IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Nature genetics 2004 imex curation Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. jranish@systemsbiology.org 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. jranish@systemsbiology.org Accepted 2017-MAY-16 AT 12:29 BST AT 12:29 BST by MGT Nature genetics 2004 Only protein-protein interactions imex curation imex curation No this is definitely Fig. 4. I'd missed the fact that 5D was an IP, however so now added. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Nature genetics 2004 imex curation Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. jranish@systemsbiology.org 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. Complex pre-purified by anti-flag coimmunoprecipitation jranish@systemsbiology.org Accepted 2017-MAY-16 AT 12:29 BST AT 12:29 BST by MGT Nature genetics 2004 Only protein-protein interactions imex curation imex curation No this is definitely Fig. 4. I'd missed the fact that 5D was an IP, however so now added. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Nature genetics 2004 imex curation Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. jranish@systemsbiology.org 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. jranish@systemsbiology.org Accepted 2017-MAY-16 AT 12:29 BST AT 12:29 BST by MGT Nature genetics 2004 Only protein-protein interactions imex curation imex curation No this is definitely Fig. 4. I'd missed the fact that 5D was an IP, however so now added. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Nature genetics 2004 imex curation Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. jranish@systemsbiology.org 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R., Ranish JA., Hahn S., Lu Y., Yi EC., Li XJ., Eng J., Aebersold R. I've not curated Fig 1 as only 1 protein out of 209 is given - also Supp Fig 1 does not contain the rest. Immunoprecipitated proteins from the two samples (tagged and untagged) were differentially labeled with ICAT reagents and their relative abundance measured. Only those proteins shown to be significantly enriched are listed (P < 0.01).. RPL18A/B and RPS23A/B were also identified but could not be ascribed to a single gene product. jranish@systemsbiology.org Accepted 2017-MAY-16 AT 12:29 BST AT 12:29 BST by MGT Nature genetics 2004 Only protein-protein interactions imex curation imex curation No this is definitely Fig. 4. I'd missed the fact that 5D was an IP, however so now added. tfb5_yeast General transcription and DNA repair factor IIH subunit TFB5 TFB5 RNA polymerase II transcription factor B subunit 5 YDR079C-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MARARKGALVQCDPSIKALILQIDAKMSDIVLEELDDTHLLVNPSKVEFVKHELNRLLSKNIYNPMDEEENQ 