UniProt UniProt http://www.uniprot.org Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. The Journal of biological chemistry 2005 imex curation Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Only protein-protein interactions chlae-cos Chlorocebus aethiops cos cos confocal microscopy confocal microscopy immunostaining immunostaining Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Accepted 2017-FEB-13 AT 09:09 GMT AT 09:09 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. The Journal of biological chemistry 2005 imex curation Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Accepted 2017-FEB-13 AT 09:09 GMT AT 09:09 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. The Journal of biological chemistry 2005 imex curation Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Accepted 2017-FEB-13 AT 09:09 GMT AT 09:09 GMT by ORCHARD dog thyroid cDNA The Journal of biological chemistry 2005 imex curation Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. Identification of a novel partner of duox: EFP1, a thioredoxin-related protein. The Journal of biological chemistry 2005 imex curation Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Only protein-protein interactions chlae-cos Chlorocebus aethiops cos cos anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont JE., Miot F. CoIP experiments with human thyrocytes using anti-Duox for immunoprecipitation omitted as the antibody recognizes DUOX1 and DUOX2 proteins (here as baits, fig 6D) dawang@ulb.ac.be Accepted 2017-FEB-13 AT 09:09 GMT AT 09:09 GMT by ORCHARD The Journal of biological chemistry 2005 imex curation duox2_human Dual oxidase 2 DUOX2 LNOX2 THOX2 Large NOX 2 Long NOX 2 NADH/NADPH thyroid oxidase p138-tox NADPH oxidase/peroxidase DUOX2 NADPH thyroid oxidase 2 Thyroid oxidase 2 p138 thyroid oxidase protein protein human Homo sapiens Human MLRARPEALMLLGALLTGSLGPSGNQDALSLPWEVQRYDGWFNNLRHHERGAVGCRLQRRVPANYADGVYQALEEPQLPNPRRLSNAATRGIAGLPSLHNRTVLGVFFGYHVLSDVVSVETPGCPAEFLNIRIPPGDPVFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDLANQVTGWLDGSAIYGSSHSWSDALRSFSGGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISPEFVVASEQFFSTMVPPGVYMRNASCHFRKVLNKGFQSSQALRVCNNYWIRENPNLNSTQEVNELLLGMASQISELEDNIVVEDLRDYWPGPGKFSRTDYVASSIQRGRDMGLPSYSQALLAFGLDIPRNWSDLNPNVDPQVLEATAALYNQDLSQLELLLGGLLESHGDPGPLFSAIVLDQFVRLRDGDRYWFENTRNGLFSKKEIEDIRNTTLRDVLVAVINIDPSALQPNVFVWHKGAPCPQPKQLTTDGLPQCAPLTVLDFFEGSSPGFAITIIALCCLPLVSLLLSGVVAYFRGREHKKLQKKLKESVKKEAAKDGVPAMEWPGPKERSSPIIIQLLSDRCLQVLNRHLTVLRVVQLQPLQQVNLILSNNRGCRTLLLKIPKEYDLVLLFSSEEERGAFVQQLWDFCVRWALGLHVAEMSEKELFRKAVTKQQRERILEIFFRHLFAQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEDKSRLMFTMYDLDENGFLSKDEFFTMMRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSELRFTQLCVKGGGGGGNGIRDIFKQNISCRVSFITRTPGERSHPQGLGPPAPEAPELGGPGLKKRFGKKAAVPTPRLYTEALQEKMQRGFLAQKLQQYKRFVENYRRHIVCVAIFSAICVGVFADRAYYYGFASPPSDIAQTTLVGIILSRGTAASVSFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRWIAMAAVVLAILHSAGHAVNVYIFSVSPLSLLACIFPNVFVNDGSKLPQKFYWWFFQTVPGMTGVLLLLVLAIMYVFASHHFRRRSFRGFWLTHHLYILLYALLIIHGSYALIQLPTFHIYFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTYLQFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAVGPWTTRLREIYSSPKGNGCAGYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSLGSQMLCKKIYFIWVTRTQRQFEWLADIIQEVEENDHQDLVSVHIYVTQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLVNRQDRAHFMHHYENF 7BAA2350EC0A2A7E duox1_canlf Dual oxidase 1 DUOX1 THOX1 NADPH thyroid oxidase 1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGFCLALTWTFLVGSWTSMGAQKPISWEVQRFDGWYNNLMEHKWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNAAMRGPAGQASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPVFDPNGRGDVVLPFQRSRWDPESGQSPSNPRDLTNAVTGWLDGSAIYGSSHSWSDALRSFSGGQLASGPDPAFPRNAQPPLLMWSAPDPASGQRGPGGLYAFGAERGNRDPFLQALGLLWFRYHNLCAQRLARQHPHWGDEELFQHARKRVIATYQNIALYEWLPSFLQQAPVKYAGYNPFLDPSISPEFLVASEQFFSTMVPPGIYMRNASCHFQEVINRNSSISRALRVCNSYWSRKHPNLRRAEDVDALLLGMASQIAEREDHVVVEDVLDFWPGSLKFSRTDHVAGCLQRGRDLGLPSYTKARAALGLPPITRWQDINPALSQNNHTVLEATAALYNQDLSQLELLPGGLLESHGDPGPLFSAIVLNQFVRLRDGDRYWFENTRNGLFSEEEIAEIRNTSLRDVLVAVTNMNPSTLQPNVFFWHMGDPCPQPRQLSTQGLPACAPSTMQDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRKKNFKKLQGQDRKSVMSEKLVGGMEALEWQGHKEPCRPVLVHLQPGQICVVDGRLSVLRTIQLRPPQQVNLILSGNRGRRALLLKIPKEYDLVLLFNLEEERQVLVENLRGALKESGLKFQEWELREQELMRTAVTRQQRSHLLETFFRHLFSQVLDIDQADAGTLPLDSSQKVQEALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLTEVVESMFRESGFQDKEELTWEDFHFMLRDHDSELRFTQLCVRGVEVPEVIKDLCRRASYISQEKICPSPRVSARCPHSNTEVEWTPQRLQCPVDTDPPQEIRRRFGKKVTSFQPLLFTEAQREKFQRSRRHQTLQQFKRFIENYRRHIGCVAVFYAITGGLFLERAYYYAFGAHHMGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHNDGSEFPQKYYWWFFQTVPGLTGVMLLLVLAIMYVFASHHFRRHSFRGFWLTHHLYILLYVLLIIHGSFGLIQLPRFHIFFLVPALIYVGDKLVSLSRKKVEISVVKAELLPSGVTHLQFQRPQGFEYKSGQWVQIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSPPTGDGCAKYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDCQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFKSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF 94F7E93F34C9F1EE f1pld0_canlf TXNDC11 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MRAFLGLTIICPHVLCAEHSSSRAKDVIIPAKPPVSFFSSRSPVLDLFQGQLDYAEHVRRDSEVVLLFFYAPWCGQSIAARSEIEQAASQLSDQVLFVAINCWWNQGKCRKQKHFFYFPVIYLYHRSFGPIEYKGPMSAVYIEKFVRRVMKPLLYIPSQSELLDFLSNYEPGVLGYFEFSGSPQPPGYLTFFTSALHSLKKDYLGTVRFGVITNKHLAKLVSLVHSGSVYLHRHFNTSLVFPREAVNYTAENIHKWALENREALLRWLRPHGGKSLLLNNELKKGPALLVFLPFNPLAESHPLIDEITEVALEYNNCHGDQVVEQLLQHLRRADAPLLRPLAPEPPEQLPEPPLITASPCCNTVVLPQWHSVSRTHNVCELCVNQSTGGVRPSSVGMPQCSFFEMAAALDSFYLKEQTFYHVASDSIECSNFLSSYSPFSYYTACCRTINRAVTGFIDSGQSVFETPTITFSSLEKKCEVDPPGSVPHIEENRYLFPEVDVSSTNFTGLSCRTNKTLNIYLLDSNLFWLYAERLGAPSATRVKEFATIVDVKEESHYILDPKQALMKFTLESFIQNFSVLYSPLKRHLIGSDSAQFPSQHLITEVTTDTFWEVVLQKQDVLLLYYAQWCGFCPSLNHVFIQLARLLPSDTFTVARIDVSQNDLPWEFMVDRLPTVLFFPCNRKDRSVKYPEDLPVTLPNLLRFILHHSDPASTPQNLANPPTKECLQSEAVFQQGHISHLEREIQKLRAEISTLQRAQVQVEAQLSSARRDEHRLLRQKQTLEKQHSLLQLHSEQLQTLYEQKTHELEEVAQKLQELADASENLLTENTWLKILVATMERKLEGKDGAENLTPHKEAGSGHPNPSGVPRLPGGSPPPSNSSSTLASERSNENRTD 