NTNU NTNU, Norwegian University of Science and Technology www.ntnu.no/home A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome). A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome). Genes & development 1995 imex curation Steimle V., Durand B., Barras E., Zufferey M., Hadam MR., Mach B., Reith W. Walter.Reith@unige.ch 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa autoradiography autoradiography Steimle V., Durand B., Barras E., Zufferey M., Hadam MR., Mach B., Reith W. Walter.Reith@unige.ch Accepted 2016-MAY-16 AT 13:20 BST AT 13:20 BST by ORCHARD Genes & development 1995 Only protein-protein interactions imex curation imex curation A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome). A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome). Genes & development 1995 imex curation Steimle V., Durand B., Barras E., Zufferey M., Hadam MR., Mach B., Reith W. Walter.Reith@unige.ch 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift antibody detection identification by antibody antibody detection Steimle V., Durand B., Barras E., Zufferey M., Hadam MR., Mach B., Reith W. Walter.Reith@unige.ch Accepted 2016-MAY-16 AT 13:20 BST AT 13:20 BST by ORCHARD Genes & development 1995 Only protein-protein interactions imex curation imex curation dra_human_gene HLA class II histocompatibility antigen, DR alpha chain HLA-DRA HLA-DRA1 gene gene human Homo sapiens Human rfx5_human DNA-binding protein RFX5 RFX5 Regulatory factor X 5 protein protein human Homo sapiens Human MAEDEPDAKSPKTGGRAPPGGAEAGEPTTLLQRLRGTISKAVQNKVEGILQDVQKFSDNDKLYLYLQLPSGPTTGDKSSEPSTLSNEEYMYAYRWIRNHLEEHTDTCLPKQSVYDAYRKYCESLACCRPLSTANFGKIIREIFPDIKARRLGGRGQSKYCYSGIRRKTLVSMPPLPGLDLKGSESPEMGPEVTPAPRDELVEAACALTCDWAERILKRSFSSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKKPERLAQPPKDLEARTGAGPLARGERKKSVVESSAPGANNLQVNALVARLPLLLPRAPRSLIPPIPVSPPILAPRLSSGALKVATLPLSSRAGAPPAAVPIINMILPTVPALPGPGPGPGRAPPGGLTQPRGTENREVGIGGDQGPHDKGVKRTAEVPVSEASGQAPPAKAAKQDIEDTASDAKRKRGRPRKKSGGSGERNSTPLKSAAAMESAQSSRLPWETWGSGGEGNSAGGAERPGPMGEAEKGAVLAQGQGDGTVSKGGRGPGSQHTKEAEDKIPLVPSKVSVIKGSRSQKEAFPLAKGEVDTAPQGNKDLKEHVLQSSLSQEHKDPKATPP 5EBB33C677BB717F rfx5_hla-dra-1 1 3 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated xbox_promoter_region Xbox Promoter region of HLA-DRA sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined labeled_probe_Xbox Xbox Probe from HLA-DRA gene labeled with 32P 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated truncated_rfx5 Truncated RFX5 aa 1-195 binding region binding-associated region binding region n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain in vitro In vitro direct interaction direct interaction 4 hla_dra-rfx5-1 2 3 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated xbox_promoter_region Xbox Promoter region of HLA-DRA sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined labeled_probe_Xbox Xbox Probe from HLA-DRA gene labeled with 32P 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated rfx5_ha_tag RFX5 tagged with HA ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain direct interaction direct interaction 5B