9198E5CCFF304239 9198E5CCFF304239 utp8_yeast U3 small nucleolar RNA-associated protein 8 UTP8 t-UTP8 U three protein 8 U3 protein 8 required for transcription YGR128C protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSLSQPFRLATLPKIASLSNFSLQADYVQVADGTFNESTNNITLGISGSSISQYIINPTPKLTFDYPIPSTNIITACNAEKGQANIDGNIEASTDDEANNEKTINTQKKRNVEIWAFGLMVNKGNYTLNVITKALEDTTDTSNDHLSESDIDNKAYTGSDEFLSQYKIKAKAKVMSIKIDTKNSLVIAILQNGLIEIFDFKLTLLHSFDISYDNLKYAKWFTENGTEYVFVLCPLQDDKVCYKLLELTDCGSGESSPIKELSSTIIEGFSFENSKLCYQFGKLYKLNQGKIYIYSLPHCQLQQVIEFPMVDKLSPGDDLISFQPVSVNRVLLTVNNVIYLLDLLHCSTLSQRELTHVKTFQLLKSAVINSEKSHNSKTIAIGISTKNGPNPTSSLEIINIDVGTNTLKDSLGKSFQVGNNDSSVILKPLFDDKDINDKRVKCNDVSGDSSVPVLHCNEVIEKLSALQDNDITSFDDIFFKELKIKEEHYTEKDRYISDPGFLNKVLDLIFGKFSGNDYPKTLTFLLTHPLFPLSRTRNLLSLLRDQPRLFKQAIVTCPNLPLNELLEELFSIRNRELLLDISFRILQDFTRDSIKQEMKKLSKLDVQNFIEFITSGGEDSSPECFNPSQSTQLFQLLSLVLDSIGLFSLEGALLENLTLYIDKQVEIAERNTELWNLIDTKGFQHGFASSTFDNGTSQKRALPTYTMEYLDI 6F5AE1492F841F49 6F5AE1492F841F49 tfb3_yeast RNA polymerase II transcription factor B subunit 3 YDR460W RNA polymerase II transcription factor B 38 kDa subunit RNA polymerase II transcription factor B p38 subunit TFB3 RIG2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLMDEYEENKDMCPICKTDRYLSPDVKFLVNPECYHRICESCVDRIFSLGPAQCPYKGCDKILRKNKFKTQIFDDVEVEKEVDIRKRVFNVFNKTIDDFNGDLVEYNKYLEEVEDIIYKLDHGIDVAKTEEKLRTYEELNKQLIMNNLERSRTEIESFEQRQKFEKEMKLKKRLLERQIEEEERMNKEWTKKEIVNRLSTTTQDINETIEGVKNTVKLKKSSARRKLEELNRVLKNNPYFNSNVNVQNSRLKDAVPFTPFNGDREAHPRFTLKGSVYNDPFIKDLEHRKEFIASGFNTNYAYERVLTEAFMGLGCVISEEL 528D61CF25ADFAF4 528D61CF25ADFAF4 tfb2_yeast General transcription and DNA repair factor IIH subunit TFB2 RNA polymerase II transcription factor B subunit 2 YPL122C TFB2 RNA polymerase II transcription factor B p52 subunit RNA polymerase II transcription factor B 52 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDYSLKHSVTQYLEEIPQQVQNRLYTSPATCLAIYRILPPLAKFFIMAMVFNENEVPLLDLDKWVNSNGKLQFQNAIKSMKSLHLLIPNKSSGTLMINLNPTFKISLRNALTGGEVQNSFGVVVEENVVSLDLLDEYSANKWETILHFMVGTPLAKIPSEKVLNLLKHSKLMEEVNSTGEFKITNEGFQFLLQEINSQLWTLLLQYLKMIETSKMDLVDVLHFIFMLGALEVGKAYKIDALSETQRIMLQDMRDYGLVFQKHSNDSIFYPTKLALMLTSDTKTIRSASNAMDSVLRQNREEPSVNEDGANGKSTTDITTSDDLNKAGLKNQDIPDGSLIVETNFKIYSYSNSPLQIAVLSLFVHLKARFVNMVLGQITRESIRRALTNGITADQIIAYLETHAHPQMRRLAEEKLEKKLELDPNCKEPLQVLPPTVVDQIRLWQLELDRVITYEGSLYSDFETSQEYNLLSKYAQDIGVLLWKDDKKKKFFISKEGNSQVLDFAKRKLKKKQ 9E440B566396A95A 9E440B566396A95A uba4_yeast Adenylyltransferase and sulfurtransferase UBA4 YHR111W UBA4 NCS3 Ubiquitin-like protein activator 4 Needs CLA4 to survive protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNDYHLEDTTSELEALRLENAQLREQLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNYILDCTDSPLTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPPPNAVTSCQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSGFPQQSLRTFKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNVCEPDERISVDAFQRIYKDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIKNLRDMNGDLKKLQEKLPSVEKDSNIVILCRYGNDSQLATRLLKDKFGFSNVRDVRGGYFKYIDDIDQTIPKY CF686FCA74D29F30 CF686FCA74D29F30 tfb1_yeast General transcription and DNA repair factor IIH subunit TFB1 TFB1 RNA polymerase II transcription factor B p73 subunit RNA polymerase II transcription factor B 73 kDa subunit RNA polymerase II transcription factor B subunit 1 D9740.3 YDR311W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHSGAAIFEKVSGIIAINEDVSPAELTWRSTDGDKVHTVVLSTIDKLQATPASSEKMMLRLIGKVDESKKRKDNEGNEVVPKPQRHMFSFNNRTVMDNIKMTLQQIISRYKDADIYEEKRRREESAQHTETPMSSSSVTAGTPTPHLDTPQLNNGAPLINTAKLDDSLSKEKLLTNLKLQQSLLKGNKVLMKVFQETVINAGLPPSEFWSTRIPLLRAFALSTSQKVGPYNVLSTIKPVASSENKVNVNLSREKILNIFENYPIVKKAYTDNVPKNFKEPEFWARFFSSKLFRKLRGEKIMQNDRGDVIIDRYLTLDQEFDRKDDDMLLHPVKKIIDLDGNIQDDPVVRGNRPDFTMQPGVDINGNSDGTVDILKGMNRLSEKMIMALKNEYSRTNLQNKSNITNDEEDEDNDERNELKIDDLNESYKTNYAIIHLKRNAHEKTTDNDAKSSADSIKNADLKVSNQQMLQQLSLVMDNLINKLDLNQVVPNNEVSNKINKRVITAIKINAKQAKHNNVNSALGSFVDNTSQANELEVKSTLPIDLLESCRMLHTTCCEFLKHFYIHFQSGEQKQASTVKKLYNHLKDCIEKLNELFQDVLNGDGESMSNTCTAYLKPVLNSITLATHKYDEYFNEYNNNSN DAE369FE90A224F8 DAE369FE90A224F8 act_yeast Actin ACT1 ABY1 END7 YFL039C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF 87AC19B0B0BC9E71 87AC19B0B0BC9E71 alf_yeast Fructose-bisphosphate aldolase FBA1 YKL320 Fructose-1,6-bisphosphate aldolase YKL060C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGVEQILKRKTGVIVGEDVHNLFTYAKEHKFAIPAINVTSSSTAVAALEAARDSKSPIILQTSNGGAAYFAGKGISNEGQNASIKGAIAAAHYIRSIAPAYGIPVVLHSDHCAKKLLPWFDGMLEADEAYFKEHGEPLFSSHMLDLSEETDEENISTCVKYFKRMAAMDQWLEMEIGITGGEEDGVNNENADKEDLYTKPEQVYNVYKALHPISPNFSIAAAFGNCHGLYAGDIALRPEILAEHQKYTREQVGCKEEKPLFLVFHGGSGSTVQEFHTGIDNGVVKVNLDTDCQYAYLTGIRDYVLNKKDYIMSPVGNPEGPEKPNKKFFDPRVWVREGEKTMGAKITKSLETFRTTNTL C67E61BA5C7E8E4C C67E61BA5C7E8E4C rad3_yeast General transcription and DNA repair factor IIH helicase subunit XPD RAD3 REM1 DNA repair helicase RAD3 RNA polymerase II transcription factor B subunit RAD3 YER171W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGNSILEMPSGTGKTVSLLSLTIAYQMHYPEHRKIIYCSRTMSEIEKALVELENLMDYRTKELGYQEDFRGLGLTSRKNLCLHPEVSKERKGTVVDEKCRRMTNGQAKRKLEEDPEANVELCEYHENLYNIEVEDYLPKGVFSFEKLLKYCEEKTLCPYFIVRRMISLCNIIIYSYHYLLDPKIAERVSNEVSKDSIVIFDEAHNIDNVCIESLSLDLTTDALRRATRGANALDERISEVRKVDSQKLQDEYEKLVQGLHSADILTDQEEPFVETPVLPQDLLTEAIPGNIRRAEHFVSFLKRLIEYLKTRMKVLHVISETPKSFLQHLKQLTFIERKPLRFCSERLSLLVRTLEVTEVEDFTALKDIATFATLISTYEEGFLLIIEPYEIENAAVPNPIMRFTCLDASIAIKPVFERFSSVIITSGTISPLDMYPRMLNFKTVLQKSYAMTLAKKSFLPMIITKGSDQVAISSRFEIRNDPSIVRNYGSMLVEFAKITPDGMVVFFPSYLYMESIVSMWQTMGILDEVWKHKLILVETPDAQETSLALETYRKACSNGRGAILLSVARGKVSEGIDFDHQYGRTVLMIGIPFQYTESRILKARLEFMRENYRIRENDFLSFDAMRHAAQCLGRVLRGKDDYGVMVLADRRFSRKRSQLPKWIAQGLSDADLNLSTDMAISNTKQFLRTMAQPTDPKDQEGVSVWSYEDLIKHQNSRKDQGGFIENENKEGEQDEDEDEDIEMQ 