 30 16:53:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn39Asp' Q9QZ88-1:p.Val90Asp V90D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V90D'. Wed May 02 18:04:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val90asp' Tue Jun 30 16:33:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val90Asp' Q9QZ88-1:p.Ile91Ser I91S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I S This feature has been corrected as a result of our quality control procedures. The original label was 'I91S'. Wed May 02 15:18:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile91ser' Tue Jun 30 18:51:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile91Ser' GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human association association