UniProt UniProt http://www.uniprot.org The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PloS one 2014 imex curation Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp Accepted 2016-NOV-30 AT 11:33 GMT AT 11:33 GMT by MGT PloS one 2014 imex curation fig 4b exp with bait nemp1b (delta)TM and N constructs omitted as the wildtxpe nemp1b:nemp1b interaction has not been demonstrated. mail exchange with the author. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PloS one 2014 imex curation Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp Accepted 2016-NOV-30 AT 11:33 GMT AT 11:33 GMT by MGT mouse 11-day embryo MatchMaker cDNA library PloS one 2014 imex curation fig 4b exp with bait nemp1b (delta)TM and N constructs omitted as the wildtxpe nemp1b:nemp1b interaction has not been demonstrated. mail exchange with the author. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PloS one 2014 imex curation Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp Accepted 2016-NOV-30 AT 11:33 GMT AT 11:33 GMT by MGT PloS one 2014 imex curation fig 4b exp with bait nemp1b (delta)TM and N constructs omitted as the wildtxpe nemp1b:nemp1b interaction has not been demonstrated. mail exchange with the author. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PloS one 2014 imex curation Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. fig 4b exp with bait nemp1b (delta)TM and N constructs omitted as the wildtxpe nemp1b:nemp1b interaction has not been demonstrated. mail exchange with the author. Origin of Nemp1 and its constructs according Mamada et al., 2009 (PMID 19167377). Origin of Ran(delta)C contrsuct according Villa Braslavsky et al., 2000 (PMID 10995230). Use of Nemp1a or Nemp1b constructs in experimental set-ups confirmed by the author (T. Shibano) by e-mail. m_taira@bs.s.u-tokyo.ac.jp Accepted 2016-NOV-30 AT 11:33 GMT AT 11:33 GMT by MGT PloS one 2014 imex curation fig 4b exp with bait nemp1b (delta)TM and N constructs omitted as the wildtxpe nemp1b:nemp1b interaction has not been demonstrated. mail exchange with the author. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. The Inner Nuclear Membrane Protein Nemp1 Is a New Type of RanGTP-Binding Protein in Eukaryotes. PloS one 2014 imex curation Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. m_taira@bs.s.u-tokyo.ac.jp 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Shibano T., Mamada H., Hakuno F., Takahashi S., Taira M. Association between GST-RanQ69L and importin beta1 (and the inhibitory effect of Nemp1; fig 3F) omitted as origin of importin beta1 cannot be established from the publication. m_taira@bs.s.u-tokyo.ac.jp Accepted 2016-NOV-30 AT 11:33 GMT AT 11:33 GMT by MGT PloS one 2014 imex curation fig 4b exp with bait nemp1b (delta)TM and N constructs omitted as the wildtxpe nemp1b:nemp1b interaction has not been demonstrated. mail exchange with the author. ran_mouse GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Ran Rasl2-8 protein protein mouse Mus musculus Mouse MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL D5C9B7275C34BCE0 nemp1_mouse Nuclear envelope integral membrane protein 1 Nemp1 Kiaa0286 Tmem194 Tmem194a protein protein mouse Mus musculus Mouse MAGGIKVSVWSAVGPGPRCWGAGGGGGATWLLLVVAGCVVCGSADVNVVMLQESQVDMNSSQQFCYKNVLIPKWHDIWTRIQVRVNSSKLVRVTQVDNEEKLKELEQFSIWNFFSSFLKEKLNDTYVNVGLYSTKTCLKVEMIEKDTTYSVTVTRRFDPKLFLVFLLGLTLFFCGDLLSRSQIFYYSTGMSVGIVASLLIVIFMISKFMPKRSPIYVILVGGWSFSLYLIQLVFKNLQEIWRSYWHYLLSYILTVGFMSFAVCYKYGPLENERSINLLTWTLQLLGLGLMYSSIQIPHVAFALIVIALCTKNLEYPIHWLCSTYRRMCKASGKPVPPRLLTEEEYRIQGEVETQKALQELREFCNSPECSAWKTISRIQSPKRFADFVEGSFHLTPNEVSVHEQEYGLGSIFTQDEELSSEEEGSEYPTFTQNNFLT F6B25929100CC1D9 q9shq6_arath F1K23.6 F1K23_6 At1g28760 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTTSSSFLRLPHRIWFLLPLIVFLLLSYAAALKSVGPGEPALNISLRSCERIQVSGYSRTKLGKFANSLRVNLVPIPEKLHLKIQVCVHRNATLGMCHCEKSNWKNLQSSSVISPYDKQYIDVRFTGSEYQSSRSMITVNVTEEFHQWRIFCLVAGLIVILLAPVVSSWLPFYYTSSMAVGVFLVVLIIIFQVMRLLPTGRKNVMYLAFYGSVVGAGSFILHQFSMMVNMILVNFGLSEDMYNPVAILVLVGVVITGAAFGFWTVRKFVVSKDGGGVDASVAQFVKWAMRSVAATFILQSSLDTPMAMGAFVLASLFGFLVSKTKSFVGKSHWLVSAGERRPIMHGRAEFLSRPGGGGLWKSAKSVPSYSGSPSYDVIVSSPSSVNRRIPIGNQDYYSTFHTTPNRKRMSKREYDELTQETTREAMAGLAASPGFSDWLVEHADRIKLLPTDSSYDDELGSESDSTGEQSWTRFFW 6741940C0EAE02BE