2C7E5B171B068395 duox1_human Dual oxidase 1 DUOX1 DUOX LNOX1 THOX1 Large NOX 1 Long NOX 1 NADPH thyroid oxidase 1 Thyroid oxidase 1 protein protein human Homo sapiens Human MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF 37CF124A579446B0 txd11_human Thioredoxin domain-containing protein 11 EF-hand-binding protein 1 TXNDC11 EFP1 protein protein human Homo sapiens Human MSECGGRGGGSSSSEDAEDEGGGGGGPAGSDCLSSSPTLATASSAGRLRRGLRGAFLMARQRPELLCGAVALGCALLLALKFTCSRAKDVIIPAKPPVSFFSLRSPVLDLFQGQLDYAEYVRRDSEVVLLFFYAPWCGQSIAARAEIEQAASRLSDQVLFVAINCWWNQGKCRKQKHFFYFPVIYLYHRSFGPIEYKGPMSAVYIEKFVRRVMKPLLYIPSQSELLDFLSNYEPGVLGYFEFSGSPQPPGYLTFFTSALHSLKKALESTSSPRALVSFTGEWHLETKIYVLDYLGTVRFGVITNKHLAKLVSLVHSGSVYLHRHFNTSLVFPREVLNYTAENICKWALENQETLFRWLRPHGGKSLLLNNELKKGPALFLFIPFNPLAESHPLIDEITEVALEYNNCHGDQVVERLLQHLRRVDAPVLESLALEVPAQLPDPPTITASPCCNTVVLPQWHSFSRTHNVCELCVNQTSGGMKPSSVSVPQCSFFEMAAALDSFYLKEQTFYHVASDSIECSNFLTSYSPFSYYTACCRTISRGVSGFIDSEQGVFEAPTVAFSSLEKKCEVDAPSSVPHIEENRYLFPEVDMTSTNFTGLSCRTNKTLNIYLLDSNLFWLYAERLGAPSSTQVKEFAAIVDVKEESHYILDPKQALMKLTLESFIQNFSVLYSPLKRHLIGSGSAQFPSQHLITEVTTDTFWEVVLQKQDVLLLYYAPWCGFCPSLNHIFIQLARNLPMDTFTVARIDVSQNDLPWEFMVDRLPTVLFFPCNRKDLSVKYPEDVPITLPNLLRFILHHSDPASSPQNVANSPTKECLQSEAVLQRGHISHLEREIQKLRAEISSLQRAQVQVESQLSSARRDEHRLRQQQRALEEQHSLLHAHSEQLQALYEQKTRELQELARKLQELADASENLLTENTWLKILVATMERKLEGRDGAESLAAQREVHPKQPEPSATPQLPGSSPPPANVSATLVSERNKENRTD 4A8C852F8E81B8BC duox1-txndc11-1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 14 physical association physical association no figure duox1-txndc11-2 2 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 19 physical association physical association 1 duox1-txndc11-3 2 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 24 22 physical association physical association 1 duox1-txndc11-4 1 9 anti tag immunost anti tag immunostaining anti tag immunost immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection colocalization colocalization 5 duox1-txndc11-5 4 9 anti tag immunost anti tag immunostaining anti tag immunost anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection physical association physical association 6B abd C Presence of EGTA or Ca(2+) or both does not affect association duox2-txndc11-1 4 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 9 anti tag immunost anti tag immunostaining anti tag immunost anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6B