DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS biology 2004 imex curation Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110955X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS biology 2004 imex curation Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110954X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS biology 2004 imex curation Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110951X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS biology 2004 imex curation Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110953X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS biology 2004 imex curation Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110952X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS biology 2004 imex curation Holaska JM., Kowalski AK., Wilson KL. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Holaska JM., Kowalski AK., Wilson KL. DIP original experiment: DIP-110950X PLoS biology 2004 IMEx Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. emd_human Emerin EDMD STA EMD protein protein human Homo sapiens Human MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF DIP protein P50402 original sequence version: 95 DIP protein P50402 original sequence version: 125 EB62EDD59B7A044F acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P60709 original sequence version: null DIP protein P60709 original sequence version: 63 DIP protein P60709 original sequence version: 128 DIP protein P60709 original sequence version: 129 DIP protein P60709 original sequence version: 58 DIP protein P60709 original sequence version: 66 DIP protein P60709 original sequence version: 88 DIP protein P60709 original sequence version: 89 DIP protein P60709 original sequence version: 86 DIP protein P60709 original sequence version: 138 DIP protein P60709 original sequence version: 112 6AFD05CA94E360E2 emd-actb-1 6 7 unspecified role unspecified role bait bait Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association acta1-emd-1 1 8 unspecified role unspecified role bait bait rabit Oryctolagus cuniculus (Rabbit) Rabbit 7 unspecified role unspecified role prey prey Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P50402:p.Gln133His Q133H mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q H This feature has been corrected as a result of our quality control procedures. The original label was 'Q133H'. Wed May 02 17:36:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln133his' Tue Jun 30 18:39:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln133His' ecolx Escherichia coli direct interaction direct interaction acta1-emd-2 4 8 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit 7 unspecified role unspecified role unspecified role unspecified role Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction emd-actb-2 3 7 unspecified role unspecified role bait bait human Homo sapiens Human 9 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association acta1-15 2 8 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction emd-acta1-1 5 8 unspecified role unspecified role prey prey rabit Oryctolagus cuniculus (Rabbit) Rabbit 7 unspecified role unspecified role bait bait Region 1-222 Region 1-222 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction