IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells Journal of Experimental Medicine 2016 imex curation Roncagalli R Results from Figure 7 APs have not been included due to the presumed lack of specificity of the antibody to "CAPZ". not available free of charge until April 2017 I have a PDF sent by author - please ask me for it. However it now appears to be publically visible (December 2016) ... email sent 30/11 asking if any proteins/interactions are in Supp table S1 that are not in fig 2, and also Fig 6 if both RLTPR constructs are of Murine origin?-reply- both are mouse and All shown in fig 2. roncagalli@ciml.univ-mrs.fr, bernardm@ciml.univ-mrs.fr, malissen@ciml.univ-mrs.fr yes Only protein-protein interactions mouse-thymocyte MOUSE THYMOCYTE thymocyte THYMOCYTE affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom sequence tag sequence tag identification sequence tag Roncagalli R Results from Figure 7 APs have not been included due to the presumed lack of specificity of the antibody to "CAPZ". not available free of charge until April 2017 I have a PDF sent by author - please ask me for it. However it now appears to be publically visible (December 2016) ... email sent 30/11 asking if any proteins/interactions are in Supp table S1 that are not in fig 2, and also Fig 6 if both RLTPR constructs are of Murine origin?-reply- both are mouse and All shown in fig 2. Double positive CD4+CD8+ cells were lysed before or after treatment with the tyrosine-phosphatase inhibitor-Pervanadate, a surrogate for TCR stimulation. Proteins bound to RLTPR-OST were isolated using a Strep-Tactin-Sepharose beads, and eluted with D-biotin. roncagalli@ciml.univ-mrs.fr, bernardm@ciml.univ-mrs.fr, malissen@ciml.univ-mrs.fr Accepted 2017-FEB-01 AT 13:49 GMT AT 13:49 GMT by ORCHARD yes Journal of Experimental Medicine 2016 imex curation The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells Journal of Experimental Medicine 2016 imex curation Roncagalli R Results from Figure 7 APs have not been included due to the presumed lack of specificity of the antibody to "CAPZ". not available free of charge until April 2017 I have a PDF sent by author - please ask me for it. However it now appears to be publically visible (December 2016) ... email sent 30/11 asking if any proteins/interactions are in Supp table S1 that are not in fig 2, and also Fig 6 if both RLTPR constructs are of Murine origin?-reply- both are mouse and All shown in fig 2. roncagalli@ciml.univ-mrs.fr, bernardm@ciml.univ-mrs.fr, malissen@ciml.univ-mrs.fr yes Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom anti tag western anti tag western blot anti tag western Roncagalli R Results from Figure 7 APs have not been included due to the presumed lack of specificity of the antibody to "CAPZ". not available free of charge until April 2017 I have a PDF sent by author - please ask me for it. However it now appears to be publically visible (December 2016) ... email sent 30/11 asking if any proteins/interactions are in Supp table S1 that are not in fig 2, and also Fig 6 if both RLTPR constructs are of Murine origin?