UniProt UniProt http://www.uniprot.org Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. The EMBO journal 2005 imex curation Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr Accepted 2017-MAR-08 AT 13:39 GMT AT 13:39 GMT by ORCHARD The EMBO journal 2005 IMEx imex curation Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. The EMBO journal 2005 imex curation Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr Accepted 2017-MAR-08 AT 13:39 GMT AT 13:39 GMT by ORCHARD The EMBO journal 2005 imex curation Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. The EMBO journal 2005 imex curation Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions rat-thyroid rat thyroid gland Rat thyroid_gland confocal microscopy confocal microscopy immunostaining immunostaining Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr Accepted 2017-MAR-08 AT 13:39 GMT AT 13:39 GMT by ORCHARD The EMBO journal 2005 imex curation Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. The EMBO journal 2005 imex curation Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr Accepted 2017-MAR-08 AT 13:39 GMT AT 13:39 GMT by ORCHARD The EMBO journal 2005 IMEx imex curation Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. The EMBO journal 2005 imex curation Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr Accepted 2017-MAR-08 AT 13:39 GMT AT 13:39 GMT by ORCHARD The EMBO journal 2005 imex curation Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. The EMBO journal 2005 imex curation Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line confocal microscopy confocal microscopy immunostaining immunostaining Lahuna O., Quellari M., Achard C., Nola S., Méduri G., Navarro C., Vitale N., Borg JP., Misrahi M. Binding sites for hScrib either according Audebert et al 2004 (PMID 15182672) or UniProt micheline.misrahi@bct.ap-hop-paris.fr Accepted 2017-MAR-08 AT 13:39 GMT AT 13:39 GMT by ORCHARD The EMBO journal 2005 imex curation tshr_rat Thyrotropin receptor Tshr Thyroid-stimulating hormone receptor protein protein rat Rattus norvegicus (Rat) Rat MRPGSLLQLTLLLALPRSLWGRGCTSPPCECHQEDDFRVTCKELHQIPSLPPSTQTLKLIETHLKTIPSLAFSSLPNISRIYLSIDATLQRLEPHSFYNLSKMTHIEIRNTRSLTYIDPDALTELPLLKFLGIFNTGLRIFPDLTKIYSTDVFFILEITDNPYMTSVPENAFQGLCNETLTLKLYNNGFTSIQGHAFNGTKLDAVYLNKNKYLTAIDKDAFGGVYSGPTLLDVSSTSVTALPSKGLEHLKELIAKNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSIRNLRQRKSVNVMRGPVYQEYEEGLGDNHVGYKQNSKFQEGPSNSHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTVCGDNEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSPMALLGNVFVLFVLLTSHYKLTVPRFLMCNLAFADFCMGVYLLLIASVDLYTHTEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHAYTIMAGGWVSCFLLALLPMVGISSYAKVSICLPMDTDTPLALAYIALVLLLNVVAFVIVCSCYVKIYITVRNPQYNPRDKDTKIAKRMAVLIFTDFMCMAPISFYALSALMNKPLITVTNSGVLLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGLCKHQAQAYQAQRVCPNNNTGIQIQKIPQDTRQSLPNVQDTYEPLGSSHLTPKLQGRISEEYTQTAL 79FC45CC9D26167B f1pky2_canlf SCRIB protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPEGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPSSLSFLVKLEQLDLGGNELEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELGGLLLLTDLLLSQNLLQRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLTALPRSLGKLTKLTNLNADRNRLEVLPPEIGGCAALSVLSLRDNRLATLPAELAHTAELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDAQTGEKVLTCYLLPQQPPPSLEESGLQSSPSESWSDAPPSRVSVIQFLEVPMCSDDAEGAAAEKRGLQRRATPHPSELKVMKRGVEERRGEAYSWKPESRLPSPLEEDKRLSTESGLSEDSQPSTGTASQGEPEGSLADTQGLSQQEAAPNAQEEAVEEETYEEPTVRFAEDTLLLPPAGEDGESEEGQLEAPWPLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGAQPDGEGPAGAGGWHNGPHTPWAPRAEEEDEEDEEEDEEEEEVAVAEEDKEEAVVSAPSIKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAAQAGVRVGDKLLEVNGVALHGAEHHQAVEALRGAGTTVQMRLWRERMVEPENAVTVTPLRPEDDYSPRERRGAGLRLPLLQPEAAGPLRQRHVACLVRSEKGLGFSIAGGKGSTPYRAGDGGIFISRIAEGGAAHRAGTLQVGDRVLSINGVDMTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSPPPPVTAPSTVVTASPGESGPLRLAPSLLAATLEGPYPVEEICLPRAGGPLGLSIVGGSDHSSHPFGIQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDIREATHQEAVSALLRPCLELVLLVRRDPPPPGMRELCIQKAPGEKLGISVRGGAKGHAGNPCDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFDTSTVAPAEVSPGVIANPFAAGVGRRNSLESVSSIDRELSPEGCGKEKEPPGPTPQWGLEAMVPPGTTGGKMAEGPHSSSCQQPPSPPSPDTLPTNVKQAYRTFAAVPGPHPLQDTPAQPPTPGPMASPEQLSFRERQKYFELEVRMPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQLMREAEDGALSLDLDGEAPDDEEPEEPPPWAGPAAGLSPSSPQPLGGGAPVRTAKAERRHQERLRVQSPELSTPLPDRALSPAERRALEAEKRALWRAARMKSLEQDALRAQMVLSKSQEGRSRRGPLERLAEAPSPAPTPSPTPVEDLGLQTSTSPGRLALSGRKFDYRVFAALPSSRPVCELQSPDFAEELRSLEPSPSPGLQEEDGEVAMVLLGRPSPGTVGPEEVTLCSSRRPVRPGRRGLGPVPS 