978BCA01751949B4 978BCA01751949B4 tfb4_yeast General transcription and DNA repair factor IIH subunit TFB4 RNA polymerase II transcription factor B p34 subunit RNA polymerase II transcription factor B 34 kDa subunit TFB4 RNA polymerase II transcription factor B subunit 4 YPR056W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDAISDPTFKHARSRKQVTEESPSLLTVIIEIAPKLWTTFDEEGNEKGSIIKVLEALIVFLNAHLAFNSANKVAVIAAYSQGIKYLYPESTSALKASESENKTRSDLKIINSDMYRRFRNVDETLVEEIYKLFELEKKQIEQNSQRSTLAGAMSAGLTYVNRISKESVTTSLKSRLLVLTCGSGSSKDEIFQYIPIMNCIFSATKMKCPIDVVKIGGSKESTFLQQTTDATNGVYLHVESTEGLIQYLATAMFIDPSLRPIIVKPNHGSVDFRTSCYLTGRVVAVGFICSVCLCVLSIIPPGNKCPACDSQFDEHVIAKLKRKPVVPRLKAKKKVTKP B5C0CA95858453E0 B5C0CA95858453E0 ssl1_yeast General transcription and DNA repair factor IIH subunit SSL1 SSL1 RNA polymerase II transcription factor B subunit SSL1 Suppressor of stem-loop protein 1 YLR005W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAPVVISESEEDEDRVAITRRTKRQVHFDGEGDDRVDQQQQQHSSSHRDRDKHVQRKKKKRLSNRNLQGSNGGYAWEDEIKRSWDLVKVDDEGDMASLVASIVEARKKRTAKKNITPYQRGIIRSLILTLDCSEAMLEKDLRPNRHAMIIQYAIDFVHEFFDQNPISQMGIIIMRNGLAQLVSQVSGNPQDHIDALKSIRKQEPKGNPSLQNALEMARGLLLPVPAHCTREVLIVFGSLSTTDPGDIHQTIDSLVSEKIRVKVLGLSAQVAICKELCKATNYGDESFYKILLDETHLKELFNEAVTPLPVNKINKGFTLVKMGFPTRIFEDTPTFCSCHSKLVYGGYFCPNCHSKVCSLPTVCPCCDLMLILSTHLARSYHHLMPLKTFAEVPTTEKFRSEDCFSCQSRFPILKNHKNGKLLTSSRYRCEDCKQEFCVDCDVFIHEILHNCPGCESKPVIT 57ADCB630B790B4F 57ADCB630B790B4F pma1_yeast_gene Yeast PMA1 gene gene gene yeast Saccharomyces cerevisiae Baker's yeast act_yeast_gene Actin gene gene gene yeast Saccharomyces cerevisiae Baker's yeast leu1_yeast 2-isopropylmalate synthase LEU4 YNL104C N2173 Alpha-isopropylmalate synthase Alpha-IPM synthase protein protein yeast Saccharomyces cerevisiae Baker's yeast MVKESIIALAEHAASRASRVIPPVKLAYKNMLKDPSSKYKPFNAPKLSNRKWPDNRITRAPRWLSTDLRDGNQSLPDPMSVEQKKEYFHKLVNIGFKEIEVSFPSASQTDFDFTRYAVENAPDDVSIQCLVQSREHLIKRTVEALTGAKKATIHTYLATSDMFREIVFNMSREEAISKAVEATKLVRKLTKDDPSQQATRWSYEFSPECFSDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATVEVASPNVYADQIEYFATHITEREKVCISTHCHNDRGCGVAATELGMLAGADRVEGCLFGNGERTGNVDLVTVAMNMYTQGVSPNLDFSDLTSVLDVVERCNKIPVSQRAPYGGDLVVCAFSGSHQDAIKKGFNLQNKKRAQGETQWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVILRSLGLDLPRNMQIEFSSAVQDHADSLGRELKSDEISKLFKEAYNYNDEQYQAISLVNYNVEKFGTERRVFTGQVKVGDQIVDIEGTGNGPISSLVDALSNLLNVRFAVANYTEHSLGSGSSTQAASYIHLSYRRNADNEKAYKWGVGVSEDVGDSSVRAIFATINNIIHSGDVSIPSLAEVEGKNAAASGSA 