nmp1a_xenla Nuclear envelope integral membrane protein 1a nemp1a tmem194a-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAGDVEGGGCRVSWGALLTLLLLPLPSLCTLASGKEPHVIKLYEGKVVRYNESKNFCYQRTYEPKWSDVWTKIQIRVNSTKMIRVTQVENEEKLKEMETFNMFDLFSSFLKEKLNDTFIYVDLYSNKTCIKVHVIDTDTYYSVALSRGFDPRLCFLFLCGLLLFFYGDALSRSQLFFYSTGITIGMLASMLILVFMLSKLMPKKSPFVALLLGGWSVSIYIIQLVFKNLQAICSEYWQYLLGYLGIVGFVSFAFCYKYGPLENDRSINILTWTLQLIGLLLMYISVQIQHIAVTMVVIAFCTKQIEYPVQWIYILYRKIKRKRAKPSPPRLLTEEEYRKQGEIETRKALEELRGYCSSPDFATWKMISRIQSPKRFADFVEGSSHLTPNEVSVHEHEYGLGGSFLEDELFGEDSDIEVEMDIEQPLYLVPRSCF 26654FADC60FB8A1 nmp1b_xenla Nuclear envelope integral membrane protein 1b nemp1b tmem194a-b protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAGEVEGRGCGFSLGVLVTLLVLPLPSLCTLSTEKELHVIKLYEGRMVRYNESRNFCYQRTYEPKWSDVWTKIQIRINSTKMIRVTQVDNEEKLKEMETFNMFDFFSSFLKEKLNDTFIYVNLYSNKTCVKVHLTDTDTYYSVALSRGFDPRLFFVFLCGLLLFFYGDTLSRSQLFFYSTGITVGMLASMLILVFMLSKLMPKKSPFFALLLGGWSVSIYVIQLVFRNLQAICSEYWQYLIVYLGIVGFVSFAFCYIYGPLENERSINILNWTLQLIGLLLMYVSVQIQHIAVTIVVIAFCTKQIEYPVQWIYILYRKIKLKRAKPGPPRLLTEEEYRKQADVETRKALEELRECCSSPDFAAWKTISRIQSPKRFADFVEGSSHLTPNEVSVHEHEYGLGGSFLEDELFGEDSDVEEEMEIEPPLYPIPRSVF 258089C9B16BA011 q7ztb4_xenla lemd3.S XMAN1 lemd3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAAAQLTDEELFSELKRSGIMPGPVTDSTRPVYLKKLKKLREEQRLRHGKVRVITNNNTVAAPSESEGILKSGGDVTHKRYSRQSLAREAGKPSPILGFSSDESDVDAGLGAHGSRRSRTNTPQPERKSPVQHQPGTADANADRRKVPGWWEMRRPRGDSEEDDDDEEEGTEEASNCRTMNGSRHSYCRDYSDTDEEEQLGQDEQASRRSRRGSSKQAKEQSLDRDELAAWPGWSREVAGVSHRHRGAARKSHTAADTDGAGNLKRKSNNHLESGYNKDPPRVYFPSNSLPPRMPQSNHTAAPNHTKYSHYHYPDSKRQHHKVAEPEEALLQQFKRDEVSAAGRFSAHYLSMFLLTAACLFFLLLGLTYLRMRGTSLPANENLTSTEDLNVDSKKNLMNTLQKLHEKLSKTAGDYICGNTDHRNISVQEAKQYLRTLGQEYENEFNNTLAWILEHSATRDIGIKCVSDPGSILNITSVKYLESTLPKMSFMCRIKRAVITVAHRSSILLVGVGVLFGVWRYIKYRWAKEEEETKQMYDMVVKIIDVLKSHNEACQENKGLEPYTPIPHVRDSLILPQDRKKMKKVWDRAVEFLDANESRVRTETQKIGGADFQVWKWIQPSSTCDKISVMPSKVWQGQAFHLDRRNSPPNSLTPCLKIRNMFDPVMEIGDHWDLAIQEAILEKCSDNEGIVHIAVDKNSREGCVYVKCLSPEFAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTCSTPLKPSNTHMNSLLHLNLRTGTSNSQSCC 6387BEC34EE60EEB ran2_arath GTP-binding nuclear protein Ran-2 RAN2 Ras-related nuclear protein 2 F28I16.170 At5g20020 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALPNQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDQNLHFVESPALAPPEVHLDIAAQQQNEADLAAAAAQPLPDDDDDAFE 349390AC56E05AFB ubc9_mouse SUMO-conjugating enzyme UBC9 Ube2i Ubc9 Ubce9 Ubce2i SUMO-protein ligase Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I Ubiquitin carrier protein I Ubiquitin carrier protein 9 RING-type E3 SUMO transferase UBC9 protein protein mouse Mus musculus Mouse MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS DIP protein P63280 original sequence version: 30 E2C826E9C8D0683D emd_human Emerin EDMD STA EMD protein protein human Homo sapiens Human MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF DIP protein P50402 original sequence version: 95 DIP protein P50402 original sequence version: 125 EB62EDD59B7A044F ran_xenla GTP-binding nuclear protein Ran ran GTPase Ran Ras-like protein TC4 Ras-related nuclear protein protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEQDLQNAQATALPDEDDDL D5DB10D65C2AD5A4 f4k567_arath K9I9.18 K9I9_18 At5g67610 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGDYRLLRNSGVVIGIILLHLFTFASLVSSDELQFGVVVNFLWPYGTVVGESGELQVTPSLEVKGSPGLKPDRTSLCERIHIHGLGRFKHLDKYAHSLKLIVNASISGKTNNIDVCFHRNLSRGIGMCPHSRWEKASKGSWVQTMSPFDHKILDVRVPSSNKVSLEVSAVEELFMHRIVFLLLGAVLLASASTLSQSLAFYYSSAMAVGIILVVLLVLFQGMKLLPTGRSSFALFIYSTLLGLGGFLLRYLPGLFESLLTEMGIDEEMYTPAAIFVGAFLSLGGAFFGFWTVRKLILTEDGSIDVSTSLFVSWSIRIMAAVLILQSSVDPLLAGGALISVILMSSTLKKITRLKFLLRLFEIPLNLLLGIWEAIRDTDIPSVPGYLHDFMQKSPDASGFRNRVTSASPSGGINNGMRESPPSESDTFPSSFHKTPERSQLTKEEWKKLTKDSTTKAVQELVSSPDFGKWAAVNADRINVTPRKGSSSKNQPRKWMRWF 4D5AC8207BAB5C41 emd-nemp1a-1 4 10 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 14 unspecified role unspecified role bait bait microinjection microinjection micro-injection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2A nemp1-ran-1 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3A nemp1-ran-2 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure nemp1-ran-3 4 6 unspecified role unspecified role prey prey microinjection microinjection