388CE7677CD92B4C d3zws0_rat Scrib RGD1565055_predicted rCG_60094 protein protein rat Rattus norvegicus (Rat) Rat MLKCIPLWRCNRHVESVDKRHCSLQVVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPVELGGLALLTDLLLSQNLLQRLPEGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLTALPRSLGKLTKLTNLNVDRNHLEVLPPEIGGCVALSVLSLRDNRLAVLPPELAHTAELHVLDVAGNRLRSLPFALTHLNLKALWLAENQAQPMLRFQTEDDAQTGEKVLTCYLLPQQPLPSLEDPGQQSSPSESCSDAPLSRVSVIQFEDPLEGEEDAEEAAAEKRGLQRRATPHPSELKVMKRGIEERRNEAFVCKPDPSPPSPSEEERRLSAESALSGNSVPSASTASDGEPETLPAEVQGLGQQEAMPGQEEYPEEDYNEPTVHFAEDTLIPREDGEGEEGQPEAPWPLPSGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGVQTDREGPTAGWHNGPHTPWAPRAHEEEEEEENRDEEEGEASMEEDDKEEAVASAPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAAHAGVRVGDKLLEVNGVALQDAEHHEAVEALRGAGAAVQMRVWRERMVEPENAVTITPLRPEDDYSPREWRGGGLRLPLLQPETPVPLRQRHAACLVRSEKGLGFSIAGGKGSTPYRAGDAGIFISRIAEGGAAHRAGTLQVGDRVLSINGVDMTEARHDHAVSLLTAASTTISLLLERETGGAYPPSPPPHSSSTPTATVAATMTTAIPGEPLLPRLSPSLLATALEGPYPVEEICLPRAGGPLGLSIVGGSDHSSHPFGVQDPGVFISKVLPRGLAARCGLRVGDRILAVNGQDVREATHQEAVSALLRPCLELCLLVRRDPPPPGMRELCIQKAPGEKLGISIRGGAKGHAGNPCDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHAEAVQLLRSVGDTLTVLVCDGFDTSTTTALEVSPGVIANPFAAGLGHRNSLESISSIDRELSPEGPGKEKELASQTLPWEPESAETTGRNQEPLKLDYRVLAAVPSAGSLQRGPCATTGGKMTEAPSSPGSQQTKPGVIQPLAQAWPRDSPAPRGRDGPCSPPSPDELPANVKQAYRAFAAVPTVHPPENSATQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQMLREEAVTSGPDTGLASDRESLDDEQEAEQPWAGPSHAGGLSPSSPTPLGGSAPVRTAKAERRHQERLRMQSPELPAPERALSPAERRALEAEKRALWRAARMKSLEQDALRAQMVLSKSQEGRGKRGPLERLAEAPSPAPTPSPTPLEDFGLQTSTSPGRLPLSGKKFDYRAFAALPSSRPVYDIQSPDFVEELRTLDPSPSPGPQEEDGEVALVLLGRPSPGTVGPEDMTLCSSRRAMRPGRRGLGPVPS 2CDC0E50B9473F7B tshr_human Thyrotropin receptor TSHR LGR3 Thyroid-stimulating hormone receptor protein protein human Homo sapiens Human MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLLILLTSHYKLNVPRFLMCNLAFADFCMGMYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHACAIMVGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIVFVLTLNIVAFVIVCCCYVKIYITVRNPQYNPGDKDTKIAKRMAVLIFTDFICMAPISFYALSAILNKPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYRGQRVPPKNSTDIQVQKVTHDMRQGLHNMEDVYELIENSHLTPKKQGQISEEYMQTVL D2EE9CEBFD64A65F scrib_human Protein scribble homolog SCRIB CRIB1 KIAA0147 LAP4 SCRB1 VARTUL Protein LAP4 protein protein human Homo sapiens Human MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQPPPSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPSAEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEVEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERRGGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEGGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRGLQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSPTSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTSTSPGRLSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS DIP protein Q14160 original sequence version: 104 DIP protein Q14160 original sequence version: 139 D71E024FBC4F72D1 tshr-scrib-1 1 11 unspecified role unspecified role prey prey pdz domain 1 sufficient to bind sufficient binding region sufficient to bind range range range range pdz domain 3 sufficient to bind sufficient binding region sufficient to bind range range range range 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2c and d tshr-scrib-2 4 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey cell lysate cell lysate human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 2e tshr-scrib-5 2 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 3A TSH tshr-scrib-6 5 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3C tshr-scrib-7 3 9 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 4B scrib-tshr-1 6 8 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection 10 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization 4A