-reply- both are mouse and All shown in fig 2. Lysates of HEK293 cells coexpressing RLT PROST and RLT PRYPET molecules were subjected to AP with Strep-Tactin-Sepharose beads, followed by elution of proteins with D-biotin and subsequent Western blotting. The authors have confirmed that the constructs used were of Murine origin. roncagalli@ciml.univ-mrs.fr, bernardm@ciml.univ-mrs.fr, malissen@ciml.univ-mrs.fr Accepted 2017-FEB-01 AT 13:49 GMT AT 13:49 GMT by ORCHARD yes Journal of Experimental Medicine 2016 imex curation sit1_mouse Signaling threshold-regulating transmembrane adapter 1 Sit1 Sit SHP2-interacting transmembrane adapter protein Suppression-inducing transmembrane adapter 1 protein protein mouse Mus musculus Mouse MSRDYNCTTDDQLAWGIPSISHAWGLWALLGVVTVLLLISLAALLSQWTRGRRRNQEGQGPLSGRSAEEVPLYGNLHYLQTGRLSQEPRSEEQDPPSSGGLARGAEEAMCYTSLQLRPAQGRIPSSGNPIKYCEVVLDSEPKPQAPGPEPELYASVCAQTRRGRASFPDQAYANSQPAPS 7D1C0FD958B9BD03 dock8_mouse Dedicator of cytokinesis protein 8 Dock8 protein protein mouse Mus musculus Mouse MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSISTSGFPSLQLPQLYEPVEPVDFEGLVMTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSSEFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDERLENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLERESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDTSKSGGPTTVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLTGSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS E867E04339CC6009 sgt1_mouse Protein SGT1 homolog Sugt1 Suppressor of G2 allele of SKP1 homolog protein protein mouse Mus musculus Mouse MAAAAAGPASSQRLFQSFSDALIDGDPQAALEELTKALEQNPDDAQYYCQRAYCHILLGKYRDGIADVKKSLELNPNNCTALLRKGICEYHEKDYASALETFAEGQKLDSTDTNFDTWIKRCQEIQNGSESEVSASQRTQSKIKYDWYQTESHVIITLMIKSVQKNDVRVGFSERELSALVKIPAGEDYSLKLRLLHPIIPEQSTFKVLSTKIEIKMKKPEAVRWEKLEGQGDEPTPKQFTADVKNMYPSSSHYTRNWDKLVGEIKEEEKNEKLEGDAALNKLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKQY mint May play a role in ubiquitination and subsequent proteosomal degradation of target proteins 2A06CA65E9EB6289 car11_mouse Caspase recruitment domain-containing protein 11 Card11 CARD-containing MAGUK protein 1 Carma1 protein protein mouse Mus musculus Mouse MPGGGPAMDDYMETLKDEEEALWDNVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKLQQQVKAKDLQRCELLAKSRQLEDEKKQLSLIRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMRYAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPRKEQVLELERENEMLKTKIQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNKIYNLQEEVRQAEELRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKNDEMRIEMVRREACIVNLESKLRRLSKDNGSLDQSLPRHLPATIISQNLGDTSPRTNGQEADDSSTSEESPEDSKYFLPYHPPRRRMNLKGIQLQRAKSPISMKQASEFQALMRTVKGHEEDFTDGSPSSSRSLPVTSSFSKMQPHRSRSSIMSITAEPPGNDSIVRRCKEDAPHRSTVEEDNDSCGFDALDLDDENHERYSFGPPSIHSSSSSHQSEGLDAYDLEQVNLMLRKFSLERPFRPSVTSGGHVRGTGPLVQHTTLNGDGLITQLTLLGGNARGSFIHSVKPGSLAERAGLREGHQLLLLEGCIRGERQSVPLDACTKEEARWTIQRCSGLITLHYKVNHEGYRKLLKEMEDGLITSGDSFYIRLNLNISSQLDACSMSLKCDDVVHVLDTMYQDRHEWLCARVDPFTDQDLDTGTIPSYSRAQQLLLVKLQRLVHRGNREEADSAHHTLRSLRNTLQPEEMLSTSDPRVSPRLSRASFFFGQLLQFVSRSENKYKRMNSNERVRIISGSPLGSLSRSSLDATKLLTEKHEELDPENELSRNLTLIPYSLVRAFHCERRRPVLFTPTMLAKTLVQKLLNSGGAMEFTICKSDIVTRDEFLRKQKTETIIYSREKNPNTFECIVPANIEAVAAKNKHCLLEAGIGCVRDLIKCKVYPIVLLIRVSEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEAEMWSSVEELLRVLKDKIVEEQRKTIWVDEDQL DIP protein Q8CIS0 original sequence version: 70 2ED53905B99DD94E caza2_mouse F-actin-capping protein subunit alpha-2 Capza2 Cappa2 CapZ alpha-2 protein protein mouse Mus musculus Mouse MADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPYEAENAIESWRTSVETALRAYVKEHYPNGVCTVYGKKVDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTVTPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA DIP protein P47754 original sequence version: 85 E706A9BC1830E70B caza1_mouse F-actin-capping protein subunit alpha-1 CapZ alpha-1 Capza1 Cappa1 protein protein mouse Mus musculus Mouse MADFEDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYDDQVLITEHGDLGNSRFLDPRNQISFKFDHLRKEASDPQPEDVDGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPSAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSVTVSNEVQTTKEFIKIIESAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA 6FE97BCE5D9B9D3A cnot1_mouse CCR4-NOT transcription complex subunit 1 Cnot1 Kiaa1007 CCR4-associated factor 1 protein protein mouse Mus musculus Mouse MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSPDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIMQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSEGAQAHTWNVEVLIDVLKELNPSLNFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDFIYRPWKHAEGQLSFIQHSLINPEVFCFADYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQINTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILDVAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLAPEKDQPKSAQLPAETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASSLDAISPVQIDPLAGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGLSSQLPGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQSKMKPSDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVNTMVTTSTTTTVAKTVTVTKPTGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSLVFRPPNPWTMAIMNVLAELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPAITTTTTSTTPATSTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCITELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYRECHLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKILLVDERSVAHITEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS DIP protein Q6ZQ08 original sequence version: 42 DIP protein Q6ZQ08 original sequence version: 50 AfCS Y2H protein 39306. Translated from nucleotide sequence. 