micro-injection P62827:p.Thr42Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:54:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr42ala' Tue Jun 30 19:16:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr42Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P62827:p.Gln69Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:06:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln69leu' Tue Jun 30 16:41:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Leu' P62827:p.Thr24Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T N Wed May 02 18:38:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr24asn' Tue Jun 30 18:36:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr24Asn' 7 unspecified role unspecified role bait bait microinjection microinjection micro-injection Q6ZQE4:p.[Ser366Glu;Ser376Glu;Ser380Glu;Ser419Glu;Ser420Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E Wed May 02 20:21:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser366glu,ser376glu,ser380glu,ser419glu,ser420glu' Tue Jun 30 17:00:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser366Glu;Ser376Glu;Ser380Glu;Ser419Glu;Ser420Glu]' c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q6ZQE4:p.[Ser366Ala;Ser376Ala;Ser380Ala;Ser419Ala;Ser420Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:22:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser366ala,ser376ala,ser380ala,ser419ala,ser420ala' Tue Jun 30 20:08:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser366Ala;Ser376Ala;Ser380Ala;Ser419Ala;Ser420Ala]' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3B and E, 5A, S3, 6D, 9B nemp1-ran-4 5 6 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P62827:p.Gln69Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 19:55:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln69leu' Tue Jun 30 15:56:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Leu' P62827:p.Thr24Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T N Wed May 02 16:46:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr24asn' Tue Jun 30 18:05:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr24Asn' xenla-embryo Xenopus laevis embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. 7 unspecified role unspecified role bait bait microinjection microinjection micro-injection n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 3C nemp1-ran-5 5 6 unspecified role unspecified role prey prey microinjection microinjection micro-injection purified purified n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P62827:p.Gln69Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 17:33:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln69leu' Tue Jun 30 16:22:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln69Leu' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 3D GTP was present in the buffer. nemp1-ran-6 4 6 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role bait bait microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4A nemp1a-ran-1 4 15 unspecified role unspecified role prey prey microinjection microinjection micro-injection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4B nemp1-ran-7 1 6 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 5B q9shq6_arath-ran2-1 4 12 unspecified role unspecified role prey prey microinjection microinjection micro-injection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait microinjection microinjection micro-injection n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 9A and B f4k567_arath-ran2-1 4 16 unspecified role unspecified role bait bait microinjection microinjection micro-injection n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey microinjection microinjection micro-injection n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 9A nemp1a-nemp1b-1 4 10 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role bait bait microinjection microinjection micro-injection c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association 2A and B nemp1-ran-8 2 13 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure lemd3-nemp1b-1 4 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey microinjection microinjection micro-injection c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2A