HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Involvement of FKBP6 in hepatitis C virus replication. Involvement of FKBP6 in hepatitis C virus replication. Scientific reports 2014 imex curation Kasai H., Kawakami K., Yokoe H., Yoshimura K., Matsuda M., Yasumoto J., Maekawa S., Yamashita A., Tanaka T., Ikeda M., Kato N., Okamoto T., Matsuura Y., Sakamoto N., Enomoto N., Takeda S., Fujii H., Tsubuki M., Kusunoki M., Moriishi K. Virus - Publications including interactions involving viral proteins Interactions involving NS5A from HCV 1b starin N (Uniprot id Q9WPH5) were not annotated as this entry is only in TrEMBL at the time this paper was curated and the NS5A protein could not be specifically described. kmoriishi@yamanashi.ac.jp 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kasai H., Kawakami K., Yokoe H., Yoshimura K., Matsuda M., Yasumoto J., Maekawa S., Yamashita A., Tanaka T., Ikeda M., Kato N., Okamoto T., Matsuura Y., Sakamoto N., Enomoto N., Takeda S., Fujii H., Tsubuki M., Kusunoki M., Moriishi K. Virus - Publications including interactions involving viral proteins Interactions involving NS5A from HCV 1b starin N (Uniprot id Q9WPH5) were not annotated as this entry is only in TrEMBL at the time this paper was curated and the NS5A protein could not be specifically described. kmoriishi@yamanashi.ac.jp Accepted 2016-APR-27 AT 12:08 BST AT 12:08 BST by ORCHARD Scientific reports 2014 imex curation Involvement of FKBP6 in hepatitis C virus replication. Involvement of FKBP6 in hepatitis C virus replication. Scientific reports 2014 imex curation Kasai H., Kawakami K., Yokoe H., Yoshimura K., Matsuda M., Yasumoto J., Maekawa S., Yamashita A., Tanaka T., Ikeda M., Kato N., Okamoto T., Matsuura Y., Sakamoto N., Enomoto N., Takeda S., Fujii H., Tsubuki M., Kusunoki M., Moriishi K. Virus - Publications including interactions involving viral proteins Interactions involving NS5A from HCV 1b starin N (Uniprot id Q9WPH5) were not annotated as this entry is only in TrEMBL at the time this paper was curated and the NS5A protein could not be specifically described. kmoriishi@yamanashi.ac.jp 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kasai H., Kawakami K., Yokoe H., Yoshimura K., Matsuda M., Yasumoto J., Maekawa S., Yamashita A., Tanaka T., Ikeda M., Kato N., Okamoto T., Matsuura Y., Sakamoto N., Enomoto N., Takeda S., Fujii H., Tsubuki M., Kusunoki M., Moriishi K. Virus - Publications including interactions involving viral proteins Interactions involving NS5A from HCV 1b starin N (Uniprot id Q9WPH5) were not annotated as this entry is only in TrEMBL at the time this paper was curated and the NS5A protein could not be specifically described. kmoriishi@yamanashi.ac.jp Accepted 2016-APR-27 AT 12:08 BST AT 12:08 BST by ORCHARD Scientific reports 2014 imex curation Involvement of FKBP6 in hepatitis C virus replication. Involvement of FKBP6 in hepatitis C virus replication. Scientific reports 2014 imex curation Kasai H., Kawakami K., Yokoe H., Yoshimura K., Matsuda M., Yasumoto J., Maekawa S., Yamashita A., Tanaka T., Ikeda M., Kato N., Okamoto T., Matsuura Y., Sakamoto N., Enomoto N., Takeda S., Fujii H., Tsubuki M., Kusunoki M., Moriishi K. Virus - Publications including interactions involving viral proteins Interactions involving NS5A from HCV 1b starin N (Uniprot id Q9WPH5) were not annotated as this entry is only in TrEMBL at the time this paper was curated and the NS5A protein could not be specifically described. kmoriishi@yamanashi.ac.jp 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kasai H., Kawakami K., Yokoe H., Yoshimura K., Matsuda M., Yasumoto J., Maekawa S., Yamashita A., Tanaka T., Ikeda M., Kato N., Okamoto T., Matsuura Y., Sakamoto N., Enomoto N., Takeda S., Fujii H., Tsubuki M., Kusunoki M., Moriishi K. Virus - Publications including interactions involving viral proteins