7771515027BA4B60 carl2_mouse Capping protein, Arp2/3 and myosin-I linker protein 2 Carmil2 Lrrc16c Rltpr Capping protein regulator and myosin 1 linker 2 F-actin-uncapping protein RLTPR Leucine-rich repeat-containing protein 16C RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein protein protein mouse Mus musculus Mouse MAQTPDDISCELRGEITRFLWPKEAELLLKTWLPQEGAEQSHILALLRWRAYLLHTCLPLRVDCTFSYLEVQAMALQETPPRVTFELESLPELVLEFPCVAALEQLAQHVAAAIKKVFPRSTLGKLFRKPTPSSLLARLERSHPLESTIPSSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFCLGDFSHFGSRDLALSVAALSYNLWFRRLSCEDMKLSLEVSEQILHMTSQSSYLEELVLEACGLRGDFVRRLAQALAGHFNSGLRELSLSGNLLDDRGMRALGRALATNATFDSTLTHLDLSGNPGALGPSQDSGGLYTFLSRPNVLAYLNLAGTDATLGTLFTALAGGCCSSLTHLEASRNIFSRMKSQAAPAALQRFLGGTRMLRHLGLAGCKLPPEALRALLEGLALNTQIHDLHLDLSACELRSVGAQVIQDLVCDAGALSSLDLSDNGFGSDMVTLVLAIGRSRSLKHVALGRNFNVRCKETLDDVLHRIAQLMQDDDCPLQSLSVAESRLKQGASILIRALGTNPKLTALDISGNAIGDAGAKMLAKALRVNTRLRSVIWDRNNTSALGLLDVAQALEQNHSLKSMPLPLNDVTQAHRSRPELTTRAVHQIQACLWRNNQVDSTSDLKPCLQPLGLISDHSEQEVNELCQSVQEHMELLGCGAGPQGEVAVHQAEDAIQNANFSLSILPILYEAGRSPSHHWQLQQKLESLLGQVGEICRQDIQDFTQTTLDTTRSLCPQMLQTPGWRKQLEGVLVGSGGLPELLPEHLLQDAFSRLRDMRLSITGTLAESIVAQALAGLHAARDRLVERLTQQAPVTMAPAVPPLGGNELSPLETGGLEELFFPTEKEEEREKVLLRKRNGTPSWQLRGKMQSRRLGRLHAVAEKHWAAGPRDTPASAVYQRVDVCVGWVPPALLQEGNGLTARVDEGVEEFFSKRLIQQDHFWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRPDLETSPGAAARARKSTLGDLLRPPARPGRGEEPGGAEGGTSSPDPARRNRPRYTRESKAYSMILLPAEEEAAVGTRPDKRRPLERGDTELAPSFEQRVQVMLQRIGVSRASGGAESKRKQSKDGEIKKAGSDGDIMDSSTETPPISIKSRTHSVSADPSCRPGPGGQGPESATWKTLGQQLNAELRGRGWGQQDGPGPPSPCPSPSPRRTSPAPDILSLPEDPCLGPRNEERPLRLQRSPVLKRRPKLEAPPSPSLGSGLGSKPLPPYPTEPSSPERSPPSPATDQRGGGPNP 9FB10DA18A26BCAA ptprc_mouse Receptor-type tyrosine-protein phosphatase C Ptprc Ly-5 Lymphocyte antigen 5 T200 Leukocyte common antigen protein protein mouse Mus musculus Mouse MTMGLWLKLLAFGFALLDTEVFVTGQTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAKALIIFLVFLIIVTSIALLVVLYKIYDLRKKRSSNLDEQQELVERDDEKQLMDVEPIHSDILLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKDARKPHNQNKNRYVDILPYDYNRVELSEINGDAGSTYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNKCAEYWPSMEEGTRAFKDIVVTINDHKRCPDYIIQKLNVAHKKEKATGREVTHIQFTSWPDHGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLEGLEAEGKVDVYGYVVKLRRQRCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHSCLHNMKKRDPPSDPSPLEAEYQRLPSYRSWRTQHIGNQEENKKKNRNSNVVPYDFNRVPLKHELEMSKESEPESDESSDDDSDSEETSKYINASFVMSYWKPEMMIAAQGPLKETIGDFWQMIFQRKVKVIVMLTELVNGDQEVCAQYWGEGKQTYGDMEVEMKDTNRASAYTLRTFELRHSKRKEPRTVYQYQCTTWKGEELPAEPKDLVSMIQDLKQKLPKASPEGMKYHKHASILVHCRDGSQQTGLFCALFNLLESAETEDVVDVFQVVKSLRKARPGVVCSYEQYQFLYDIIASIYPAQNGQVKKTNSQDKIEFHNEVDGGKQDANCVRPDGPLNKAQEDSRGVGTPEPTNSAEEPEHAANGSASPAPTQSS 44C3D35121181B31 grap2_mouse GRB2-related adaptor protein 2 GADS protein Growth factor receptor-binding protein GRBLG GRB-2-like protein Hematopoietic cell-associated adaptor protein GrpL GRB-2-related monocytic adapter protein Adapter protein GRID