UniProt UniProt http://www.uniprot.org Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Experimental cell research 1998 imex curation Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. kjetil.tasken@basalmed.uio.no 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions human-hs68 Homo sapiens Hs68 normal diploid foreskin fibroblasts hs68 Human Hs68 normal diploid foreskin fibroblasts fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. Mitotic cells have been used kjetil.tasken@basalmed.uio.no Accepted 2016-FEB-25 AT 08:53 GMT AT 08:53 GMT by ORCHARD Experimental cell research 1998 imex curation Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Experimental cell research 1998 imex curation Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. kjetil.tasken@basalmed.uio.no 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. kjetil.tasken@basalmed.uio.no Accepted 2016-FEB-25 AT 08:53 GMT AT 08:53 GMT by ORCHARD Experimental cell research 1998 imex curation Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Experimental cell research 1998 imex curation Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. kjetil.tasken@basalmed.uio.no 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K., Eide T., Coghlan V., Orstavik S., Holsve C., Solberg R., Skâlhegg BS., Lamb NJ., Langeberg L., Fernandez A., Scott JD., Jahnsen T., Taskén K. Mitotic cells have been used kjetil.tasken@basalmed.uio.no Accepted 2016-FEB-25 AT 08:53 GMT AT 08:53 GMT by ORCHARD Experimental cell research 1998 imex curation kap2_human cAMP-dependent protein kinase type II-alpha regulatory subunit PKR2 PRKAR2 PRKAR2A protein protein human Homo sapiens Human MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ DIP protein P13861 original sequence version: 107 DIP protein P13861 original sequence version: 128 C7D6E8E476E1DB65 akap8_human A-kinase anchor protein 8 AKAP8 AKAP95 A-kinase anchor protein 95 kDa protein protein human Homo sapiens Human MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEAAKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQDRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARERGSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSPSRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDGTGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEKEDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRFISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE DIP protein O43823 original sequence version: 118 CBCD5F014FD94B66 akap8-prkar2a-1 2 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 4C prkar2a-akap8-1 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 8B akap8-prkar2a-2 1 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization 7A