IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Heterodimerization of the yeast MATa1 and MAT alpha 2 proteins is mediated by two leucine zipper-like coiled-coil motifs. Heterodimerization of the yeast MATa1 and MAT alpha 2 proteins is mediated by two leucine zipper-like coiled-coil motifs. The EMBO journal 1994 imex curation Ho CY., Adamson JG., Hodges RS., Smith M. 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ho CY., Adamson JG., Hodges RS., Smith M. The reference sequence for yeast is from strain S288c, which is a MAT alpha haploid. This means it does not have an active MAT A1 protein. The sequence found in UniProtKB for A1 is from other (MAT a) strains, therefore stored under the mnemonic YEASX, and not YEAST. Accepted 2016-SEP-08 AT 15:38 BST AT 15:38 BST by MGT The EMBO journal 1994 Only protein-protein interactions imex curation imex curation Heterodimerization of the yeast MATa1 and MAT alpha 2 proteins is mediated by two leucine zipper-like coiled-coil motifs. Heterodimerization of the yeast MATa1 and MAT alpha 2 proteins is mediated by two leucine zipper-like coiled-coil motifs. The EMBO journal 1994 imex curation Ho CY., Adamson JG., Hodges RS., Smith M. 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ho CY., Adamson JG., Hodges RS., Smith M. The reference sequence for yeast is from strain S288c, which is a MAT alpha haploid. This means it does not have an active MAT A1 protein. The sequence found in UniProtKB for A1 is from other (MAT a) strains, therefore stored under the mnemonic YEASX, and not YEAST. Accepted 2016-SEP-08 AT 15:38 BST AT 15:38 BST by MGT The EMBO journal 1994 Only protein-protein interactions imex curation imex curation mata1_yeasx Mating-type protein A1 MATA1 MAT1A protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MDDICSMAENINRTLFNILGTEIDEINLNTNNLYNFIMESNLTKVEQHTLHKNISNNRLEIYHHIKKEKSPKGKSSISPQARAFLEQVFRRKQSLNSKEKEEVAKKCGITPLQVRVWFINKRMRSK mint Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional corepressor that, in a/alpha diploid cells, binds cooperatively with the ALPHA2 protein to a 21-bp DNA sequence termed the haploid-specific gene (hsg) operator, to repress transcription of haploid-specific genes and of MATALPHA1 B413D0042EB97B3A mtal2_yeast Mating-type protein ALPHA2 MATALPHA2 ALPHA-2 MAT2A MATAL2 YCR039C YCR39C Alpha-2 repressor protein protein yeast Saccharomyces cerevisiae Baker's yeast MNKIPIKDLLNPQITDEFKSSILDINKKLFSICCNLPKLPESVTTEEEVELRDILGFLSRANKNRKISDEEKKLLQTTSQLTTTITVLLKEMRSIENDRSNYQLTQKNKSADGLVFNVVTQDMINKSTKPYRGHRFTKENVRILESWFAKNIENPYLDTKGLENLMKNTSLSRIQIKNWVSNRRRKEKTITIAPELADLLSGEPLAKKKE This sequence has been withdrawn from Uniprot. 30CD6A486D7D76CE 30CD6A486D7D76CE probe_pmid:8137824 EMSA probe PMID:8137824 ds dna double stranded deoxyribonucleic acid ds DNA ds dna yeast Saccharomyces cerevisiae Baker's yeast GCTTCCCAATGTAGAAAAGTACATCATAG matalpha2-mata1-1 1 3 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P0CY08:p.[Ile67Ala;Leu74Ala;Leu81Ala;Leu88Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Mon Oct 03 15:01:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ile57ala,leu74ala,leu81ala,leu88ala' Wed May 02 15:33:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67ala,leu74ala,leu81ala,leu88ala' Tue Jun 30 18:14:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile67Ala;Leu74Ala;Leu81Ala;Leu88Ala]' region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 5 matalpha2-probe_pmid:8137824-1 2 3 unspecified role unspecified role prey prey in vitro In vitro 4 unspecified role unspecified role prey prey P0CY08:p.Leu81Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L R Wed May 02 19:11:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu81arg' Tue Jun 30 18:14:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Arg' P0CY08:p.Leu81Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:27:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu81ala' Tue Jun 30 17:55:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Ala' P0CY08:p.[Leu74Ala;Leu81Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:33:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu74ala,leu81ala' Tue Jun 30 17:30:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu74Ala;Leu81Ala]' P0CY08:p.[Leu81Ala;Leu88Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 19:11:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu81ala,leu88ala' Tue Jun 30 18:19:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu81Ala;Leu88Ala]' P0CY08:p.[Leu88Ala;Ile95Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:26:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu88ala,ile95ala' Tue Jun 30 16:02:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu88Ala;Ile95Ala]' P0CY08:p.[Ile67Ala;Leu74Ala;Leu81Arg;Leu88Ala;Ile95Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 15:43:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67ala,leu74ala,leu81arg,leu88ala,ile95ala' Tue Jun 30 19:09:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile67Ala;Leu74Ala;Leu81Arg;Leu88Ala;Ile95Ala]' P0CY08:p.[Ile67Ala;Leu74Ala;Leu81Ala;Leu88Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:05:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67ala,leu74ala,leu81ala,leu88ala' Tue Jun 30 16:26:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile67Ala;Leu74Ala;Leu81Ala;Leu88Ala]' P0CY08:p.[Ile67Ala;Leu74Ala;Leu81Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:14:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67ala,leu74ala,leu81ala' Tue Jun 30 19:35:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile67Ala;Leu74Ala;Leu81Ala]' P0CY08:p.[Leu74Ala;Leu81Ala;Leu88Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:43:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu74ala,leu81ala,leu88ala' Tue Jun 30 18:20:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu74Ala;Leu81Ala;Leu88Ala]' P0CY08:p.[Leu81Ala;Leu88Ala;Ile95Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 15:25:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu81ala,leu88ala,ile95ala' Tue Jun 30 19:30:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu81Ala;Leu88Ala;Ile95Ala]' P0CY08:p.Cys33Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C F Wed May 02 19:03:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys33phe' Tue Jun 30 18:48:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys33Phe' P0CY08:p.Cys33Tyr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C Y Wed May 02 14:37:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys33tyr' Tue Jun 30 19:05:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys33Tyr' P0CY08:p.[Leu29Ile;Cys34Phe] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C F certain certain sequence position certain certain certain sequence position certain L I Thu Dec 08 14:32:45 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'cys34phe,leu29ile' Wed May 02 20:25:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu29ile,cys34phe' Tue Jun 30 17:44:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu29Ile;Cys34Phe]' P0CY08:p.Leu29Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L R Wed May 02 16:04:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu29arg' Tue Jun 30 19:36:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu29Arg' P0CY08:p.[Leu29Arg;Leu36Arg] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain L R Wed May 02 14:54:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu29arg,leu36arg' Tue Jun 30 15:55:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu29Arg;Leu36Arg]' in vitro In vitro 5 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figs. 4,6,7