Grap2 Gads Grb2l Grid Mona protein protein mouse Mus musculus Mouse MEATAKFDFMASGEDELSFRTGDILKILSNQEEWLKAELGSQEGYVPKNFIDIEFPEWFHEGLSRHQAENLLMGKDIGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDTKGNYFLWTEKFPSLNKLVDYYRTTSISKQKQVFLRDGTQDQGHRGNSLDRRSQGGPHPSGTVGEEIRPSVNRKLSDHLPLGPQQFHPHQQPSPQFTPGPQPPQQQRYLQHFHQDRRGGSLDINDGHCGLGSEVNATLMHRRHTDPVQLQAAGRVRWARALYDFEALEEDELGFRSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMMR DIP protein O89100 original sequence version: 91 736311D0640CD3D0 fyb1_mouse FYN-binding protein 1 Fyb1 Adhesion and degranulation promoting adaptor protein FYB-120/130 FYN-T-binding protein Fyb SLAP-130 SLP-76-associated phosphoprotein protein protein mouse Mus musculus Mouse MAKFNTGSNPTEEAATSSRPFKVAGQSSPSGIQSRKNLFDNQGNASPPAGPSSMPKFGTTKPPLAAKPTYEEKPEKEPKPPFLKPTGGSPRFGTQPNSVSRDPEVKVGFLKPVSPKPTSLTKEDSKPVVLRPPGNKLHNLNQESDLKTPGPKPGPAPPVPENELKPGFSKVAGAKSKFMPAAQDTDSKPRFPRHTFGQKPSLSTEDSQEENTSKNVPVQKGSPVQLGAKSKGAPFKPPKEDPEDKDHGAPSSPFPGVVLKPAASRGSPGLSKNFEEKKEDRKTDLAKNIFLNKLNQEEPARFPKAPSKLTAGTPWGQSQEKEGDKNSATPKQKALPPLSVLGPPPPKPNRPPNVDLTRFRKADSANSATKSQTPYSTTSLPPPPPTHPASQPPLPASHPAHPPVPSLPPRNIKPPLDLKHPINDENQDGVMHSDGTGNLEEEQESEGETYEDIDSSKERDKKREKEEKKRLELERKEQKEREKKEQELKKKFKLTGPIQVIHHAKACCDVKGGKNELSFKQGEDIEIIRITDNPEGKWLGRTARGSYGYIKTTAVEIDYDSLKRKKNSLNAVPPRLVEDDQDVYDDVAEQDAPNSHGQSGSGGMFPPPPTDDEIYDGIEEEDDDDGSVPQVDEKTNAWSWGILKMLKGKDDRKKSIREKPKVSESDNNEGSSLPSQHKQLDVGEEVYDDVDASDFPPPPAEMSQGMSVGRAKTEEKDPKKLKKQEKEEKDLRKKFKYDGEIRVLYSTKVASSLTSKKWGARDLQIKPGESLEVIQSTDDTKVLCRNEEGKYGYVLRSYLVDNDGEIYDDIADGCIYDND DIP protein O35601 original sequence version: 105 mint Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2 6222CC7EF1CA2BBD ptprf_mouse Receptor-type tyrosine-protein phosphatase F Ptprf Lar Leukocyte common antigen related protein protein mouse Mus musculus Mouse MAPEPAPGRRMVPLVPALVMLGLMAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEDQLPSGFPTIDMGPQLKVVEKGRTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVMRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNTEPVSFYGIQYRAAGTDGPFQEVDGVASTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQANAESDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSDLGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPADSRNGIITQYSVAYEAVDGEDRKRHVVDGISREHSSWDLLGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVHVSWKLPVPNKQHGQIRGYQVTYVRLENGEPRGQPIIQDVMLAEAQETTISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMHTALLQWHPPKELPGELLGYRLQYRRADEARPNTIDFGKDDQHFTVTGLHKGATYVFRLAAKNRAGPGEEFEKEITTPEDVPSGFPQNLRVTGLTTSTTELTWDPPVLAERNGHITNYTVVYRDINSQLELQNVTNDTHLTLLGLKPDTTYDIKVRAHTSKGAGPLSPSIQSRTMPVEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPQKPLPASAFIEDGRFSLSMPQVQDPSLVRWFYIVVVPIDRVGGNLLAPRWNTPEELELDELLEAIEQGEEKQRRRRRQAERLKPYVAAQVDVLPDTFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPMDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNSEVNKPKNRYANVIAYDHSRVLLTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPVRGTETYGLIQVTLVDTVELATYTMRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAAMCGHTEVLARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFVSANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT 903FF6814F0BC914 grb2_mouse Growth factor receptor-bound protein 2 Grb2 Adapter protein GRB2 SH2/SH3 adapter GRB2 protein protein mouse Mus musculus Mouse MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQMPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 97F4A4FE4B248DDF cd8b_mouse T-cell surface glycoprotein CD8 beta chain Cd8b Cd8b1 Ly-3 Lymphocyte antigen 3 Lyt-3 Lyt3 T-cell membrane glycoprotein Ly-3 T-cell surface glycoprotein Lyt-3 protein protein mouse Mus musculus Mouse MQPWLWLVFSMKLAALWSSSALIQTPSSLLVQTNHTAKMSCEVKSISKLTSIYWLRERQDPKDKYFEFLASWSSSKGVLYGESVDKKRNIILESSDSRRPFLSIMNVKPEDSDFYFCATVGSPKMVFGTGTKLTVVDVLPTTAPTKKTTLKMKKKKQCPFPHPETQKGLTCSLTTLSLLVVCILLLLAFLGVAVYFYCVRRRARIHFMKQFHK mint 138D2DCA43215BDB cd28_mouse T-cell-specific surface glycoprotein CD28 Cd28 protein protein mouse Mus musculus Mouse MTLRLLFLALNFFSVQVTENKILVKQSPLLVVDSNEVSLSCRYSYNLLAKEFRASLYKGVNSDVEVCVGNGNFTYQPQFRSNAEFNCDGDFDNETVTFRLWNLHVNHTDIYFCKIEFMYPPPYLDNERSNGTIIHIKEKHLCHTQSSPKLFWALVVVAGVLFCYGLLVTVALCVIWTNSRRNRLLQSDYMNMTPRRPGLTRKPYQPYAPARDFAAYRP DIP protein P31041 original sequence version: 115 C8E1339DD7E53CA6 capzb_mouse F-actin-capping protein subunit beta Capzb Cappb1 CapZ beta protein protein mouse Mus musculus Mouse MSDQQLDCALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPPLEDGAMPSARLRKLEVEANNAFDQYRDLYFEGGVSSVYLWDLDHGFAGVILIKKAGDGSKKIKGCWDSIHVVEVQEKSSGRTAHYKLTSTVMLWLQTNKSGSGTMNLGGSLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIVNGLRSLDAIPDNHKFKQLQRELSQVLTQRQVYIQPDN DIP protein P47757 original sequence version: 91 C4409B1F9E014033 rasl3_mouse RAS protein activator like-3 Rasal3 protein protein mouse Mus musculus Mouse MKPECGQTMFRTFWSRSRDSSAMDPPLQSEEDSQTQPSLPSPLTSYRWHTGGSGEKAAGGFRWGRFAGWGRALSHQEPMVNSQPAPRSLFRRVLSAPPKESRSNRLRFSKTLWGRHKNVAPLEPKPNPKAPEPELELVADPDLPVAQIPEPPTPDMPVWNIDGFTLLEGKLVMLGEEEGPRQIRVGSASSENSMQAALGNLKDAVRTPGKTEPEAAGSNQVHNVRKLLKRLKEKKRAKSELGAYTPRDGPPSALGSRESLATLSELDLGAERDVRVWPLHPSLLGEPYCFQVTWAGGSLCFSCRSSAERDRWIEDLRRQFQPSQDNVERQEMWLTVWVHEAKGLPRATVPGVRAELWLDGALLARTAPRAGPGQLFWAERFHFEALPPARRLSLRLRSAGPAGATVGRVVLELDEVSIPRAPAAGLERWFPVLGAPAGAVLRARIRVRCLRVLPSERYKELAEFLTFHYARLCGALEPALSAQAKEELAAAMVRVLRATGRAQALVTDLGTAELARCGGREALLFRENTLATKAIDEYMKLVAQEYLQDTLGQVVRCLCASTEDCEVDPSKCPTPELPKHQARLRDSCEEVFENIIHSYNCFPAELGSVFSSWREACKARGSEALGPRLVCASLFLRLLCPAILAPSLFGLAPEHPAPGPARTLTLIAKVIQNLANCAPFGEKEAYMAFMNSFLEDHGPAMQHFLDQVATVDADTTPSGYQGSGDLALQLAVLHVQLCTIFAELDQKTQDSLEPLPTILRAIEEGRPVPVSVPMRLPRISTQVQSSFFSGEKPGFLAPRDLPKHTPLISKSQSLRSFQGAGSWASRRPDEERPQRRPRPVLRTQSVPARRPTHRRPSAGSKPRPKGSLRMGPAPCGRAWTRASASLPRKPSVPWQRQMDQPGDRYQTTGTHRPVGKLAEIQCEVAIFREAQKALSLLVESLSTQVQALKEQQEHFRCQLQDLYSRLGAGISKLDSKGGLPSNGSHRLKSLEQRLTEMECSQDQLRDSLQSLQLLSKTPGSRSQPLPLKAPCVNGADLSMGT DIP protein