6886E76C5DD89CE2 aro9_yeast Aromatic amino acid aminotransferase 2 ARO9 Aromatic amino acid aminotransferase II Aromatic amino acid-requiring protein 9 YHR137W Kynurenine aminotransferase I protein protein yeast Saccharomyces cerevisiae Baker's yeast MTAGSAPPVDYTSLKKNFQPFLSRRVENRSLKSFWDASDISDDVIELAGGMPNERFFPIESMDLKISKVPFNDNPKWHNSFTTAHLDLGSPSELPIARSFQYAETKGLPPLLHFVKDFVSRINRPAFSDETESNWDVILSGGSNDSMFKVFETICDESTTVMIEEFTFTPAMSNVEATGAKVIPIKMNLTFDRESQGIDVEYLTQLLDNWSTGPYKDLNKPRVLYTIATGQNPTGMSVPQWKREKIYQLAQRHDFLIVEDDPYGYLYFPSYNPQEPLENPYHSSDLTTERYLNDFLMKSFLTLDTDARVIRLETFSKIFAPGLRLSFIVANKFLLQKILDLADITTRAPSGTSQAIVYSTIKAMAESNLSSSLSMKEAMFEGWIRWIMQIASKYNHRKNLTLKALYETESYQAGQFTVMEPSAGMFIIIKINWGNFDRPDDLPQQMDILDKFLLKNGVKVVLGYKMAVCPNYSKQNSDFLRLTIAYARDDDQLIEASKRIGSGIKEFFDNYKS 8DA49174D59C8859 8DA49174D59C8859 adh1_yeast_gene Yeast ADH1 gene gene gene yeast Saccharomyces cerevisiae Baker's yeast rad25_yeast General transcription and DNA repair factor IIH helicase subunit XPB YIL143C SSL2 RAD25 UVS112 LOM3 Suppressor of stem-loop mutation 2 DNA repair helicase RAD25 RNA polymerase II transcription factor B subunit SSL2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDVEGYQPKSKGKIFPDMGESFFSSDEDSPATDAEIDENYDDNRETSEGRGERDTGAMVTGLKKPRKKTKSSRHTAADSSMNQMDAKDKALLQDTNSDIPADFVPDSVSGMFRSHDFSYLRLRPDHASRPLWISPSDGRIILESFSPLAEQAQDFLVTIAEPISRPSHIHEYKITAYSLYAAVSVGLETDDIISVLDRLSKVPVAESIINFIKGATISYGKVKLVIKHNRYFVETTQADILQMLLNDSVIGPLRIDSDHQVQPPEDVLQQQLQQTAGKPATNVNPNDVEAVFSAVIGGDNEREEEDDDIDAVHSFEIANESVEVVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAHAKLGLTATLVREDDKIGDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCPMTAEFYQEYLRETARKRMLLYIMNPTKFQACQFLIQYHERRGDKIIVFSDNVYALQEYALKMGKPFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMENIPNLAYASPRERRELLQEVLLKNEEAAGIEVGDDADNSVGRGSNGHKRFKSKAVRGEGSLSGLAGGEDMAYMEYSTNKNKELKEHHPLIRKMYYKNLKK FA4013E8156FE1C5 FA4013E8156FE1C5 tfb5-1 4 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association Fig 4, Supp Table 2 tfb1-tfb4-2 1 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5A tfb1-tfb4-3 2 10 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 5B YDR079c-a copurified with affinity-purified TFB4-TFB1 complexes on MonoQ. tfb5-leu4-1 4 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey association association Fig 5D tfb5-pma1_yeast_gene-1 3 16 unspecified role unspecified role prey prey promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3 tfb5-act_yeast_gene-1 3 17 unspecified role unspecified role prey prey promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3 tfb5-adh1_yeast_gene-1 3 20 unspecified role unspecified role prey prey promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3