UniProt UniProt http://www.uniprot.org YY1AP, a novel co-activator of YY1. YY1AP, a novel co-activator of YY1. The Journal of biological chemistry 2004 imex curation Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC., Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw Accepted 2017-MAY-16 AT 09:27 BST AT 09:27 BST by MGT The Journal of biological chemistry 2004 imex curation YY1AP, a novel co-activator of YY1. YY1AP, a novel co-activator of YY1. The Journal of biological chemistry 2004 imex curation Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC., Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw Accepted 2017-MAY-16 AT 09:27 BST AT 09:27 BST by MGT The Journal of biological chemistry 2004 imex curation YY1AP, a novel co-activator of YY1. YY1AP, a novel co-activator of YY1. The Journal of biological chemistry 2004 imex curation Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC., Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw Accepted 2017-MAY-16 AT 09:27 BST AT 09:27 BST by MGT The Journal of biological chemistry 2004 imex curation YY1AP, a novel co-activator of YY1. YY1AP, a novel co-activator of YY1. The Journal of biological chemistry 2004 imex curation Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC., Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw Accepted 2017-MAY-16 AT 09:27 BST AT 09:27 BST by MGT The Journal of biological chemistry 2004 imex curation YY1AP, a novel co-activator of YY1. YY1AP, a novel co-activator of YY1. The Journal of biological chemistry 2004 imex curation Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC., Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw Accepted 2017-MAY-16 AT 09:27 BST AT 09:27 BST by MGT The Journal of biological chemistry 2004 imex curation YY1AP, a novel co-activator of YY1. YY1AP, a novel co-activator of YY1. The Journal of biological chemistry 2004 imex curation Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC., Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang CY., Liang YJ., Lin YS., Shih HM., Jou YS., Yu WC. Winston@nhri.org.tw Accepted 2017-MAY-16 AT 09:27 BST AT 09:27 BST by MGT human HeLa S3 cDNA The Journal of biological chemistry 2004 imex curation tyy1_human Transcriptional repressor protein YY1 Yin and yang 1 Delta transcription factor NF-E1 YY1 INO80S INO80 complex subunit S protein protein human Homo sapiens Human MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEEEDDDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGGSSSSGGGRVKKGGGKKSGKKSYLSGGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ DIP protein P25490 original sequence version: 153 058C05A0AD2D04E6 yyap1_human YY1-associated protein 1 YY1AP1 HCCA2 YY1AP Hepatocellular carcinoma susceptibility protein Hepatocellular carcinoma-associated protein 2 protein protein human Homo sapiens Human MEEEASRSAAATNPGSRLTRWPPPDKREGSAVDPGKRRSLAATPSSSLPCTLIALGLRHEKEANELMEDLFETFQDEMGFSNMEDDGPEEEERVAEPQANFNTPQALRFEELLANLLNEQHQIAKELFEQLKMKKPSAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQIHLLATCNPNLNPEASSTRICLKELGTFAQSSIALHHQYNPKFQTLFQPCNLMGAMQLIEDFSTHVSIDCSPHKTVKKTANEFPCLPKQVAWILATSKVFMYPELLPVCSLKAKNPQDKILFTKAEDNKYLLTCKTARQLTVRIKNLNMNRAPDNIIKFYKKTKQLPVLGKCCEEIQPHQWKPPIEREEHRLPFWLKASLPSIQEELRHMADGAREVGNMTGTTEINSDQGLEKDNSELGSETRYPLLLPKGVVLKLKPVADRFPKKAWRQKRSSVLKPLLIQPSPSLQPSFNPGKTPAQSTHSEAPPSKMVLRIPHPIQPATVLQTVPGVPPLGVSGGESFESPAALPAMPPEARTSFPLSESQTLLSSAPVPKVMMPSPASSMFRKPYVRRRPSKRRGARAFRCIKPAPVIHPASVIFTVPATTVKIVSLGGGCNMIQPVNAAVAQSPQTIPIATLLVNPTSFPCPLNQPLVASSVSPLIVSGNSVNLPIPSTPEDKAHMNVDIACAVADGENAFQGLEPKLEPQELSPLSATVFPKVEHSPGPPPVDKQCQEGLSENSAYRWTVVKTEEGRQALEPLPQGIQESLNNSSPGDLEEVVKMEPEDATEEISGFL A2FC8B4DBA8DFDA7 q9h869-2 YY1-associated protein 1 YY1AP1 HCCA2 Hepatocellular carcinoma susceptibility protein Hepatocellular carcinoma-associated protein 2 YY1AP protein protein human Homo sapiens Human MEDLFETFQDEMGFSNMEDDGPEEEERVAEPQANFNTPQALRFEELLANLLNEQHQIAKELFEQLKMKKPSAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQIHLLATCNPNLNPEASSTRICLKELGTFAQSSIALHHQYNPKFQTLFQPCNLMGAMQLIEDFSTHVSIDCSPHKTVKKTANEFPCLPKQVAWILATSKVFMYPELLPVCSLKAKNPQDKILFTKAEDNLLALGLKHFEGTEFLNPLISKYLLTCKTARQLTVRIKNLNMNRAPDNIIKFYKKTKQLPVLGKCCEEIQPHQWKPPIEREEHRLPFWLKASLPSIQEELRHMADGAREVGNMTGTTEINSDQGLEKDNSELGSETRYPLLLPKGVVLKLKPVADRFPKKAWRQKRSSVLKPLLIQPSPSLQPSFNPGKTPAQSTHSEAPPSKMVLRIPHPIQPATVLQTVPGVPPLGVSGGESFESPAALPAMPPEARTSFPLSESQTLLSSAPVPKVMMPSPASSMFRKPYVRRRPSKRRGARAFRCIKPAPVIHPASVIFTVPATTVKIVSLGGGCNMIQPVNAAVAQSPQTIPIATLLVNPTSFPCPLNQPLVASSVSPLIVSGNSVNLPIPSTPEDKAHMNVDIACAVADGENAFQGLEPKLEPQELSPLSATVFPKVEHSPGPPPVDKQCQEGLSENSAYRWTVVKTEEGRQALEPLPQGIQESLNNSSPGDLEEVVKMEPEDATEEISGFL 35A05C3146FC95D7 yy1-yy1ap1-2 6 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association no figure yy1-yy1ap1-3 5 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait physical association physical association 3 yy1-yy1ap1-4 1 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 4 yy1-yy1ap1-5 3 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 5a yy1-yy1ap1-6 4 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5b yy1-yy1ap1-7 2 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 2B