Q8C2K5 original sequence version: 108 DA58139782B1EEFF ptn6_mouse Tyrosine-protein phosphatase non-receptor type 6 Ptpn6 Hcp Hcph Ptp1C Protein-tyrosine phosphatase 1C Hematopoietic cell protein-tyrosine phosphatase 70Z-SHP SH-PTP1 PTPTY-42 protein protein mouse Mus musculus Mouse MVRWFHRDLSGPDAETLLKGRGVPGSFLARPSRKNQGDFSLSVRVDDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGILQDRDGTIIHLKYPLNCSDPTSERWYHGHISGGQAESLLQAKGEPWTFLVRESLSQPGDFVLSVLNDQPKAGPGSPLRVTHIKVMCEGGRYTVGGSETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMTTREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHKEEVYENVHSKSKKEEKVKKQRSADKEKNKGSLKRK DIP protein P29351 original sequence version: 154 DIP protein P29351 original sequence version: 102 DIP protein P29351 original sequence version: 117 DIP protein P29351 original sequence version: 164 DIP protein P29351 original sequence version: 134 CF17300D032638D2 ddx3x_mouse ATP-dependent RNA helicase DDX3X DEAD box protein 3, X-chromosomal DEAD box RNA helicase DEAD3 Embryonic RNA helicase D1Pas1-related sequence 2 Ddx3x D1Pas1-rs2 Ddx3 Dead3 Erh protein protein mouse Mus musculus Mouse MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRGDSRGKSSFFGDRGSGSRGRFDDRGRGDYDGIGGRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGAEIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEIDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGGSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN 216515CB00324017 vav_mouse Proto-oncogene vav Vav1 p95vav Vav protein protein mouse Mus musculus Mouse MELWRQCTHWLIQCRVLPPSHRVTWEGAQVCELAQALRDGVLLCQLLNNLLPQAINLREVNLRPQMSQFLCLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNKGIMPFPTEDSALNDEDIYSGLSDQIDDTAEEDEDLYDCVENEEAEGDEIYEDLMRLESVPTPPKMTEYDKRCCCLREIQQTEEKYTDTLGSIQQHFMKPLQRFLKPQDMETIFVNIEELFSVHTHFLKELKDALAGPGATTLYQVFIKYKERFLVYGRYCSQVESASKHLDQVATAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQDATEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLANYGRPKIDGELKITSVERRSKTDRYAFLLDKALLICKRRGDSYDLKASVNLHSFQVRDDSSGERDNKKWSHMFLLIEDQGAQGYELFFKTRELKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCYRCRAPAHKECLGRVPPCGRHGQDFAGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGIPPPPGAFGPFLRLNPGDIVELTKAEAEHNWWEGRNTATNEVGWFPCNRVHPYVHGPPQDLSVHLWYAGPMERAGAEGILTNRSDGTYLVRQRVKDTAEFAISIKYNVEVKHIKIMTSEGLYRITEKKAFRGLLELVEFYQQNSLKDCFKSLDTTLQFPYKEPERRAISKPPAGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRIGWFPSNYVEEDYSEYC DIP protein P27870 original sequence version: 120 DIP protein P27870 original sequence version: 141 3666DCCD1C5229DA carmil2-capza1-1 1 5 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait OST-modified_strep2_tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Figure 2 234q54ae-1 2 10 unspecified role unspecified role prey prey c_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role bait bait OST-modified_strep2_tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 6