Source of FKBP8 inferred from PMID:17024179, FKBP6 from PMID:18529014 Interactions involving NS5A from HCV 1b starin N (Uniprot id Q9WPH5) were not annotated as this entry is only in TrEMBL at the time this paper was curated and the NS5A protein could not be specifically described. kmoriishi@yamanashi.ac.jp Accepted 2016-APR-27 AT 12:08 BST AT 12:08 BST by ORCHARD Scientific reports 2014 imex curation fkbp8_human Peptidyl-prolyl cis-trans isomerase FKBP8 FK506-binding protein 8 38 kDa FK506-binding protein FKBPR38 FKBP8 FKBP38 Rotamase protein protein human Homo sapiens Human MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN DIP protein Q14318 original sequence version: 145 887C25ADE71EBA8D fkbp6_human Inactive peptidyl-prolyl cis-trans isomerase FKBP6 FK506-binding protein 6 36 kDa FK506-binding protein Immunophilin FKBP36 FKBP6 FKBP36 protein protein human Homo sapiens Human MGGSALNQGVLEGDDAPGQSLYERLSQRMLDISGDRGVLKDVIREGAGDLVAPDASVLVKYSGYLEHMDRPFDSNYFRKTPRLMKLGEDITLWGMELGLLSMRRGELARFLFKPNYAYGTLGCPPLIPPNTTVLFEIELLDFLDCAESDKFCALSAEQQDQFPLQKVLKVAATEREFGNYLFRQNRFYDAKVRYKRALLLLRRRSAPPEEQHLVEAAKLPVLLNLSFTYLKLDRPTIALCYGEQALIIDQKNAKALFRCGQACLLLTEYQKARDFLVRAQKEQPFNHDINNELKKLASCYRDYVDKEKEMWHRMFAPCGDGSTAGES C137FA629B676623 q9wmx2-pro_0000037551 Non-structural protein 5A protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) SGSWLRDVWDWICTVLTDFKTWLQSKLLPRLPGVPFFSCQRGYKGVWRGDGIMQTTCPCGAQITGHVKNGSMRIVGPRTCSNTWHGTFPINAYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFLVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRRLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFEPLQAEEDEREVSVPAEILRRSRKFPRAMPIWARPDYNPPLLESWKDPDYVPPVVHGCPLPPAKAPPIPPPRRKRTVVLSESTVSSALAELATKTFGSSESSAVDSGTATASPDQPSDDGDAGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEASEDVVCC 1973 2419 AC8324B2DE0F7FAE ns5a-fkbp8-6 3 4 unspecified role unspecified role bait bait n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1B and 1C NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 ns5a-fkbp8-7 3 4 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1B NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 ns5a-fkbp6-1 3 5 unspecified role unspecified role bait bait n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1C NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 ns5a-fkbp6-2 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 1D NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 fkbp6-ns5a-1 1 5 unspecified role unspecified role prey prey purified purified n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait purified purified c terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1E NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 fkbp6-ns5a-2 3 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey Q9WMX2-PRO_0000037551:p.Val121Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Wed May 02 16:12:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val121ala' Tue Jun 30 15:36:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val121Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3A NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 fkbp6-ns5a-3 3 5 unspecified role unspecified role bait bait n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3C NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 ns5a-fkbp6-3 3 5 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3C NS5A from HCV 1b starin N (Uniprot id Q9WPH5) is also shown to interact with FKBP8 fkbp6-fkbp6-1 3 5 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A fkbp6-fkbp6-2 3 5 unspecified role unspecified role bait bait n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A fkbp8-fkbp6-1 3 4 unspecified role unspecified role bait bait n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A fkbp6-fkbp8-1 3 4 unspecified role unspecified role prey prey n terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A