IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit. Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit. The EMBO journal 1996 imex curation Erickson FL., Hannig EM. 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay tag visualisation tag visualisation Erickson FL., Hannig EM. Accepted 2016-JUL-18 AT 08:48 BST AT 08:48 BST by MGT The EMBO journal 1996 Only protein-protein interactions imex curation imex curation As far as I can tell, 4A/B are identical - they are just counting 32P in A, and 3H in B. I've added an interaction with the cold GDP in each case - I'd just ignored it as part of the methodology previously. Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit. Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit. The EMBO journal 1996 imex curation Erickson FL., Hannig EM. 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast chromatography chromatography technology column chromatography chromatography weight by comassie molecular weight estimation by coomasie staining weight by comassie Erickson FL., Hannig EM. Cells were lysed, centrifuged, and ammonium sulphate precipitated (75%). The precipitate was resuspended and the complex sequentially purified on a Ni-column and a heparin-agarose column. Activity of the complex was confirmed , in addition to staining of the components. Accepted 2016-JUL-18 AT 08:48 BST AT 08:48 BST by MGT The EMBO journal 1996 Only protein-protein interactions imex curation imex curation As far as I can tell, 4A/B are identical - they are just counting 32P in A, and 3H in B. I've added an interaction with the cold GDP in each case - I'd just ignored it as part of the methodology previously. if2b_yeast Eukaryotic translation initiation factor 2 subunit beta YPL237W SUI3 TIF212 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSDLAAELGFDPALKKKKKTKKVIPDDFDAAVNGKENGSGDDLFAGLKKKKKKSKSVSADAEAEKEPTDDIAEALGELSLKKKKKKTKDSSVDAFEKELAKAGLDNVDAESKEGTPSANSSIQQEVGLPYSELLSRFFNILRTNNPELAGDRSGPKFRIPPPVCLRDGKKTIFSNIQDIAEKLHRSPEHLIQYLFAELGTSGSVDGQKRLVIKGKFQSKQMENVLRRYILEYVTCKTCKSINTELKREQSNRLFFMVCKSCGSTRSVSSIKTGFQATVGKRRRM 34DE9323876CEE95 34DE9323876CEE95 if2a_yeast Eukaryotic translation initiation factor 2 subunit alpha SUI2 TIF211 YJR007W J1429 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTSHCRFYENKYPEIDDIVMVNVQQIAEMGAYVKLLEYDNIEGMILLSELSRRRIRSIQKLIRVGKNDVAVVLRVDKEKGYIDLSKRRVSSEDIIKCEEKYQKSKTVHSILRYCAEKFQIPLEELYKTIAWPLSRKFGHAYEAFKLSIIDETVWEGIEPPSKDVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAAPLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATEDAELQALLESKELDNRSDSEDDEDESDDE AF4F1C80303A4E98 AF4F1C80303A4E98 gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 if2g_yeast Eukaryotic translation initiation factor 2 subunit gamma YER025W GCD11 TIF213 SUI4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLQDQEPSIIINGNLEPVGEPDIVEETEVVAQETQETQDADKPKKKVAFTGLEEDGETEEEKRKREFEEGGGLPEQPLNPDFSKLNPLSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDCYRSFKSDKEISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMREESALEHQKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAEIEDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKHWRLIGWATIKKGTTLEPIA D498AE62BC3E81CD D498AE62BC3E81CD trna imet initiator tRNA Met trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast AGCGCCGUGGCGCAGUGGAAGCGCGCAGGGCUCAUAACCCUGAUGUCCUCGGAUCGAAACCGAGCGGCGCUA gdp guanosine 5-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gcd11-sui2-8 3 5 unspecified role unspecified role prey prey P20459:p.Tyr142Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:42:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr142ala' Tue Jun 30 17:04:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr142Ala' P20459:p.Lys250Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Wed May 02 17:00:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys250arg' Tue Jun 30 17:32:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys250Arg' 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1 eif2_yeast-gdp-1 2 9 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Fig.3A A lys250arg mutation in the sequence of the gamma subunit of the complex (P32481) showed an approximately 30-fold increase in the koff value for GDP. eif2_yeast-gtp-1 2 6 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Fig. 3B, 4A Met-tRNAi(Met) appears to have a cooperative effect on GTP binding. eif2_yeast-trna imet-1 2 8 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role ancillary ancillary eif2 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait gtp binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 36 yeast Saccharomyces cerevisiae Baker's yeast direct interaction direct interaction Fig. 2A, 2B A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding eif2_yeast-trna imet-2 2 8 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role ancillary ancillary eif2 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait gtp binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeast Saccharomyces cerevisiae Baker's yeast direct interaction direct interaction Fig. 4A, 4B A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding eif2_yeast-gdp-2 2 9 competitor competitor competitor prey prey 9 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range direct interaction direct interaction Fig.3A A lys250arg mutation in the sequence of the gamma subunit of the complex (P32481) showed an approximately 30-fold increase in the koff value for GDP. eif2_yeast-gtp-2 2 6 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 competitor competitor competitor prey prey direct interaction direct interaction Fig. 3B, 4A Met-tRNAi(Met) appears to have a cooperative effect on GTP binding. eif2_yeast-gdp-3 2 9 competitor competitor competitor prey prey 8 competitor competitor competitor prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role ancillary ancillary eif2 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait gtp binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p32481 c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeast Saccharomyces cerevisiae Baker's yeast direct interaction direct interaction Fig. 4A, 4B A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding eif2_yeast IF-2 complex IF2 complex GCD11:SUI2:SUI3 eIF2 complex Eukaryotic translation initiation factor 2 complex eIF-2 complex 4 unspecified role unspecified role 5 unspecified role unspecified role 7 unspecified role unspecified role physical association physical association yeast Saccharomyces cerevisiae Baker's yeast stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B. Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis. Heterotrimer Molecular weight = 125 kDa Accepted 2016-JUN-08 by BMELDAL